Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01340 and LRK54_RS01345 are separated by 27 nucleotides LRK54_RS01345 and LRK54_RS01350 are separated by 37 nucleotides LRK54_RS01350 and LRK54_RS01355 are separated by 77 nucleotides
LRK54_RS01340: LRK54_RS01340 - amidophosphoribosyltransferase, at 290,875 to 292,350
_RS01340
LRK54_RS01345: LRK54_RS01345 - CvpA family protein, at 292,378 to 292,998
_RS01345
LRK54_RS01350: LRK54_RS01350 - SPOR domain-containing protein, at 293,036 to 294,094
_RS01350
LRK54_RS01355: LRK54_RS01355 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 294,172 to 295,446
_RS01355
Position (kb) 293
294
295 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.085 kb on + strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.086 kb on - strand, within LRK54_RS01340 at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.298 kb on + strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.299 kb on - strand at 292.324 kb on - strand at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.523 kb on + strand, within LRK54_RS01345 at 292.524 kb on - strand, within LRK54_RS01345 at 292.524 kb on - strand, within LRK54_RS01345 at 292.524 kb on - strand, within LRK54_RS01345 at 292.799 kb on + strand, within LRK54_RS01345 at 292.799 kb on + strand, within LRK54_RS01345 at 292.799 kb on + strand, within LRK54_RS01345 at 292.800 kb on - strand, within LRK54_RS01345 at 292.800 kb on - strand, within LRK54_RS01345 at 292.800 kb on - strand, within LRK54_RS01345 at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.982 kb on + strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 292.983 kb on - strand at 293.332 kb on + strand, within LRK54_RS01350 at 293.333 kb on - strand, within LRK54_RS01350 at 293.333 kb on - strand, within LRK54_RS01350 at 293.535 kb on + strand, within LRK54_RS01350 at 293.535 kb on + strand, within LRK54_RS01350 at 293.535 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.556 kb on + strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.557 kb on - strand, within LRK54_RS01350 at 293.602 kb on + strand, within LRK54_RS01350 at 293.602 kb on + strand, within LRK54_RS01350 at 293.603 kb on - strand, within LRK54_RS01350 at 293.603 kb on - strand, within LRK54_RS01350 at 293.603 kb on - strand, within LRK54_RS01350 at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.139 kb on - strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.140 kb on + strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand at 294.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 292,085 + LRK54_RS01340 0.82 -0.7 292,085 + LRK54_RS01340 0.82 -0.6 292,085 + LRK54_RS01340 0.82 -2.5 292,085 + LRK54_RS01340 0.82 -0.7 292,085 + LRK54_RS01340 0.82 -0.7 292,085 + LRK54_RS01340 0.82 -0.7 292,085 + LRK54_RS01340 0.82 +0.9 292,085 + LRK54_RS01340 0.82 -1.0 292,085 + LRK54_RS01340 0.82 -1.0 292,085 + LRK54_RS01340 0.82 -0.3 292,085 + LRK54_RS01340 0.82 +0.2 292,086 - LRK54_RS01340 0.82 -1.0 292,086 - LRK54_RS01340 0.82 -1.7 292,086 - LRK54_RS01340 0.82 -0.2 292,086 - LRK54_RS01340 0.82 -0.2 292,086 - LRK54_RS01340 0.82 -0.1 292,086 - LRK54_RS01340 0.82 -0.9 292,086 - LRK54_RS01340 0.82 +0.8 292,086 - LRK54_RS01340 0.82 +0.1 292,086 - LRK54_RS01340 0.82 -0.7 292,086 - LRK54_RS01340 0.82 -0.6 292,298 + -1.1 292,298 + +2.0 292,298 + -1.1 292,298 + +1.1 292,298 + +1.2 292,298 + -0.8 292,298 + +0.1 292,298 + +0.4 292,298 + -0.5 292,298 + -0.8 292,298 + -1.2 292,298 + +0.9 292,298 + -0.2 292,299 - +0.7 292,299 - -0.7 292,299 - -1.1 292,299 - -0.3 292,299 - +0.9 292,299 - +0.1 292,299 - -0.3 292,299 - -0.7 292,299 - -0.9 292,299 - +0.4 292,299 - +0.1 292,299 - +0.1 292,299 - -0.3 292,299 - -0.4 292,324 - -0.7 292,523 + LRK54_RS01345 0.23 -0.3 292,523 + LRK54_RS01345 0.23 -0.7 292,523 + LRK54_RS01345 0.23 -1.7 292,523 + LRK54_RS01345 0.23 -0.4 292,523 + LRK54_RS01345 0.23 -0.1 292,523 + LRK54_RS01345 0.23 +0.7 292,523 + LRK54_RS01345 0.23 -0.9 292,523 + LRK54_RS01345 0.23 -1.7 292,524 - LRK54_RS01345 0.24 +1.4 292,524 - LRK54_RS01345 0.24 +0.7 292,524 - LRK54_RS01345 0.24 -1.5 292,799 + LRK54_RS01345 0.68 +0.1 292,799 + LRK54_RS01345 0.68 -0.5 292,799 + LRK54_RS01345 0.68 -0.0 292,800 - LRK54_RS01345 0.68 -0.4 292,800 - LRK54_RS01345 0.68 -1.0 292,800 - LRK54_RS01345 0.68 +0.1 292,982 + -0.9 292,982 + -2.7 292,982 + -0.3 292,982 + +1.7 292,982 + +1.7 292,982 + -2.6 292,982 + -0.3 292,982 + -1.4 292,982 + -2.4 292,982 + -1.7 292,982 + -0.3 292,982 + -2.6 292,982 + -0.2 292,982 + -0.3 292,982 + -1.3 292,982 + -0.3 292,982 + +0.7 292,982 + +0.7 292,982 + -0.1 292,982 + -0.5 292,982 + +0.1 292,982 + -2.6 292,982 + +0.7 292,982 + +0.7 292,982 + -3.2 292,982 + -2.3 292,982 + -1.5 292,982 + -3.1 292,982 + -1.7 292,982 + -2.6 292,982 + -1.3 292,983 - -0.9 292,983 - -2.7 292,983 - -2.0 292,983 - -1.1 292,983 - -1.5 292,983 - +0.3 292,983 - -2.6 292,983 - -0.3 292,983 - -3.2 292,983 - -0.7 292,983 - +1.7 292,983 - -3.5 292,983 - -2.1 292,983 - +0.7 292,983 - -1.4 292,983 - +0.7 292,983 - -1.8 292,983 - -0.9 292,983 - -1.2 293,332 + LRK54_RS01350 0.28 -2.4 293,333 - LRK54_RS01350 0.28 -0.2 293,333 - LRK54_RS01350 0.28 -0.3 293,535 + LRK54_RS01350 0.47 -2.2 293,535 + LRK54_RS01350 0.47 -3.4 293,535 + LRK54_RS01350 0.47 -2.3 293,556 + LRK54_RS01350 0.49 -1.8 293,556 + LRK54_RS01350 0.49 -2.5 293,556 + LRK54_RS01350 0.49 -2.7 293,556 + LRK54_RS01350 0.49 -4.2 293,556 + LRK54_RS01350 0.49 -1.1 293,556 + LRK54_RS01350 0.49 -2.5 293,556 + LRK54_RS01350 0.49 -2.5 293,556 + LRK54_RS01350 0.49 -0.8 293,556 + LRK54_RS01350 0.49 -2.0 293,556 + LRK54_RS01350 0.49 -2.1 293,556 + LRK54_RS01350 0.49 -1.1 293,556 + LRK54_RS01350 0.49 -2.4 293,556 + LRK54_RS01350 0.49 -0.3 293,556 + LRK54_RS01350 0.49 -0.1 293,556 + LRK54_RS01350 0.49 -0.9 293,556 + LRK54_RS01350 0.49 -0.9 293,557 - LRK54_RS01350 0.49 -1.0 293,557 - LRK54_RS01350 0.49 -1.8 293,557 - LRK54_RS01350 0.49 +1.7 293,557 - LRK54_RS01350 0.49 -0.9 293,557 - LRK54_RS01350 0.49 -3.2 293,557 - LRK54_RS01350 0.49 -0.3 293,557 - LRK54_RS01350 0.49 -1.9 293,557 - LRK54_RS01350 0.49 +0.3 293,557 - LRK54_RS01350 0.49 +0.1 293,557 - LRK54_RS01350 0.49 -2.8 293,557 - LRK54_RS01350 0.49 -2.0 293,557 - LRK54_RS01350 0.49 -2.2 293,557 - LRK54_RS01350 0.49 -1.8 293,557 - LRK54_RS01350 0.49 -0.3 293,557 - LRK54_RS01350 0.49 -3.1 293,602 + LRK54_RS01350 0.53 -3.6 293,602 + LRK54_RS01350 0.53 -1.8 293,603 - LRK54_RS01350 0.54 -1.4 293,603 - LRK54_RS01350 0.54 -3.6 293,603 - LRK54_RS01350 0.54 -0.3 294,139 - +0.4 294,139 - +0.2 294,139 - -1.3 294,139 - -0.9 294,139 - -2.6 294,139 - -0.5 294,139 - -0.2 294,139 - -0.3 294,140 + -0.2 294,140 + -0.6 294,140 + -1.6 294,140 + +0.3 294,140 + -1.2 294,140 + -0.8 294,140 + -0.7 294,140 + -0.3 294,140 + -1.5 294,140 + -0.7 294,140 + +0.7 294,140 + -0.9 294,140 + -0.6 294,140 + -1.9 294,140 + -0.4 294,140 + +1.3 294,140 + -1.0 294,140 + -2.4 294,140 + -0.3 294,140 + -1.7 294,140 + -0.9 294,140 + -0.8 294,140 + -1.1 294,140 + +0.4 294,140 + +1.3 294,140 + -0.5 294,141 - -0.2 294,141 - -1.4 294,141 - +1.2 294,141 - -0.3 294,141 - +1.7 294,141 - -0.5 294,141 - -0.1 294,141 - +1.1 294,141 - -1.7 294,141 - -1.0 294,141 - -0.6 294,141 - +0.7 294,141 - +1.0 294,141 - -0.1 294,141 - -0.8 294,141 - +0.3 294,141 - -2.8 294,141 - +0.1 294,141 - -0.3 294,141 - -0.2
Or see this region's nucleotide sequence