Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS25590 and PFLU_RS25595 overlap by 4 nucleotides PFLU_RS25595 and PFLU_RS25600 are separated by 144 nucleotides PFLU_RS25600 and PFLU_RS25605 are separated by 140 nucleotides
PFLU_RS25590: PFLU_RS25590 - exodeoxyribonuclease V subunit beta, at 5,709,780 to 5,713,454
_RS25590
PFLU_RS25595: PFLU_RS25595 - exodeoxyribonuclease V subunit gamma, at 5,713,451 to 5,716,900
_RS25595
PFLU_RS25600: PFLU_RS25600 - 5S ribosomal RNA, at 5,717,045 to 5,717,160
_RS25600
PFLU_RS25605: PFLU_RS25605 - 23S ribosomal RNA, at 5,717,301 to 5,720,216
_RS25605
Position (kb) 5713
5714
5715
5716
5717 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5712.852 kb on + strand, within PFLU_RS25590 at 5712.853 kb on - strand, within PFLU_RS25590 at 5713.446 kb on - strand at 5714.058 kb on - strand, within PFLU_RS25595 at 5714.310 kb on - strand, within PFLU_RS25595 at 5715.194 kb on + strand, within PFLU_RS25595 at 5715.195 kb on - strand, within PFLU_RS25595 at 5715.195 kb on - strand, within PFLU_RS25595 at 5715.912 kb on + strand, within PFLU_RS25595 at 5715.912 kb on + strand, within PFLU_RS25595 at 5716.042 kb on - strand, within PFLU_RS25595 at 5716.295 kb on + strand, within PFLU_RS25595 at 5716.296 kb on - strand, within PFLU_RS25595 at 5716.296 kb on - strand, within PFLU_RS25595 at 5716.901 kb on - strand at 5716.943 kb on - strand at 5716.958 kb on + strand at 5716.958 kb on + strand at 5716.958 kb on + strand at 5716.958 kb on + strand at 5716.959 kb on - strand at 5716.959 kb on - strand at 5716.959 kb on - strand at 5716.980 kb on + strand at 5716.980 kb on + strand at 5716.980 kb on + strand at 5716.980 kb on + strand at 5716.981 kb on - strand at 5716.981 kb on - strand at 5716.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 5,712,852 + PFLU_RS25590 0.84 +0.3 5,712,853 - PFLU_RS25590 0.84 +0.3 5,713,446 - -0.5 5,714,058 - PFLU_RS25595 0.18 +0.9 5,714,310 - PFLU_RS25595 0.25 +0.3 5,715,194 + PFLU_RS25595 0.51 +0.3 5,715,195 - PFLU_RS25595 0.51 +0.9 5,715,195 - PFLU_RS25595 0.51 +0.3 5,715,912 + PFLU_RS25595 0.71 +0.3 5,715,912 + PFLU_RS25595 0.71 -0.1 5,716,042 - PFLU_RS25595 0.75 -0.6 5,716,295 + PFLU_RS25595 0.82 +1.9 5,716,296 - PFLU_RS25595 0.82 +1.9 5,716,296 - PFLU_RS25595 0.82 +0.9 5,716,901 - +0.4 5,716,943 - +0.6 5,716,958 + -2.4 5,716,958 + +0.6 5,716,958 + -2.2 5,716,958 + +0.6 5,716,959 - -3.6 5,716,959 - -0.4 5,716,959 - -3.4 5,716,980 + -2.3 5,716,980 + +3.1 5,716,980 + +3.3 5,716,980 + +0.7 5,716,981 - -1.7 5,716,981 - +0.5 5,716,981 - +1.3
Or see this region's nucleotide sequence