Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12550 and LRK54_RS12555 are separated by 75 nucleotides LRK54_RS12555 and LRK54_RS12560 overlap by 31 nucleotides
LRK54_RS12550: LRK54_RS12550 - hypothetical protein, at 2,802,422 to 2,802,718
_RS12550
LRK54_RS12555: LRK54_RS12555 - transposase, at 2,802,794 to 2,803,150
_RS12555
LRK54_RS12560: LRK54_RS12560 - IS3 family transposase, at 2,803,120 to 2,803,956
_RS12560
Position (kb)
2802
2803
2804 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2801.799 kb on + strand at 2801.800 kb on - strand at 2801.800 kb on - strand at 2801.812 kb on + strand at 2801.889 kb on + strand at 2801.889 kb on + strand at 2801.889 kb on + strand at 2801.890 kb on - strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.915 kb on + strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.916 kb on - strand at 2801.925 kb on + strand at 2801.925 kb on + strand at 2801.925 kb on + strand at 2801.925 kb on + strand at 2801.925 kb on + strand at 2801.925 kb on + strand at 2801.926 kb on - strand at 2801.926 kb on - strand at 2801.926 kb on - strand at 2801.926 kb on - strand at 2801.926 kb on - strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.943 kb on + strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2801.944 kb on - strand at 2802.011 kb on - strand at 2802.069 kb on - strand at 2802.069 kb on - strand at 2802.069 kb on - strand at 2802.123 kb on + strand at 2802.124 kb on - strand at 2802.414 kb on + strand at 2802.414 kb on + strand at 2802.415 kb on - strand at 2802.415 kb on - strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.416 kb on + strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.417 kb on - strand at 2802.455 kb on - strand, within LRK54_RS12550 at 2802.518 kb on + strand, within LRK54_RS12550 at 2802.518 kb on + strand, within LRK54_RS12550 at 2802.518 kb on + strand, within LRK54_RS12550 at 2802.518 kb on + strand, within LRK54_RS12550 at 2802.518 kb on + strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.519 kb on - strand, within LRK54_RS12550 at 2802.567 kb on - strand, within LRK54_RS12550 at 2802.573 kb on + strand, within LRK54_RS12550 at 2802.573 kb on + strand, within LRK54_RS12550 at 2802.578 kb on + strand, within LRK54_RS12550 at 2802.578 kb on + strand, within LRK54_RS12550 at 2802.579 kb on - strand, within LRK54_RS12550 at 2802.579 kb on - strand, within LRK54_RS12550 at 2802.590 kb on + strand, within LRK54_RS12550 at 2802.590 kb on + strand, within LRK54_RS12550 at 2802.590 kb on + strand, within LRK54_RS12550 at 2802.590 kb on + strand, within LRK54_RS12550 at 2802.590 kb on + strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.591 kb on - strand, within LRK54_RS12550 at 2802.620 kb on + strand, within LRK54_RS12550 at 2802.645 kb on + strand, within LRK54_RS12550 at 2802.646 kb on - strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.649 kb on + strand, within LRK54_RS12550 at 2802.650 kb on - strand, within LRK54_RS12550 at 2802.650 kb on - strand, within LRK54_RS12550 at 2802.650 kb on - strand, within LRK54_RS12550 at 2802.650 kb on - strand, within LRK54_RS12550 at 2802.652 kb on + strand, within LRK54_RS12550 at 2802.653 kb on - strand, within LRK54_RS12550 at 2802.653 kb on - strand, within LRK54_RS12550 at 2802.653 kb on - strand, within LRK54_RS12550 at 2802.684 kb on + strand, within LRK54_RS12550 at 2802.684 kb on + strand, within LRK54_RS12550 at 2802.684 kb on + strand, within LRK54_RS12550 at 2802.716 kb on + strand at 2802.716 kb on + strand at 2802.716 kb on + strand at 2802.749 kb on - strand at 2802.749 kb on - strand at 2802.749 kb on - strand at 2802.749 kb on - strand at 2802.756 kb on - strand at 2802.756 kb on - strand at 2803.979 kb on + strand at 2804.083 kb on + strand at 2804.083 kb on + strand at 2804.083 kb on + strand at 2804.089 kb on + strand at 2804.089 kb on + strand at 2804.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 2,801,799 + -0.9 2,801,800 - -1.7 2,801,800 - -0.0 2,801,812 + -0.8 2,801,889 + +0.6 2,801,889 + -0.1 2,801,889 + +0.3 2,801,890 - +0.1 2,801,915 + +0.6 2,801,915 + -0.7 2,801,915 + +0.6 2,801,915 + +0.6 2,801,915 + +0.9 2,801,915 + +0.1 2,801,915 + +0.6 2,801,915 + -1.4 2,801,915 + +0.1 2,801,915 + -0.1 2,801,916 - +1.6 2,801,916 - -0.2 2,801,916 - -1.8 2,801,916 - -0.4 2,801,916 - -0.1 2,801,916 - -1.5 2,801,916 - +0.6 2,801,916 - +0.4 2,801,916 - -1.5 2,801,925 + +0.2 2,801,925 + -0.2 2,801,925 + +2.1 2,801,925 + -0.1 2,801,925 + -0.9 2,801,925 + +0.1 2,801,926 - +0.5 2,801,926 - -0.6 2,801,926 - +0.6 2,801,926 - +0.2 2,801,926 - +0.4 2,801,943 + +0.1 2,801,943 + -1.7 2,801,943 + -1.4 2,801,943 + -0.4 2,801,943 + -1.8 2,801,943 + +0.1 2,801,943 + -1.5 2,801,943 + -0.9 2,801,944 - -0.2 2,801,944 - -1.9 2,801,944 - -0.7 2,801,944 - +0.3 2,801,944 - -0.6 2,801,944 - -0.4 2,801,944 - -2.3 2,801,944 - +1.0 2,801,944 - -0.7 2,802,011 - -0.9 2,802,069 - -1.7 2,802,069 - -0.6 2,802,069 - -0.4 2,802,123 + -0.3 2,802,124 - -1.1 2,802,414 + -0.6 2,802,414 + +0.4 2,802,415 - +0.2 2,802,415 - +0.2 2,802,416 + -0.6 2,802,416 + -0.8 2,802,416 + -0.4 2,802,416 + -0.8 2,802,416 + +0.1 2,802,416 + -2.3 2,802,416 + -1.2 2,802,417 - +0.3 2,802,417 - -0.7 2,802,417 - +1.2 2,802,417 - +0.3 2,802,417 - +1.2 2,802,417 - -0.5 2,802,417 - -1.4 2,802,417 - -0.4 2,802,417 - -1.5 2,802,417 - +0.1 2,802,417 - -0.9 2,802,455 - LRK54_RS12550 0.11 -0.2 2,802,518 + LRK54_RS12550 0.32 -0.3 2,802,518 + LRK54_RS12550 0.32 -0.6 2,802,518 + LRK54_RS12550 0.32 -0.3 2,802,518 + LRK54_RS12550 0.32 -0.7 2,802,518 + LRK54_RS12550 0.32 -1.2 2,802,519 - LRK54_RS12550 0.33 +0.0 2,802,519 - LRK54_RS12550 0.33 -1.7 2,802,519 - LRK54_RS12550 0.33 +2.6 2,802,519 - LRK54_RS12550 0.33 -0.7 2,802,519 - LRK54_RS12550 0.33 -1.4 2,802,519 - LRK54_RS12550 0.33 -0.5 2,802,519 - LRK54_RS12550 0.33 -0.2 2,802,519 - LRK54_RS12550 0.33 +0.5 2,802,567 - LRK54_RS12550 0.49 +0.9 2,802,573 + LRK54_RS12550 0.51 -0.8 2,802,573 + LRK54_RS12550 0.51 +0.4 2,802,578 + LRK54_RS12550 0.53 -2.0 2,802,578 + LRK54_RS12550 0.53 +0.2 2,802,579 - LRK54_RS12550 0.53 -0.9 2,802,579 - LRK54_RS12550 0.53 +0.1 2,802,590 + LRK54_RS12550 0.57 -0.6 2,802,590 + LRK54_RS12550 0.57 -0.5 2,802,590 + LRK54_RS12550 0.57 -1.3 2,802,590 + LRK54_RS12550 0.57 -0.8 2,802,590 + LRK54_RS12550 0.57 +1.1 2,802,591 - LRK54_RS12550 0.57 +1.0 2,802,591 - LRK54_RS12550 0.57 -0.3 2,802,591 - LRK54_RS12550 0.57 -0.6 2,802,591 - LRK54_RS12550 0.57 +0.1 2,802,591 - LRK54_RS12550 0.57 +0.8 2,802,591 - LRK54_RS12550 0.57 -1.4 2,802,620 + LRK54_RS12550 0.67 -0.5 2,802,645 + LRK54_RS12550 0.75 +0.1 2,802,646 - LRK54_RS12550 0.75 -2.5 2,802,649 + LRK54_RS12550 0.76 -1.7 2,802,649 + LRK54_RS12550 0.76 -1.0 2,802,649 + LRK54_RS12550 0.76 +0.0 2,802,649 + LRK54_RS12550 0.76 -0.4 2,802,649 + LRK54_RS12550 0.76 -0.4 2,802,649 + LRK54_RS12550 0.76 +2.1 2,802,649 + LRK54_RS12550 0.76 -0.8 2,802,649 + LRK54_RS12550 0.76 +1.6 2,802,650 - LRK54_RS12550 0.77 +0.2 2,802,650 - LRK54_RS12550 0.77 -0.4 2,802,650 - LRK54_RS12550 0.77 -0.4 2,802,650 - LRK54_RS12550 0.77 -1.0 2,802,652 + LRK54_RS12550 0.77 -1.3 2,802,653 - LRK54_RS12550 0.78 +0.4 2,802,653 - LRK54_RS12550 0.78 -0.3 2,802,653 - LRK54_RS12550 0.78 -1.9 2,802,684 + LRK54_RS12550 0.88 +0.9 2,802,684 + LRK54_RS12550 0.88 -1.2 2,802,684 + LRK54_RS12550 0.88 -0.3 2,802,716 + -1.8 2,802,716 + -0.7 2,802,716 + +2.8 2,802,749 - -2.1 2,802,749 - +2.6 2,802,749 - -1.9 2,802,749 - -0.9 2,802,756 - -0.7 2,802,756 - +0.4 2,803,979 + +0.5 2,804,083 + +0.1 2,804,083 + -0.8 2,804,083 + -0.3 2,804,089 + -2.3 2,804,089 + +1.4 2,804,134 - -1.2
Or see this region's nucleotide sequence