Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1474 and Shew_1475 are separated by 301 nucleotides Shew_1475 and Shew_1476 are separated by 57 nucleotides Shew_1476 and Shew_1477 overlap by 8 nucleotides
Shew_1474: Shew_1474 - peptidase M23B (RefSeq), at 1,699,794 to 1,700,657
_1474
Shew_1475: Shew_1475 - hypothetical protein (RefSeq), at 1,700,959 to 1,701,648
_1475
Shew_1476: Shew_1476 - RNA polymerase sigma factor (RefSeq), at 1,701,706 to 1,702,344
_1476
Shew_1477: Shew_1477 - anti-ECFsigma factor, ChrR (RefSeq), at 1,702,337 to 1,703,086
_1477
Position (kb)
1700
1701
1702 Strain fitness (log2 ratio)
-1
0
1 at 1700.148 kb on + strand, within Shew_1474 at 1700.173 kb on - strand, within Shew_1474 at 1700.825 kb on + strand at 1701.098 kb on - strand, within Shew_1475 at 1701.248 kb on + strand, within Shew_1475 at 1701.413 kb on + strand, within Shew_1475 at 1701.744 kb on - strand at 1701.933 kb on - strand, within Shew_1476 at 1702.007 kb on - strand, within Shew_1476 at 1702.203 kb on + strand, within Shew_1476
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,700,148 + Shew_1474 0.41 -0.3 1,700,173 - Shew_1474 0.44 -0.6 1,700,825 + +0.2 1,701,098 - Shew_1475 0.20 -0.9 1,701,248 + Shew_1475 0.42 -1.0 1,701,413 + Shew_1475 0.66 -0.2 1,701,744 - +0.1 1,701,933 - Shew_1476 0.36 -0.0 1,702,007 - Shew_1476 0.47 -0.7 1,702,203 + Shew_1476 0.78 +0.2
Or see this region's nucleotide sequence