Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04335 and LRK54_RS04340 are separated by 98 nucleotides LRK54_RS04340 and LRK54_RS04345 are separated by 5 nucleotides LRK54_RS04345 and LRK54_RS04350 overlap by 4 nucleotides LRK54_RS04350 and LRK54_RS04355 are separated by 111 nucleotides
LRK54_RS04335: LRK54_RS04335 - class I SAM-dependent methyltransferase, at 916,477 to 917,163
_RS04335
LRK54_RS04340: LRK54_RS04340 - ribosomal protein S18-alanine N-acetyltransferase, at 917,262 to 917,732
_RS04340
LRK54_RS04345: LRK54_RS04345 - hypothetical protein, at 917,738 to 918,238
_RS04345
LRK54_RS04350: LRK54_RS04350 - phosphatidylcholine/phosphatidylserine synthase, at 918,235 to 919,017
_RS04350
LRK54_RS04355: LRK54_RS04355 - DUF4124 domain-containing protein, at 919,129 to 919,551
_RS04355
Position (kb) 917
918
919 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.848 kb on - strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.264 kb on - strand at 917.323 kb on + strand, within LRK54_RS04340 at 917.323 kb on + strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.327 kb on - strand, within LRK54_RS04340 at 917.327 kb on - strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.412 kb on + strand, within LRK54_RS04340 at 917.413 kb on - strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.644 kb on + strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 917.645 kb on - strand, within LRK54_RS04340 at 918.311 kb on + strand at 918.311 kb on + strand at 918.311 kb on + strand at 918.311 kb on + strand at 918.311 kb on + strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.312 kb on - strand at 918.597 kb on - strand, within LRK54_RS04350 at 918.597 kb on - strand, within LRK54_RS04350 at 918.597 kb on - strand, within LRK54_RS04350 at 918.597 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.736 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.738 kb on - strand, within LRK54_RS04350 at 918.872 kb on - strand, within LRK54_RS04350 at 918.894 kb on - strand, within LRK54_RS04350 at 918.894 kb on - strand, within LRK54_RS04350 at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.972 kb on - strand at 918.976 kb on - strand at 918.976 kb on - strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.049 kb on + strand at 919.050 kb on - strand at 919.050 kb on - strand at 919.050 kb on - strand at 919.050 kb on - strand at 919.087 kb on + strand at 919.087 kb on + strand at 919.087 kb on + strand at 919.088 kb on - strand at 919.088 kb on - strand at 919.088 kb on - strand at 919.105 kb on + strand at 919.105 kb on + strand at 919.106 kb on - strand at 919.106 kb on - strand at 919.106 kb on - strand at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.192 kb on + strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.193 kb on - strand, within LRK54_RS04355 at 919.225 kb on + strand, within LRK54_RS04355 at 919.225 kb on + strand, within LRK54_RS04355 at 919.226 kb on - strand, within LRK54_RS04355 at 919.226 kb on - strand, within LRK54_RS04355 at 919.226 kb on - strand, within LRK54_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 916,739 + LRK54_RS04335 0.38 -0.4 916,739 + LRK54_RS04335 0.38 -0.5 916,739 + LRK54_RS04335 0.38 -0.9 916,739 + LRK54_RS04335 0.38 +0.2 916,739 + LRK54_RS04335 0.38 +0.4 916,739 + LRK54_RS04335 0.38 -0.3 916,739 + LRK54_RS04335 0.38 +0.5 916,739 + LRK54_RS04335 0.38 +0.5 916,740 - LRK54_RS04335 0.38 +0.5 916,740 - LRK54_RS04335 0.38 -0.4 916,740 - LRK54_RS04335 0.38 +0.2 916,740 - LRK54_RS04335 0.38 -2.1 916,740 - LRK54_RS04335 0.38 +0.6 916,740 - LRK54_RS04335 0.38 +0.3 916,740 - LRK54_RS04335 0.38 +0.9 916,740 - LRK54_RS04335 0.38 -0.2 916,740 - LRK54_RS04335 0.38 -0.4 916,740 - LRK54_RS04335 0.38 -0.3 916,740 - LRK54_RS04335 0.38 -1.4 916,740 - LRK54_RS04335 0.38 +0.0 916,740 - LRK54_RS04335 0.38 +1.1 916,740 - LRK54_RS04335 0.38 +0.1 916,740 - LRK54_RS04335 0.38 +0.9 916,835 + LRK54_RS04335 0.52 -0.8 916,835 + LRK54_RS04335 0.52 +0.0 916,835 + LRK54_RS04335 0.52 -1.8 916,835 + LRK54_RS04335 0.52 -0.2 916,835 + LRK54_RS04335 0.52 +0.5 916,835 + LRK54_RS04335 0.52 +0.5 916,835 + LRK54_RS04335 0.52 -0.2 916,836 - LRK54_RS04335 0.52 -1.9 916,836 - LRK54_RS04335 0.52 -1.3 916,836 - LRK54_RS04335 0.52 +0.4 916,836 - LRK54_RS04335 0.52 -0.3 916,836 - LRK54_RS04335 0.52 +0.9 916,847 + LRK54_RS04335 0.54 +0.1 916,847 + LRK54_RS04335 0.54 -0.1 916,847 + LRK54_RS04335 0.54 -1.3 916,847 + LRK54_RS04335 0.54 -0.2 916,847 + LRK54_RS04335 0.54 +0.5 916,848 - LRK54_RS04335 0.54 -0.6 916,946 + LRK54_RS04335 0.68 -0.5 916,946 + LRK54_RS04335 0.68 -1.3 916,946 + LRK54_RS04335 0.68 -0.0 916,946 + LRK54_RS04335 0.68 +0.1 916,946 + LRK54_RS04335 0.68 +0.2 916,946 + LRK54_RS04335 0.68 -0.9 916,946 + LRK54_RS04335 0.68 +0.7 916,946 + LRK54_RS04335 0.68 +0.1 916,947 - LRK54_RS04335 0.68 +0.7 916,947 - LRK54_RS04335 0.68 +1.2 916,947 - LRK54_RS04335 0.68 -0.9 916,947 - LRK54_RS04335 0.68 +0.7 916,947 - LRK54_RS04335 0.68 +1.7 916,955 + LRK54_RS04335 0.70 +0.1 916,955 + LRK54_RS04335 0.70 +0.1 916,955 + LRK54_RS04335 0.70 -0.8 916,956 - LRK54_RS04335 0.70 +0.5 916,956 - LRK54_RS04335 0.70 -0.0 916,956 - LRK54_RS04335 0.70 +1.1 916,956 - LRK54_RS04335 0.70 -0.1 917,198 + +0.1 917,198 + +0.1 917,198 + -0.9 917,198 + -0.7 917,198 + +0.6 917,198 + -1.1 917,198 + +2.4 917,198 + -0.3 917,198 + -2.9 917,198 + -0.3 917,199 - +0.2 917,199 - +0.6 917,199 - +0.1 917,199 - -0.7 917,199 - +0.3 917,199 - +1.0 917,199 - +0.1 917,264 - +0.1 917,323 + LRK54_RS04340 0.13 +0.1 917,323 + LRK54_RS04340 0.13 -1.5 917,324 - LRK54_RS04340 0.13 -1.5 917,324 - LRK54_RS04340 0.13 -0.7 917,324 - LRK54_RS04340 0.13 -0.9 917,324 - LRK54_RS04340 0.13 -3.2 917,327 - LRK54_RS04340 0.14 -0.6 917,327 - LRK54_RS04340 0.14 -2.6 917,368 + LRK54_RS04340 0.23 -1.3 917,368 + LRK54_RS04340 0.23 -3.2 917,368 + LRK54_RS04340 0.23 +0.1 917,369 - LRK54_RS04340 0.23 -1.1 917,369 - LRK54_RS04340 0.23 -0.6 917,369 - LRK54_RS04340 0.23 -1.3 917,369 - LRK54_RS04340 0.23 -2.3 917,369 - LRK54_RS04340 0.23 -0.3 917,369 - LRK54_RS04340 0.23 -1.5 917,369 - LRK54_RS04340 0.23 -1.1 917,412 + LRK54_RS04340 0.32 +0.7 917,413 - LRK54_RS04340 0.32 -2.0 917,644 + LRK54_RS04340 0.81 -1.9 917,644 + LRK54_RS04340 0.81 -2.9 917,644 + LRK54_RS04340 0.81 +1.3 917,644 + LRK54_RS04340 0.81 -1.5 917,644 + LRK54_RS04340 0.81 -0.1 917,644 + LRK54_RS04340 0.81 -2.3 917,644 + LRK54_RS04340 0.81 -3.1 917,644 + LRK54_RS04340 0.81 -1.1 917,644 + LRK54_RS04340 0.81 -2.3 917,645 - LRK54_RS04340 0.81 -1.2 917,645 - LRK54_RS04340 0.81 -1.8 917,645 - LRK54_RS04340 0.81 -2.3 917,645 - LRK54_RS04340 0.81 +1.9 917,645 - LRK54_RS04340 0.81 +0.7 917,645 - LRK54_RS04340 0.81 -1.8 917,645 - LRK54_RS04340 0.81 -0.9 917,645 - LRK54_RS04340 0.81 -0.4 918,311 + -0.3 918,311 + +0.1 918,311 + -1.1 918,311 + +1.7 918,311 + +0.1 918,312 - -2.4 918,312 - -1.9 918,312 - -0.3 918,312 - -0.7 918,312 - -2.5 918,312 - -3.1 918,312 - -1.1 918,312 - -2.7 918,312 - -3.4 918,312 - -1.3 918,312 - -2.9 918,312 - +0.1 918,312 - -0.3 918,597 - LRK54_RS04350 0.46 +0.1 918,597 - LRK54_RS04350 0.46 -0.9 918,597 - LRK54_RS04350 0.46 -0.9 918,597 - LRK54_RS04350 0.46 -0.9 918,736 - LRK54_RS04350 0.64 -1.7 918,736 - LRK54_RS04350 0.64 -0.9 918,736 - LRK54_RS04350 0.64 -0.9 918,736 - LRK54_RS04350 0.64 +0.7 918,736 - LRK54_RS04350 0.64 -1.7 918,736 - LRK54_RS04350 0.64 -1.1 918,736 - LRK54_RS04350 0.64 -1.8 918,736 - LRK54_RS04350 0.64 -2.6 918,738 - LRK54_RS04350 0.64 -2.9 918,738 - LRK54_RS04350 0.64 -1.3 918,738 - LRK54_RS04350 0.64 -1.8 918,738 - LRK54_RS04350 0.64 -1.5 918,738 - LRK54_RS04350 0.64 +0.7 918,738 - LRK54_RS04350 0.64 -0.3 918,738 - LRK54_RS04350 0.64 -1.9 918,738 - LRK54_RS04350 0.64 -0.7 918,738 - LRK54_RS04350 0.64 -1.5 918,738 - LRK54_RS04350 0.64 -1.7 918,738 - LRK54_RS04350 0.64 -1.9 918,738 - LRK54_RS04350 0.64 -2.0 918,738 - LRK54_RS04350 0.64 -1.5 918,738 - LRK54_RS04350 0.64 -0.3 918,738 - LRK54_RS04350 0.64 -2.2 918,738 - LRK54_RS04350 0.64 +0.1 918,738 - LRK54_RS04350 0.64 -0.3 918,738 - LRK54_RS04350 0.64 -2.1 918,738 - LRK54_RS04350 0.64 -0.3 918,872 - LRK54_RS04350 0.81 -2.8 918,894 - LRK54_RS04350 0.84 +0.7 918,894 - LRK54_RS04350 0.84 -1.1 918,972 - -2.7 918,972 - +0.1 918,972 - -3.3 918,972 - -2.3 918,972 - +1.7 918,972 - +0.1 918,972 - +0.1 918,972 - -1.1 918,976 - +0.6 918,976 - -1.3 919,049 + -0.9 919,049 + -1.8 919,049 + -1.3 919,049 + +0.1 919,049 + +0.1 919,049 + +0.4 919,049 + +1.7 919,049 + +0.2 919,049 + -0.6 919,050 - +0.6 919,050 - -0.0 919,050 - -0.2 919,050 - +1.6 919,087 + +0.1 919,087 + -0.6 919,087 + -3.3 919,088 - -0.3 919,088 - +1.0 919,088 - -0.3 919,105 + -1.7 919,105 + -0.3 919,106 - +0.9 919,106 - +0.1 919,106 - +0.2 919,192 + LRK54_RS04355 0.15 -1.1 919,192 + LRK54_RS04355 0.15 +0.7 919,192 + LRK54_RS04355 0.15 -1.4 919,192 + LRK54_RS04355 0.15 -0.5 919,192 + LRK54_RS04355 0.15 -1.6 919,192 + LRK54_RS04355 0.15 +0.4 919,192 + LRK54_RS04355 0.15 -0.2 919,192 + LRK54_RS04355 0.15 -0.7 919,192 + LRK54_RS04355 0.15 -1.6 919,193 - LRK54_RS04355 0.15 -0.4 919,193 - LRK54_RS04355 0.15 -0.3 919,193 - LRK54_RS04355 0.15 -0.2 919,193 - LRK54_RS04355 0.15 -1.1 919,193 - LRK54_RS04355 0.15 +0.1 919,193 - LRK54_RS04355 0.15 -2.7 919,225 + LRK54_RS04355 0.23 +0.1 919,225 + LRK54_RS04355 0.23 +2.7 919,226 - LRK54_RS04355 0.23 -1.0 919,226 - LRK54_RS04355 0.23 +0.0 919,226 - LRK54_RS04355 0.23 +1.4
Or see this region's nucleotide sequence