Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1754 and Shew_1755 are separated by 25 nucleotides Shew_1755 and Shew_1756 overlap by 1 nucleotides Shew_1756 and Shew_1757 are separated by 2 nucleotides
Shew_1754: Shew_1754 - hypothetical protein (RefSeq), at 2,006,401 to 2,006,565
_1754
Shew_1755: Shew_1755 - hydrogenase expression/synthesis, HypA (RefSeq), at 2,006,591 to 2,006,941
_1755
Shew_1756: Shew_1756 - hydrogenase expression/formation protein HypE (RefSeq), at 2,006,941 to 2,007,951
_1756
Shew_1757: Shew_1757 - hydrogenase expression/formation protein HypD (RefSeq), at 2,007,954 to 2,009,069
_1757
Position (kb)
2006
2007
2008 Strain fitness (log2 ratio)
-1
0
1 at 2005.983 kb on - strand at 2005.983 kb on - strand at 2005.994 kb on - strand at 2006.040 kb on + strand at 2006.063 kb on - strand at 2006.063 kb on - strand at 2006.064 kb on - strand at 2006.260 kb on + strand at 2006.298 kb on + strand at 2006.364 kb on + strand at 2006.364 kb on + strand at 2006.387 kb on + strand at 2006.490 kb on - strand, within Shew_1754 at 2006.556 kb on - strand at 2006.968 kb on - strand at 2007.120 kb on + strand, within Shew_1756 at 2007.195 kb on - strand, within Shew_1756 at 2007.969 kb on - strand at 2008.316 kb on - strand, within Shew_1757 at 2008.346 kb on + strand, within Shew_1757
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,005,983 - +0.1 2,005,983 - +0.3 2,005,994 - +0.4 2,006,040 + +0.1 2,006,063 - -1.1 2,006,063 - +0.5 2,006,064 - +0.3 2,006,260 + +0.2 2,006,298 + -0.6 2,006,364 + +0.1 2,006,364 + +0.2 2,006,387 + +0.3 2,006,490 - Shew_1754 0.54 -0.9 2,006,556 - -0.2 2,006,968 - +0.2 2,007,120 + Shew_1756 0.18 -0.1 2,007,195 - Shew_1756 0.25 -0.6 2,007,969 - -0.1 2,008,316 - Shew_1757 0.32 -0.3 2,008,346 + Shew_1757 0.35 +0.4
Or see this region's nucleotide sequence