Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS17615 and PFLU_RS17620 are separated by 40 nucleotides PFLU_RS17620 and PFLU_RS17625 are separated by 2 nucleotides PFLU_RS17625 and PFLU_RS17630 are separated by 203 nucleotides PFLU_RS17630 and PFLU_RS17635 are separated by 172 nucleotides PFLU_RS17635 and PFLU_RS17640 are separated by 256 nucleotides
PFLU_RS17615: PFLU_RS17615 - DeoR/GlpR transcriptional regulator, at 4,008,736 to 4,009,521
_RS17615
PFLU_RS17620: PFLU_RS17620 - hypothetical protein, at 4,009,562 to 4,009,996
_RS17620
PFLU_RS17625: PFLU_RS17625 - hypothetical protein, at 4,009,999 to 4,010,211
_RS17625
PFLU_RS17630: PFLU_RS17630 - type 1 glutamine amidotransferase, at 4,010,415 to 4,010,978
_RS17630
PFLU_RS17635: PFLU_RS17635 - hypothetical protein, at 4,011,151 to 4,011,357
_RS17635
PFLU_RS17640: PFLU_RS17640 - hemerythrin domain-containing protein, at 4,011,614 to 4,012,090
_RS17640
Position (kb) 4010
4011 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4009.477 kb on + strand at 4009.477 kb on + strand at 4009.478 kb on - strand at 4009.487 kb on + strand at 4009.487 kb on + strand at 4009.488 kb on - strand at 4009.488 kb on - strand at 4009.531 kb on + strand at 4009.532 kb on - strand at 4009.613 kb on - strand, within PFLU_RS17620 at 4009.702 kb on - strand, within PFLU_RS17620 at 4009.759 kb on - strand, within PFLU_RS17620 at 4009.767 kb on + strand, within PFLU_RS17620 at 4009.767 kb on + strand, within PFLU_RS17620 at 4009.767 kb on + strand, within PFLU_RS17620 at 4009.768 kb on - strand, within PFLU_RS17620 at 4009.768 kb on - strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.863 kb on + strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.864 kb on - strand, within PFLU_RS17620 at 4009.985 kb on - strand at 4009.985 kb on - strand at 4010.216 kb on + strand at 4010.217 kb on - strand at 4010.286 kb on + strand at 4010.325 kb on - strand at 4010.335 kb on + strand at 4010.341 kb on - strand at 4010.515 kb on - strand, within PFLU_RS17630 at 4010.693 kb on + strand, within PFLU_RS17630 at 4010.693 kb on + strand, within PFLU_RS17630 at 4010.693 kb on + strand, within PFLU_RS17630 at 4010.693 kb on + strand, within PFLU_RS17630 at 4010.693 kb on + strand, within PFLU_RS17630 at 4010.694 kb on - strand, within PFLU_RS17630 at 4010.735 kb on + strand, within PFLU_RS17630 at 4010.814 kb on + strand, within PFLU_RS17630 at 4010.815 kb on - strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.909 kb on + strand, within PFLU_RS17630 at 4010.910 kb on - strand, within PFLU_RS17630 at 4010.910 kb on - strand, within PFLU_RS17630 at 4010.910 kb on - strand, within PFLU_RS17630 at 4010.910 kb on - strand, within PFLU_RS17630 at 4011.034 kb on - strand at 4011.034 kb on - strand at 4011.086 kb on + strand at 4011.086 kb on + strand at 4011.101 kb on + strand at 4011.101 kb on + strand at 4011.101 kb on + strand at 4011.112 kb on + strand at 4011.112 kb on + strand at 4011.112 kb on + strand at 4011.112 kb on + strand at 4011.112 kb on + strand at 4011.145 kb on + strand at 4011.145 kb on + strand at 4011.145 kb on + strand at 4011.145 kb on + strand at 4011.146 kb on - strand at 4011.146 kb on - strand at 4011.153 kb on - strand at 4011.153 kb on - strand at 4011.160 kb on - strand at 4011.160 kb on - strand at 4011.160 kb on - strand at 4011.160 kb on - strand at 4011.183 kb on + strand, within PFLU_RS17635 at 4011.256 kb on - strand, within PFLU_RS17635 at 4011.256 kb on - strand, within PFLU_RS17635 at 4011.342 kb on + strand at 4011.342 kb on + strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.343 kb on - strand at 4011.344 kb on + strand at 4011.344 kb on + strand at 4011.344 kb on + strand at 4011.344 kb on + strand at 4011.344 kb on + strand at 4011.345 kb on - strand at 4011.345 kb on - strand at 4011.345 kb on - strand at 4011.376 kb on + strand at 4011.376 kb on + strand at 4011.377 kb on - strand at 4011.377 kb on - strand at 4011.383 kb on + strand at 4011.383 kb on + strand at 4011.383 kb on + strand at 4011.384 kb on - strand at 4011.384 kb on - strand at 4011.384 kb on - strand at 4011.413 kb on - strand at 4011.539 kb on + strand at 4011.539 kb on + strand at 4011.553 kb on + strand at 4011.554 kb on - strand at 4011.554 kb on - strand at 4011.554 kb on - strand at 4011.554 kb on - strand at 4011.754 kb on + strand, within PFLU_RS17640 at 4011.754 kb on + strand, within PFLU_RS17640 at 4011.755 kb on - strand, within PFLU_RS17640 at 4011.755 kb on - strand, within PFLU_RS17640 at 4011.755 kb on - strand, within PFLU_RS17640 at 4011.755 kb on - strand, within PFLU_RS17640 at 4011.821 kb on + strand, within PFLU_RS17640 at 4011.821 kb on + strand, within PFLU_RS17640 at 4011.821 kb on + strand, within PFLU_RS17640 at 4011.821 kb on + strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.822 kb on - strand, within PFLU_RS17640 at 4011.949 kb on + strand, within PFLU_RS17640 at 4011.949 kb on + strand, within PFLU_RS17640 at 4011.949 kb on + strand, within PFLU_RS17640
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 4,009,477 + +1.5 4,009,477 + +0.9 4,009,478 - -0.5 4,009,487 + -2.5 4,009,487 + +0.7 4,009,488 - +0.0 4,009,488 - +1.7 4,009,531 + +1.2 4,009,532 - +2.3 4,009,613 - PFLU_RS17620 0.12 +0.6 4,009,702 - PFLU_RS17620 0.32 -3.4 4,009,759 - PFLU_RS17620 0.45 +1.3 4,009,767 + PFLU_RS17620 0.47 -0.8 4,009,767 + PFLU_RS17620 0.47 +1.0 4,009,767 + PFLU_RS17620 0.47 +1.3 4,009,768 - PFLU_RS17620 0.47 -2.1 4,009,768 - PFLU_RS17620 0.47 +2.0 4,009,863 + PFLU_RS17620 0.69 -1.8 4,009,863 + PFLU_RS17620 0.69 -0.5 4,009,863 + PFLU_RS17620 0.69 +1.3 4,009,863 + PFLU_RS17620 0.69 +0.2 4,009,863 + PFLU_RS17620 0.69 -2.0 4,009,863 + PFLU_RS17620 0.69 +1.0 4,009,863 + PFLU_RS17620 0.69 -2.5 4,009,864 - PFLU_RS17620 0.69 -2.5 4,009,864 - PFLU_RS17620 0.69 -3.2 4,009,864 - PFLU_RS17620 0.69 +0.2 4,009,864 - PFLU_RS17620 0.69 +0.9 4,009,864 - PFLU_RS17620 0.69 +1.0 4,009,864 - PFLU_RS17620 0.69 +1.3 4,009,864 - PFLU_RS17620 0.69 +1.0 4,009,864 - PFLU_RS17620 0.69 +0.6 4,009,985 - +0.0 4,009,985 - +0.5 4,010,216 + +0.0 4,010,217 - -0.5 4,010,286 + +0.0 4,010,325 - +0.7 4,010,335 + +0.0 4,010,341 - -2.4 4,010,515 - PFLU_RS17630 0.18 +1.7 4,010,693 + PFLU_RS17630 0.49 -1.5 4,010,693 + PFLU_RS17630 0.49 +0.1 4,010,693 + PFLU_RS17630 0.49 +2.4 4,010,693 + PFLU_RS17630 0.49 +2.5 4,010,693 + PFLU_RS17630 0.49 -1.3 4,010,694 - PFLU_RS17630 0.49 +2.2 4,010,735 + PFLU_RS17630 0.57 -0.2 4,010,814 + PFLU_RS17630 0.71 +0.0 4,010,815 - PFLU_RS17630 0.71 +1.0 4,010,909 + PFLU_RS17630 0.88 -1.8 4,010,909 + PFLU_RS17630 0.88 -2.0 4,010,909 + PFLU_RS17630 0.88 +0.9 4,010,909 + PFLU_RS17630 0.88 +2.2 4,010,909 + PFLU_RS17630 0.88 +1.8 4,010,909 + PFLU_RS17630 0.88 +1.4 4,010,910 - PFLU_RS17630 0.88 +0.9 4,010,910 - PFLU_RS17630 0.88 +1.0 4,010,910 - PFLU_RS17630 0.88 +1.0 4,010,910 - PFLU_RS17630 0.88 +0.2 4,011,034 - -1.3 4,011,034 - +0.7 4,011,086 + +0.9 4,011,086 + -1.5 4,011,101 + +1.2 4,011,101 + -1.0 4,011,101 + +0.7 4,011,112 + +0.2 4,011,112 + -0.1 4,011,112 + -0.8 4,011,112 + +2.3 4,011,112 + +1.0 4,011,145 + -1.1 4,011,145 + -0.1 4,011,145 + +0.4 4,011,145 + -0.1 4,011,146 - +0.8 4,011,146 - +1.0 4,011,153 - -1.9 4,011,153 - +0.8 4,011,160 - -1.0 4,011,160 - +0.2 4,011,160 - +0.3 4,011,160 - -2.5 4,011,183 + PFLU_RS17635 0.15 -0.3 4,011,256 - PFLU_RS17635 0.51 +0.6 4,011,256 - PFLU_RS17635 0.51 +0.0 4,011,342 + +0.6 4,011,342 + -0.2 4,011,343 - +1.0 4,011,343 - +0.0 4,011,343 - -0.5 4,011,343 - -1.3 4,011,343 - +1.0 4,011,343 - +1.3 4,011,343 - -0.7 4,011,343 - +0.2 4,011,343 - +0.6 4,011,343 - +0.8 4,011,344 + +0.0 4,011,344 + -2.9 4,011,344 + -0.3 4,011,344 + +1.5 4,011,344 + -0.4 4,011,345 - -1.3 4,011,345 - +1.5 4,011,345 - -0.9 4,011,376 + -1.8 4,011,376 + -3.1 4,011,377 - +1.3 4,011,377 - -1.1 4,011,383 + +0.0 4,011,383 + -0.4 4,011,383 + -1.7 4,011,384 - -0.3 4,011,384 - +2.3 4,011,384 - +1.5 4,011,413 - -1.0 4,011,539 + -0.6 4,011,539 + +0.0 4,011,553 + +0.7 4,011,554 - +1.6 4,011,554 - +1.0 4,011,554 - +1.7 4,011,554 - +0.2 4,011,754 + PFLU_RS17640 0.29 -0.1 4,011,754 + PFLU_RS17640 0.29 -1.5 4,011,755 - PFLU_RS17640 0.30 -0.7 4,011,755 - PFLU_RS17640 0.30 +0.9 4,011,755 - PFLU_RS17640 0.30 +0.0 4,011,755 - PFLU_RS17640 0.30 +3.0 4,011,821 + PFLU_RS17640 0.43 -1.0 4,011,821 + PFLU_RS17640 0.43 -0.2 4,011,821 + PFLU_RS17640 0.43 -2.5 4,011,821 + PFLU_RS17640 0.43 -1.3 4,011,822 - PFLU_RS17640 0.44 +0.0 4,011,822 - PFLU_RS17640 0.44 +1.0 4,011,822 - PFLU_RS17640 0.44 -0.2 4,011,822 - PFLU_RS17640 0.44 -0.2 4,011,822 - PFLU_RS17640 0.44 -3.5 4,011,822 - PFLU_RS17640 0.44 -0.4 4,011,822 - PFLU_RS17640 0.44 +1.7 4,011,822 - PFLU_RS17640 0.44 +2.6 4,011,822 - PFLU_RS17640 0.44 -1.2 4,011,949 + PFLU_RS17640 0.70 +0.4 4,011,949 + PFLU_RS17640 0.70 -1.8 4,011,949 + PFLU_RS17640 0.70 -0.7
Or see this region's nucleotide sequence