Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04320 and LRK54_RS04325 are separated by 15 nucleotides LRK54_RS04325 and LRK54_RS04330 are separated by 103 nucleotides LRK54_RS04330 and LRK54_RS04335 are separated by 79 nucleotides LRK54_RS04335 and LRK54_RS04340 are separated by 98 nucleotides
LRK54_RS04320: LRK54_RS04320 - uroporphyrinogen decarboxylase, at 912,026 to 913,096
_RS04320
LRK54_RS04325: LRK54_RS04325 - DUF3147 family protein, at 913,112 to 913,465
_RS04325
LRK54_RS04330: LRK54_RS04330 - valine--tRNA ligase, at 913,569 to 916,397
_RS04330
LRK54_RS04335: LRK54_RS04335 - class I SAM-dependent methyltransferase, at 916,477 to 917,163
_RS04335
LRK54_RS04340: LRK54_RS04340 - ribosomal protein S18-alanine N-acetyltransferase, at 917,262 to 917,732
_RS04340
Position (kb) 913
914
915
916
917 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.133 kb on + strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.134 kb on - strand at 913.295 kb on + strand, within LRK54_RS04325 at 913.295 kb on + strand, within LRK54_RS04325 at 913.295 kb on + strand, within LRK54_RS04325 at 913.295 kb on + strand, within LRK54_RS04325 at 913.295 kb on + strand, within LRK54_RS04325 at 913.295 kb on + strand, within LRK54_RS04325 at 913.296 kb on - strand, within LRK54_RS04325 at 913.296 kb on - strand, within LRK54_RS04325 at 913.296 kb on - strand, within LRK54_RS04325 at 913.296 kb on - strand, within LRK54_RS04325 at 913.296 kb on - strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.307 kb on + strand, within LRK54_RS04325 at 913.308 kb on - strand, within LRK54_RS04325 at 913.308 kb on - strand, within LRK54_RS04325 at 913.308 kb on - strand, within LRK54_RS04325 at 913.308 kb on - strand, within LRK54_RS04325 at 913.434 kb on + strand at 913.434 kb on + strand at 913.435 kb on - strand at 913.505 kb on + strand at 913.505 kb on + strand at 913.505 kb on + strand at 913.506 kb on - strand at 913.506 kb on - strand at 913.506 kb on - strand at 913.506 kb on - strand at 913.506 kb on - strand at 913.506 kb on - strand at 913.526 kb on + strand at 913.526 kb on + strand at 913.526 kb on + strand at 913.526 kb on + strand at 916.559 kb on + strand, within LRK54_RS04335 at 916.559 kb on + strand, within LRK54_RS04335 at 916.559 kb on + strand, within LRK54_RS04335 at 916.559 kb on + strand, within LRK54_RS04335 at 916.559 kb on + strand, within LRK54_RS04335 at 916.560 kb on - strand, within LRK54_RS04335 at 916.560 kb on - strand, within LRK54_RS04335 at 916.560 kb on - strand, within LRK54_RS04335 at 916.562 kb on + strand, within LRK54_RS04335 at 916.562 kb on + strand, within LRK54_RS04335 at 916.563 kb on - strand, within LRK54_RS04335 at 916.563 kb on - strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.735 kb on + strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.736 kb on - strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.739 kb on + strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.740 kb on - strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.835 kb on + strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.836 kb on - strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.847 kb on + strand, within LRK54_RS04335 at 916.848 kb on - strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.946 kb on + strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.947 kb on - strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.955 kb on + strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 916.956 kb on - strand, within LRK54_RS04335 at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.198 kb on + strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.199 kb on - strand at 917.264 kb on - strand at 917.323 kb on + strand, within LRK54_RS04340 at 917.323 kb on + strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.324 kb on - strand, within LRK54_RS04340 at 917.327 kb on - strand, within LRK54_RS04340 at 917.327 kb on - strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.368 kb on + strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340 at 917.369 kb on - strand, within LRK54_RS04340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 913,133 + -0.7 913,133 + -0.6 913,133 + +1.0 913,133 + -0.6 913,133 + -0.2 913,133 + +0.7 913,133 + +1.7 913,133 + -0.4 913,133 + -0.8 913,133 + +0.1 913,134 - -1.1 913,134 - +0.5 913,134 - -0.3 913,134 - -2.8 913,134 - -0.4 913,134 - +0.2 913,134 - +1.4 913,134 - +2.0 913,134 - +0.8 913,134 - -2.2 913,295 + LRK54_RS04325 0.52 +0.2 913,295 + LRK54_RS04325 0.52 +0.5 913,295 + LRK54_RS04325 0.52 +0.5 913,295 + LRK54_RS04325 0.52 +0.2 913,295 + LRK54_RS04325 0.52 +0.6 913,295 + LRK54_RS04325 0.52 +0.9 913,296 - LRK54_RS04325 0.52 +0.1 913,296 - LRK54_RS04325 0.52 +0.1 913,296 - LRK54_RS04325 0.52 +2.7 913,296 - LRK54_RS04325 0.52 +1.4 913,296 - LRK54_RS04325 0.52 +0.2 913,307 + LRK54_RS04325 0.55 -0.9 913,307 + LRK54_RS04325 0.55 -0.1 913,307 + LRK54_RS04325 0.55 -1.3 913,307 + LRK54_RS04325 0.55 -0.9 913,307 + LRK54_RS04325 0.55 -0.4 913,307 + LRK54_RS04325 0.55 -0.0 913,307 + LRK54_RS04325 0.55 -0.4 913,308 - LRK54_RS04325 0.55 +0.5 913,308 - LRK54_RS04325 0.55 -0.2 913,308 - LRK54_RS04325 0.55 -0.3 913,308 - LRK54_RS04325 0.55 -0.0 913,434 + -2.5 913,434 + +1.3 913,435 - +0.0 913,505 + +0.6 913,505 + +1.8 913,505 + -0.0 913,506 - +1.3 913,506 - -0.1 913,506 - +0.2 913,506 - -0.5 913,506 - -1.1 913,506 - +0.2 913,526 + +0.3 913,526 + -0.1 913,526 + +0.9 913,526 + -0.3 916,559 + LRK54_RS04335 0.12 +0.7 916,559 + LRK54_RS04335 0.12 +0.3 916,559 + LRK54_RS04335 0.12 +0.1 916,559 + LRK54_RS04335 0.12 +0.9 916,559 + LRK54_RS04335 0.12 +0.8 916,560 - LRK54_RS04335 0.12 -0.5 916,560 - LRK54_RS04335 0.12 -1.6 916,560 - LRK54_RS04335 0.12 -1.0 916,562 + LRK54_RS04335 0.12 +0.7 916,562 + LRK54_RS04335 0.12 +1.7 916,563 - LRK54_RS04335 0.13 -1.1 916,563 - LRK54_RS04335 0.13 -0.3 916,735 + LRK54_RS04335 0.38 -0.3 916,735 + LRK54_RS04335 0.38 +0.6 916,735 + LRK54_RS04335 0.38 -0.6 916,735 + LRK54_RS04335 0.38 +0.5 916,735 + LRK54_RS04335 0.38 +0.7 916,735 + LRK54_RS04335 0.38 -1.0 916,735 + LRK54_RS04335 0.38 -1.1 916,735 + LRK54_RS04335 0.38 +0.4 916,735 + LRK54_RS04335 0.38 +0.1 916,735 + LRK54_RS04335 0.38 -0.6 916,735 + LRK54_RS04335 0.38 +1.7 916,735 + LRK54_RS04335 0.38 +0.7 916,735 + LRK54_RS04335 0.38 -0.5 916,735 + LRK54_RS04335 0.38 +0.1 916,735 + LRK54_RS04335 0.38 -1.3 916,735 + LRK54_RS04335 0.38 +0.6 916,735 + LRK54_RS04335 0.38 -0.0 916,735 + LRK54_RS04335 0.38 +0.4 916,735 + LRK54_RS04335 0.38 +0.0 916,735 + LRK54_RS04335 0.38 -0.4 916,735 + LRK54_RS04335 0.38 +2.3 916,735 + LRK54_RS04335 0.38 +0.5 916,735 + LRK54_RS04335 0.38 +1.7 916,736 - LRK54_RS04335 0.38 -0.0 916,736 - LRK54_RS04335 0.38 +0.4 916,736 - LRK54_RS04335 0.38 -1.9 916,736 - LRK54_RS04335 0.38 +0.1 916,736 - LRK54_RS04335 0.38 +0.5 916,736 - LRK54_RS04335 0.38 +1.3 916,736 - LRK54_RS04335 0.38 -0.9 916,736 - LRK54_RS04335 0.38 -0.2 916,736 - LRK54_RS04335 0.38 +0.5 916,736 - LRK54_RS04335 0.38 +0.6 916,736 - LRK54_RS04335 0.38 +0.7 916,736 - LRK54_RS04335 0.38 +0.9 916,736 - LRK54_RS04335 0.38 +1.3 916,736 - LRK54_RS04335 0.38 +0.4 916,736 - LRK54_RS04335 0.38 +0.0 916,736 - LRK54_RS04335 0.38 -0.7 916,736 - LRK54_RS04335 0.38 -0.6 916,736 - LRK54_RS04335 0.38 -1.0 916,736 - LRK54_RS04335 0.38 -0.4 916,736 - LRK54_RS04335 0.38 +0.5 916,736 - LRK54_RS04335 0.38 -0.2 916,736 - LRK54_RS04335 0.38 +1.2 916,736 - LRK54_RS04335 0.38 +0.4 916,739 + LRK54_RS04335 0.38 -0.4 916,739 + LRK54_RS04335 0.38 -0.5 916,739 + LRK54_RS04335 0.38 -0.9 916,739 + LRK54_RS04335 0.38 +0.2 916,739 + LRK54_RS04335 0.38 +0.4 916,739 + LRK54_RS04335 0.38 -0.3 916,739 + LRK54_RS04335 0.38 +0.5 916,739 + LRK54_RS04335 0.38 +0.5 916,740 - LRK54_RS04335 0.38 +0.5 916,740 - LRK54_RS04335 0.38 -0.4 916,740 - LRK54_RS04335 0.38 +0.2 916,740 - LRK54_RS04335 0.38 -2.1 916,740 - LRK54_RS04335 0.38 +0.6 916,740 - LRK54_RS04335 0.38 +0.3 916,740 - LRK54_RS04335 0.38 +0.9 916,740 - LRK54_RS04335 0.38 -0.2 916,740 - LRK54_RS04335 0.38 -0.4 916,740 - LRK54_RS04335 0.38 -0.3 916,740 - LRK54_RS04335 0.38 -1.4 916,740 - LRK54_RS04335 0.38 +0.0 916,740 - LRK54_RS04335 0.38 +1.1 916,740 - LRK54_RS04335 0.38 +0.1 916,740 - LRK54_RS04335 0.38 +0.9 916,835 + LRK54_RS04335 0.52 -0.8 916,835 + LRK54_RS04335 0.52 +0.0 916,835 + LRK54_RS04335 0.52 -1.8 916,835 + LRK54_RS04335 0.52 -0.2 916,835 + LRK54_RS04335 0.52 +0.5 916,835 + LRK54_RS04335 0.52 +0.5 916,835 + LRK54_RS04335 0.52 -0.2 916,836 - LRK54_RS04335 0.52 -1.9 916,836 - LRK54_RS04335 0.52 -1.3 916,836 - LRK54_RS04335 0.52 +0.4 916,836 - LRK54_RS04335 0.52 -0.3 916,836 - LRK54_RS04335 0.52 +0.9 916,847 + LRK54_RS04335 0.54 +0.1 916,847 + LRK54_RS04335 0.54 -0.1 916,847 + LRK54_RS04335 0.54 -1.3 916,847 + LRK54_RS04335 0.54 -0.2 916,847 + LRK54_RS04335 0.54 +0.5 916,848 - LRK54_RS04335 0.54 -0.6 916,946 + LRK54_RS04335 0.68 -0.5 916,946 + LRK54_RS04335 0.68 -1.3 916,946 + LRK54_RS04335 0.68 -0.0 916,946 + LRK54_RS04335 0.68 +0.1 916,946 + LRK54_RS04335 0.68 +0.2 916,946 + LRK54_RS04335 0.68 -0.9 916,946 + LRK54_RS04335 0.68 +0.7 916,946 + LRK54_RS04335 0.68 +0.1 916,947 - LRK54_RS04335 0.68 +0.7 916,947 - LRK54_RS04335 0.68 +1.2 916,947 - LRK54_RS04335 0.68 -0.9 916,947 - LRK54_RS04335 0.68 +0.7 916,947 - LRK54_RS04335 0.68 +1.7 916,955 + LRK54_RS04335 0.70 +0.1 916,955 + LRK54_RS04335 0.70 +0.1 916,955 + LRK54_RS04335 0.70 -0.8 916,956 - LRK54_RS04335 0.70 +0.5 916,956 - LRK54_RS04335 0.70 -0.0 916,956 - LRK54_RS04335 0.70 +1.1 916,956 - LRK54_RS04335 0.70 -0.1 917,198 + +0.1 917,198 + +0.1 917,198 + -0.9 917,198 + -0.7 917,198 + +0.6 917,198 + -1.1 917,198 + +2.4 917,198 + -0.3 917,198 + -2.9 917,198 + -0.3 917,199 - +0.2 917,199 - +0.6 917,199 - +0.1 917,199 - -0.7 917,199 - +0.3 917,199 - +1.0 917,199 - +0.1 917,264 - +0.1 917,323 + LRK54_RS04340 0.13 +0.1 917,323 + LRK54_RS04340 0.13 -1.5 917,324 - LRK54_RS04340 0.13 -1.5 917,324 - LRK54_RS04340 0.13 -0.7 917,324 - LRK54_RS04340 0.13 -0.9 917,324 - LRK54_RS04340 0.13 -3.2 917,327 - LRK54_RS04340 0.14 -0.6 917,327 - LRK54_RS04340 0.14 -2.6 917,368 + LRK54_RS04340 0.23 -1.3 917,368 + LRK54_RS04340 0.23 -3.2 917,368 + LRK54_RS04340 0.23 +0.1 917,369 - LRK54_RS04340 0.23 -1.1 917,369 - LRK54_RS04340 0.23 -0.6 917,369 - LRK54_RS04340 0.23 -1.3 917,369 - LRK54_RS04340 0.23 -2.3 917,369 - LRK54_RS04340 0.23 -0.3 917,369 - LRK54_RS04340 0.23 -1.5 917,369 - LRK54_RS04340 0.23 -1.1
Or see this region's nucleotide sequence