Experiment: casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05000 and BPHYT_RS05005 are separated by 116 nucleotides BPHYT_RS05005 and BPHYT_RS05010 are separated by 161 nucleotides BPHYT_RS05010 and BPHYT_RS05015 overlap by 23 nucleotides BPHYT_RS05015 and BPHYT_RS05020 are separated by 180 nucleotides
BPHYT_RS05000: BPHYT_RS05000 - nucleotide pyrophosphatase, at 1,151,332 to 1,153,005
_RS05000
BPHYT_RS05005: BPHYT_RS05005 - hypothetical protein, at 1,153,122 to 1,153,475
_RS05005
BPHYT_RS05010: BPHYT_RS05010 - RpiR family transcriptional regulator, at 1,153,637 to 1,155,553
_RS05010
BPHYT_RS05015: BPHYT_RS05015 - 6-phosphogluconolactonase, at 1,155,531 to 1,156,235
_RS05015
BPHYT_RS05020: BPHYT_RS05020 - glucose-6-phosphate 1-dehydrogenase, at 1,156,416 to 1,157,879
_RS05020
Position (kb)
1153
1154
1155
1156 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1152.661 kb on + strand, within BPHYT_RS05000 at 1152.661 kb on + strand, within BPHYT_RS05000 at 1153.069 kb on + strand at 1153.122 kb on - strand at 1153.248 kb on - strand, within BPHYT_RS05005 at 1153.354 kb on - strand, within BPHYT_RS05005 at 1153.530 kb on - strand at 1153.530 kb on - strand at 1153.786 kb on + strand at 1154.062 kb on + strand, within BPHYT_RS05010 at 1154.089 kb on - strand, within BPHYT_RS05010 at 1154.617 kb on - strand, within BPHYT_RS05010 at 1154.617 kb on - strand, within BPHYT_RS05010 at 1154.731 kb on - strand, within BPHYT_RS05010 at 1154.731 kb on - strand, within BPHYT_RS05010 at 1155.124 kb on + strand, within BPHYT_RS05010 at 1155.367 kb on - strand at 1155.533 kb on - strand at 1156.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos remove 1,152,661 + BPHYT_RS05000 0.79 -0.3 1,152,661 + BPHYT_RS05000 0.79 +0.3 1,153,069 + +1.3 1,153,122 - +1.0 1,153,248 - BPHYT_RS05005 0.36 +0.0 1,153,354 - BPHYT_RS05005 0.66 +0.1 1,153,530 - +0.6 1,153,530 - -0.1 1,153,786 + -1.7 1,154,062 + BPHYT_RS05010 0.22 -3.6 1,154,089 - BPHYT_RS05010 0.24 -1.2 1,154,617 - BPHYT_RS05010 0.51 -3.1 1,154,617 - BPHYT_RS05010 0.51 -2.7 1,154,731 - BPHYT_RS05010 0.57 -3.3 1,154,731 - BPHYT_RS05010 0.57 -2.5 1,155,124 + BPHYT_RS05010 0.78 -2.8 1,155,367 - -1.5 1,155,533 - -2.9 1,156,290 - -1.4
Or see this region's nucleotide sequence