Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS05875 and PFLU_RS05880 are separated by 81 nucleotides PFLU_RS05880 and PFLU_RS05885 are separated by 84 nucleotides PFLU_RS05885 and PFLU_RS05890 are separated by 8 nucleotides PFLU_RS05890 and PFLU_RS31430 are separated by 53 nucleotides PFLU_RS31430 and PFLU_RS05895 overlap by 71 nucleotides
PFLU_RS05875: PFLU_RS05875 - lysozyme, at 1,316,948 to 1,317,448
_RS05875
PFLU_RS05880: PFLU_RS05880 - CinA family protein, at 1,317,530 to 1,318,030
_RS05880
PFLU_RS05885: PFLU_RS05885 - recombinase RecA, at 1,318,115 to 1,319,173
_RS05885
PFLU_RS05890: PFLU_RS05890 - recombination regulator RecX, at 1,319,182 to 1,319,649
_RS05890
PFLU_RS31430: PFLU_RS31430 - hypothetical protein, at 1,319,703 to 1,319,963
_RS31430
PFLU_RS05895: PFLU_RS05895 - TIGR00730 family Rossman fold protein, at 1,319,893 to 1,321,005
_RS05895
Position (kb) 1318
1319
1320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1317.180 kb on + strand, within PFLU_RS05875 at 1317.181 kb on - strand, within PFLU_RS05875 at 1317.181 kb on - strand, within PFLU_RS05875 at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.425 kb on + strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.426 kb on - strand at 1317.492 kb on + strand at 1317.492 kb on + strand at 1317.492 kb on + strand at 1317.492 kb on + strand at 1317.493 kb on - strand at 1317.497 kb on + strand at 1317.498 kb on - strand at 1317.680 kb on + strand, within PFLU_RS05880 at 1317.681 kb on - strand, within PFLU_RS05880 at 1317.689 kb on + strand, within PFLU_RS05880 at 1317.689 kb on + strand, within PFLU_RS05880 at 1317.689 kb on + strand, within PFLU_RS05880 at 1317.690 kb on - strand, within PFLU_RS05880 at 1317.690 kb on - strand, within PFLU_RS05880 at 1317.877 kb on + strand, within PFLU_RS05880 at 1317.878 kb on - strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.882 kb on + strand, within PFLU_RS05880 at 1317.883 kb on - strand, within PFLU_RS05880 at 1317.883 kb on - strand, within PFLU_RS05880 at 1317.883 kb on - strand, within PFLU_RS05880 at 1318.056 kb on + strand at 1318.057 kb on - strand at 1318.057 kb on - strand at 1318.064 kb on - strand at 1318.068 kb on - strand at 1318.068 kb on - strand at 1318.093 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.100 kb on + strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.101 kb on - strand at 1318.114 kb on - strand at 1318.189 kb on - strand at 1318.195 kb on + strand at 1318.305 kb on - strand, within PFLU_RS05885 at 1318.418 kb on + strand, within PFLU_RS05885 at 1318.419 kb on - strand, within PFLU_RS05885 at 1318.419 kb on - strand, within PFLU_RS05885 at 1318.651 kb on + strand, within PFLU_RS05885 at 1318.651 kb on + strand, within PFLU_RS05885 at 1318.652 kb on - strand, within PFLU_RS05885 at 1318.747 kb on + strand, within PFLU_RS05885 at 1318.763 kb on + strand, within PFLU_RS05885 at 1318.763 kb on + strand, within PFLU_RS05885 at 1318.763 kb on + strand, within PFLU_RS05885 at 1318.764 kb on - strand, within PFLU_RS05885 at 1318.764 kb on - strand, within PFLU_RS05885 at 1318.764 kb on - strand, within PFLU_RS05885 at 1318.764 kb on - strand, within PFLU_RS05885 at 1318.912 kb on + strand, within PFLU_RS05885 at 1318.912 kb on + strand, within PFLU_RS05885 at 1318.912 kb on + strand, within PFLU_RS05885 at 1318.912 kb on + strand, within PFLU_RS05885 at 1318.913 kb on - strand, within PFLU_RS05885 at 1318.913 kb on - strand, within PFLU_RS05885 at 1318.916 kb on + strand, within PFLU_RS05885 at 1318.916 kb on + strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.917 kb on - strand, within PFLU_RS05885 at 1318.982 kb on + strand, within PFLU_RS05885 at 1318.983 kb on - strand, within PFLU_RS05885 at 1318.988 kb on + strand, within PFLU_RS05885 at 1318.988 kb on + strand, within PFLU_RS05885 at 1318.988 kb on + strand, within PFLU_RS05885 at 1318.988 kb on + strand, within PFLU_RS05885 at 1318.989 kb on - strand, within PFLU_RS05885 at 1318.989 kb on - strand, within PFLU_RS05885 at 1318.989 kb on - strand, within PFLU_RS05885 at 1318.989 kb on - strand, within PFLU_RS05885 at 1319.192 kb on + strand at 1319.192 kb on + strand at 1319.192 kb on + strand at 1319.192 kb on + strand at 1319.192 kb on + strand at 1319.193 kb on - strand at 1319.193 kb on - strand at 1319.193 kb on - strand at 1319.193 kb on - strand at 1319.193 kb on - strand at 1319.193 kb on - strand at 1319.199 kb on + strand at 1319.199 kb on + strand at 1319.199 kb on + strand at 1319.200 kb on - strand at 1319.200 kb on - strand at 1319.200 kb on - strand at 1319.383 kb on + strand, within PFLU_RS05890 at 1319.588 kb on - strand, within PFLU_RS05890 at 1319.637 kb on + strand at 1319.637 kb on + strand at 1319.638 kb on - strand at 1319.651 kb on + strand at 1319.651 kb on + strand at 1319.652 kb on - strand at 1319.652 kb on - strand at 1319.661 kb on + strand at 1319.661 kb on + strand at 1319.661 kb on + strand at 1319.661 kb on + strand at 1319.662 kb on - strand at 1319.662 kb on - strand at 1319.662 kb on - strand at 1319.686 kb on + strand at 1319.687 kb on - strand at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.740 kb on + strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.741 kb on - strand, within PFLU_RS31430 at 1319.856 kb on - strand, within PFLU_RS31430 at 1319.856 kb on - strand, within PFLU_RS31430 at 1319.856 kb on - strand, within PFLU_RS31430 at 1319.863 kb on + strand, within PFLU_RS31430 at 1319.863 kb on + strand, within PFLU_RS31430 at 1319.864 kb on - strand, within PFLU_RS31430 at 1319.864 kb on - strand, within PFLU_RS31430 at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.966 kb on + strand at 1319.967 kb on - strand at 1319.967 kb on - strand at 1319.967 kb on - strand at 1319.967 kb on - strand at 1319.967 kb on - strand at 1319.967 kb on - strand at 1320.068 kb on + strand, within PFLU_RS05895 at 1320.068 kb on + strand, within PFLU_RS05895 at 1320.069 kb on - strand, within PFLU_RS05895
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 1,317,180 + PFLU_RS05875 0.46 +0.3 1,317,181 - PFLU_RS05875 0.47 -1.5 1,317,181 - PFLU_RS05875 0.47 +1.0 1,317,425 + +1.0 1,317,425 + -1.3 1,317,425 + +1.3 1,317,425 + -1.1 1,317,425 + +1.8 1,317,425 + +0.9 1,317,425 + +2.0 1,317,425 + -0.3 1,317,425 + +0.8 1,317,425 + +1.1 1,317,425 + -0.0 1,317,426 - +0.4 1,317,426 - -2.2 1,317,426 - -1.9 1,317,426 - -0.0 1,317,426 - +1.1 1,317,426 - -0.3 1,317,426 - -1.9 1,317,426 - -0.1 1,317,426 - -1.5 1,317,492 + +1.3 1,317,492 + +0.8 1,317,492 + -2.4 1,317,492 + -0.5 1,317,493 - +0.7 1,317,497 + +1.4 1,317,498 - -1.9 1,317,680 + PFLU_RS05880 0.30 -0.3 1,317,681 - PFLU_RS05880 0.30 -0.2 1,317,689 + PFLU_RS05880 0.32 +0.8 1,317,689 + PFLU_RS05880 0.32 +0.9 1,317,689 + PFLU_RS05880 0.32 -2.2 1,317,690 - PFLU_RS05880 0.32 -2.5 1,317,690 - PFLU_RS05880 0.32 +1.7 1,317,877 + PFLU_RS05880 0.69 -1.0 1,317,878 - PFLU_RS05880 0.69 +2.4 1,317,882 + PFLU_RS05880 0.70 -0.9 1,317,882 + PFLU_RS05880 0.70 -0.6 1,317,882 + PFLU_RS05880 0.70 -0.9 1,317,882 + PFLU_RS05880 0.70 +1.1 1,317,882 + PFLU_RS05880 0.70 -0.1 1,317,882 + PFLU_RS05880 0.70 +2.4 1,317,882 + PFLU_RS05880 0.70 +1.2 1,317,882 + PFLU_RS05880 0.70 +2.1 1,317,882 + PFLU_RS05880 0.70 +0.3 1,317,883 - PFLU_RS05880 0.70 +0.5 1,317,883 - PFLU_RS05880 0.70 -0.9 1,317,883 - PFLU_RS05880 0.70 -0.3 1,318,056 + -0.4 1,318,057 - -0.9 1,318,057 - +1.1 1,318,064 - -1.1 1,318,068 - -0.3 1,318,068 - -0.3 1,318,093 + +0.2 1,318,100 + +1.4 1,318,100 + -2.2 1,318,100 + -0.3 1,318,100 + +1.9 1,318,100 + +1.7 1,318,100 + +0.7 1,318,100 + +0.7 1,318,100 + -3.2 1,318,101 - +0.3 1,318,101 - +1.3 1,318,101 - -1.6 1,318,101 - -1.1 1,318,101 - -1.5 1,318,101 - -3.1 1,318,101 - -0.6 1,318,101 - -0.1 1,318,101 - +0.8 1,318,114 - +1.7 1,318,189 - -0.3 1,318,195 + +1.7 1,318,305 - PFLU_RS05885 0.18 +0.7 1,318,418 + PFLU_RS05885 0.29 +0.3 1,318,419 - PFLU_RS05885 0.29 -0.9 1,318,419 - PFLU_RS05885 0.29 -0.3 1,318,651 + PFLU_RS05885 0.51 +0.7 1,318,651 + PFLU_RS05885 0.51 -1.3 1,318,652 - PFLU_RS05885 0.51 -1.9 1,318,747 + PFLU_RS05885 0.60 -0.6 1,318,763 + PFLU_RS05885 0.61 -1.9 1,318,763 + PFLU_RS05885 0.61 -0.9 1,318,763 + PFLU_RS05885 0.61 -0.6 1,318,764 - PFLU_RS05885 0.61 -0.3 1,318,764 - PFLU_RS05885 0.61 -0.9 1,318,764 - PFLU_RS05885 0.61 -0.6 1,318,764 - PFLU_RS05885 0.61 -1.1 1,318,912 + PFLU_RS05885 0.75 +0.1 1,318,912 + PFLU_RS05885 0.75 +2.7 1,318,912 + PFLU_RS05885 0.75 -0.9 1,318,912 + PFLU_RS05885 0.75 -0.6 1,318,913 - PFLU_RS05885 0.75 -0.9 1,318,913 - PFLU_RS05885 0.75 -0.3 1,318,916 + PFLU_RS05885 0.76 -0.9 1,318,916 + PFLU_RS05885 0.76 -1.1 1,318,917 - PFLU_RS05885 0.76 -0.9 1,318,917 - PFLU_RS05885 0.76 +1.7 1,318,917 - PFLU_RS05885 0.76 -1.6 1,318,917 - PFLU_RS05885 0.76 -1.8 1,318,917 - PFLU_RS05885 0.76 +0.1 1,318,917 - PFLU_RS05885 0.76 -0.3 1,318,917 - PFLU_RS05885 0.76 +2.1 1,318,917 - PFLU_RS05885 0.76 +0.7 1,318,917 - PFLU_RS05885 0.76 +3.0 1,318,917 - PFLU_RS05885 0.76 -1.3 1,318,982 + PFLU_RS05885 0.82 -0.3 1,318,983 - PFLU_RS05885 0.82 -0.9 1,318,988 + PFLU_RS05885 0.82 +2.4 1,318,988 + PFLU_RS05885 0.82 -2.1 1,318,988 + PFLU_RS05885 0.82 +0.7 1,318,988 + PFLU_RS05885 0.82 -1.3 1,318,989 - PFLU_RS05885 0.83 -0.3 1,318,989 - PFLU_RS05885 0.83 -0.9 1,318,989 - PFLU_RS05885 0.83 +0.1 1,318,989 - PFLU_RS05885 0.83 +0.1 1,319,192 + -0.1 1,319,192 + +0.2 1,319,192 + +1.1 1,319,192 + -0.4 1,319,192 + -2.3 1,319,193 - +0.7 1,319,193 - +0.6 1,319,193 - -0.9 1,319,193 - +2.4 1,319,193 - -2.4 1,319,193 - -0.2 1,319,199 + +2.3 1,319,199 + +0.2 1,319,199 + +0.1 1,319,200 - +0.9 1,319,200 - -1.1 1,319,200 - -2.0 1,319,383 + PFLU_RS05890 0.43 -1.9 1,319,588 - PFLU_RS05890 0.87 +1.5 1,319,637 + +0.5 1,319,637 + +1.2 1,319,638 - -2.2 1,319,651 + +1.4 1,319,651 + -2.2 1,319,652 - -1.1 1,319,652 - -1.5 1,319,661 + -1.1 1,319,661 + -2.6 1,319,661 + -3.0 1,319,661 + +1.1 1,319,662 - -1.5 1,319,662 - +0.8 1,319,662 - -1.0 1,319,686 + +1.1 1,319,687 - -1.1 1,319,740 + PFLU_RS31430 0.14 +1.4 1,319,740 + PFLU_RS31430 0.14 +2.2 1,319,740 + PFLU_RS31430 0.14 +1.1 1,319,740 + PFLU_RS31430 0.14 +0.1 1,319,740 + PFLU_RS31430 0.14 +0.5 1,319,740 + PFLU_RS31430 0.14 -0.3 1,319,740 + PFLU_RS31430 0.14 +2.5 1,319,740 + PFLU_RS31430 0.14 -0.6 1,319,740 + PFLU_RS31430 0.14 +0.3 1,319,741 - PFLU_RS31430 0.15 +0.9 1,319,741 - PFLU_RS31430 0.15 -0.3 1,319,741 - PFLU_RS31430 0.15 +0.3 1,319,741 - PFLU_RS31430 0.15 -0.0 1,319,741 - PFLU_RS31430 0.15 +0.0 1,319,741 - PFLU_RS31430 0.15 -2.3 1,319,741 - PFLU_RS31430 0.15 -1.3 1,319,741 - PFLU_RS31430 0.15 -2.4 1,319,856 - PFLU_RS31430 0.59 +0.7 1,319,856 - PFLU_RS31430 0.59 -1.0 1,319,856 - PFLU_RS31430 0.59 +0.7 1,319,863 + PFLU_RS31430 0.61 -1.6 1,319,863 + PFLU_RS31430 0.61 +0.2 1,319,864 - PFLU_RS31430 0.62 +1.5 1,319,864 - PFLU_RS31430 0.62 +2.1 1,319,966 + +2.8 1,319,966 + +0.7 1,319,966 + -2.5 1,319,966 + -1.6 1,319,966 + -0.4 1,319,966 + -0.9 1,319,966 + -1.8 1,319,966 + -1.6 1,319,966 + -1.9 1,319,966 + +1.2 1,319,966 + +0.9 1,319,967 - -0.3 1,319,967 - -0.1 1,319,967 - -0.1 1,319,967 - -0.2 1,319,967 - -2.4 1,319,967 - +0.7 1,320,068 + PFLU_RS05895 0.16 -2.5 1,320,068 + PFLU_RS05895 0.16 +0.1 1,320,069 - PFLU_RS05895 0.16 -0.6
Or see this region's nucleotide sequence