Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS18585

Experiment: Growth at pH8 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS18580 and PFLU_RS18585 overlap by 4 nucleotidesPFLU_RS18585 and PFLU_RS18590 are separated by 125 nucleotides PFLU_RS18580: PFLU_RS18580 - tRNA 2-thiouridine(34) synthase MnmA, at 4,205,496 to 4,206,620 _RS18580 PFLU_RS18585: PFLU_RS18585 - high frequency lysogenization protein HflD, at 4,206,617 to 4,207,240 _RS18585 PFLU_RS18590: PFLU_RS18590 - adenylosuccinate lyase, at 4,207,366 to 4,208,736 _RS18590 Position (kb) 4206 4207 4208Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4206.872 kb on + strand, within PFLU_RS18585at 4207.022 kb on + strand, within PFLU_RS18585at 4207.022 kb on + strand, within PFLU_RS18585at 4207.238 kb on + strandat 4207.238 kb on + strandat 4207.238 kb on + strandat 4207.238 kb on + strandat 4207.238 kb on + strandat 4207.239 kb on - strandat 4207.239 kb on - strandat 4207.239 kb on - strandat 4207.276 kb on + strandat 4207.277 kb on - strandat 4207.279 kb on + strandat 4207.280 kb on - strandat 4207.280 kb on - strandat 4207.280 kb on - strandat 4207.321 kb on - strandat 4207.321 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose
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4,206,872 + PFLU_RS18585 0.41 -2.4
4,207,022 + PFLU_RS18585 0.65 -0.8
4,207,022 + PFLU_RS18585 0.65 -2.5
4,207,238 + -1.6
4,207,238 + -2.3
4,207,238 + +1.6
4,207,238 + -1.4
4,207,238 + -2.4
4,207,239 - +0.6
4,207,239 - +1.5
4,207,239 - +0.7
4,207,276 + -0.4
4,207,277 - +0.2
4,207,279 + +0.2
4,207,280 - -0.8
4,207,280 - -0.6
4,207,280 - +0.3
4,207,321 - +2.8
4,207,321 - +0.2

Or see this region's nucleotide sequence