Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0099 and Shew_0100 are separated by 21 nucleotides Shew_0100 and Shew_0101 are separated by 225 nucleotides Shew_0101 and Shew_0102 are separated by 101 nucleotides Shew_0102 and Shew_0103 are separated by 206 nucleotides
Shew_0099: Shew_0099 - pseudouridine synthase Rlu family protein (RefSeq), at 110,231 to 110,905
_0099
Shew_0100: Shew_0100 - hypothetical protein (RefSeq), at 110,927 to 111,094
_0100
Shew_0101: Shew_0101 - endoribonuclease L-PSP (RefSeq), at 111,320 to 111,721
_0101
Shew_0102: Shew_0102 - hypothetical protein (RefSeq), at 111,823 to 111,978
_0102
Shew_0103: Shew_0103 - sodium/glutamate symporter (RefSeq), at 112,185 to 113,408
_0103
Position (kb) 111
112 Strain fitness (log2 ratio) -1
0
1 at 110.332 kb on + strand, within Shew_0099 at 110.361 kb on + strand, within Shew_0099 at 110.364 kb on - strand, within Shew_0099 at 110.485 kb on + strand, within Shew_0099 at 110.493 kb on - strand, within Shew_0099 at 110.569 kb on - strand, within Shew_0099 at 110.622 kb on - strand, within Shew_0099 at 110.770 kb on - strand, within Shew_0099 at 111.095 kb on - strand at 111.136 kb on + strand at 111.166 kb on - strand at 111.225 kb on + strand at 111.413 kb on - strand, within Shew_0101 at 111.429 kb on + strand, within Shew_0101 at 111.450 kb on - strand, within Shew_0101 at 111.493 kb on + strand, within Shew_0101 at 111.745 kb on + strand at 111.879 kb on + strand, within Shew_0102 at 112.067 kb on - strand at 112.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 110,332 + Shew_0099 0.15 -0.0 110,361 + Shew_0099 0.19 -0.3 110,364 - Shew_0099 0.20 +0.7 110,485 + Shew_0099 0.38 -0.8 110,493 - Shew_0099 0.39 +0.1 110,569 - Shew_0099 0.50 +0.2 110,622 - Shew_0099 0.58 +0.0 110,770 - Shew_0099 0.80 +0.5 111,095 - +0.0 111,136 + +0.1 111,166 - +0.2 111,225 + -0.2 111,413 - Shew_0101 0.23 +0.0 111,429 + Shew_0101 0.27 -0.1 111,450 - Shew_0101 0.32 -0.5 111,493 + Shew_0101 0.43 -0.6 111,745 + +1.0 111,879 + Shew_0102 0.36 +0.2 112,067 - +0.0 112,101 - -0.2
Or see this region's nucleotide sequence