Experiment: casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07475 and BPHYT_RS07480 are separated by 194 nucleotides BPHYT_RS07480 and BPHYT_RS07485 are separated by 113 nucleotides BPHYT_RS07485 and BPHYT_RS07490 are separated by 213 nucleotides
BPHYT_RS07475: BPHYT_RS07475 - nitrate reductase, at 1,698,848 to 1,700,308
_RS07475
BPHYT_RS07480: BPHYT_RS07480 - FAD-dependent oxidoreductase, at 1,700,503 to 1,701,894
_RS07480
BPHYT_RS07485: BPHYT_RS07485 - cupin, at 1,702,008 to 1,702,379
_RS07485
BPHYT_RS07490: BPHYT_RS07490 - aldehyde dehydrogenase, at 1,702,593 to 1,704,029
_RS07490
Position (kb) 1700
1701
1702 Strain fitness (log2 ratio) -1
0
1 at 1700.234 kb on + strand at 1700.360 kb on - strand at 1701.497 kb on - strand, within BPHYT_RS07480 at 1702.018 kb on + strand at 1702.019 kb on - strand at 1702.253 kb on - strand, within BPHYT_RS07485 at 1702.576 kb on + strand at 1702.578 kb on + strand at 1702.614 kb on + strand at 1702.614 kb on + strand at 1702.615 kb on - strand at 1702.831 kb on - strand, within BPHYT_RS07490
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos remove 1,700,234 + -0.6 1,700,360 - +0.1 1,701,497 - BPHYT_RS07480 0.71 -0.8 1,702,018 + -0.8 1,702,019 - +0.1 1,702,253 - BPHYT_RS07485 0.66 +0.8 1,702,576 + +0.4 1,702,578 + -0.2 1,702,614 + -1.2 1,702,614 + +1.4 1,702,615 - +0.9 1,702,831 - BPHYT_RS07490 0.17 -0.3
Or see this region's nucleotide sequence