Experiment: Recovery broth with Bicyclomycin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18700 and BBR_RS18705 are separated by 495 nucleotides BBR_RS18705 and BBR_RS18710 are separated by 583 nucleotides
BBR_RS18700: BBR_RS18700 - PTS beta-glucoside transporter subunit EIIBCA, at 1,983,337 to 1,985,427
_RS18700
BBR_RS18705: BBR_RS18705 - alpha-D-glucose phosphate-specific phosphoglucomutase, at 1,985,923 to 1,987,599
_RS18705
BBR_RS18710: BBR_RS18710 - hypothetical protein, at 1,988,183 to 1,989,250
_RS18710
Position (kb)
1985
1986
1987
1988 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1984.986 kb on + strand, within BBR_RS18700 at 1984.993 kb on - strand, within BBR_RS18700 at 1985.037 kb on + strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.147 kb on - strand, within BBR_RS18700 at 1985.205 kb on + strand, within BBR_RS18700 at 1985.235 kb on + strand at 1985.249 kb on - strand at 1985.326 kb on - strand at 1985.382 kb on + strand at 1985.425 kb on - strand at 1985.451 kb on + strand at 1985.456 kb on + strand at 1985.456 kb on + strand at 1985.464 kb on - strand at 1985.464 kb on - strand at 1985.464 kb on - strand at 1985.469 kb on - strand at 1985.484 kb on + strand at 1985.484 kb on + strand at 1985.537 kb on + strand at 1985.550 kb on - strand at 1985.564 kb on - strand at 1985.573 kb on - strand at 1985.592 kb on - strand at 1985.618 kb on - strand at 1985.683 kb on - strand at 1985.726 kb on - strand at 1985.795 kb on + strand at 1985.808 kb on + strand at 1985.811 kb on - strand at 1985.839 kb on + strand at 1985.898 kb on + strand at 1985.921 kb on - strand at 1987.584 kb on + strand at 1987.603 kb on - strand at 1987.608 kb on - strand at 1987.608 kb on - strand at 1987.608 kb on - strand at 1987.608 kb on - strand at 1987.628 kb on + strand at 1987.633 kb on + strand at 1987.721 kb on + strand at 1987.721 kb on + strand at 1987.726 kb on + strand at 1987.731 kb on + strand at 1987.731 kb on + strand at 1987.731 kb on + strand at 1987.731 kb on + strand at 1987.734 kb on - strand at 1987.739 kb on - strand at 1987.739 kb on - strand at 1987.739 kb on - strand at 1987.739 kb on - strand at 1987.798 kb on + strand at 1987.798 kb on + strand at 1987.806 kb on - strand at 1987.842 kb on + strand at 1987.842 kb on + strand at 1987.892 kb on + strand at 1987.901 kb on - strand at 1987.901 kb on - strand at 1987.911 kb on - strand at 1987.911 kb on - strand at 1987.911 kb on - strand at 1987.978 kb on + strand at 1987.980 kb on + strand at 1987.986 kb on - strand at 1987.988 kb on - strand at 1987.996 kb on - strand at 1988.043 kb on - strand at 1988.043 kb on - strand at 1988.047 kb on + strand at 1988.047 kb on + strand at 1988.047 kb on + strand at 1988.047 kb on + strand at 1988.055 kb on - strand at 1988.055 kb on - strand at 1988.100 kb on + strand at 1988.123 kb on - strand at 1988.127 kb on + strand at 1988.160 kb on + strand at 1988.188 kb on - strand at 1988.188 kb on - strand at 1988.226 kb on - strand at 1988.226 kb on - strand at 1988.292 kb on - strand, within BBR_RS18710 at 1988.322 kb on + strand, within BBR_RS18710 at 1988.325 kb on - strand, within BBR_RS18710 at 1988.349 kb on + strand, within BBR_RS18710 at 1988.362 kb on - strand, within BBR_RS18710 at 1988.389 kb on + strand, within BBR_RS18710 at 1988.470 kb on - strand, within BBR_RS18710 at 1988.474 kb on + strand, within BBR_RS18710 at 1988.474 kb on + strand, within BBR_RS18710 at 1988.474 kb on + strand, within BBR_RS18710 at 1988.527 kb on - strand, within BBR_RS18710 at 1988.528 kb on + strand, within BBR_RS18710 at 1988.528 kb on + strand, within BBR_RS18710 at 1988.569 kb on - strand, within BBR_RS18710 at 1988.575 kb on + strand, within BBR_RS18710 at 1988.583 kb on - strand, within BBR_RS18710 at 1988.584 kb on + strand, within BBR_RS18710 at 1988.585 kb on + strand, within BBR_RS18710 at 1988.585 kb on + strand, within BBR_RS18710 at 1988.585 kb on + strand, within BBR_RS18710 at 1988.585 kb on + strand, within BBR_RS18710 at 1988.585 kb on + strand, within BBR_RS18710 at 1988.587 kb on + strand, within BBR_RS18710
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Bicyclomycin 0.05 mM remove 1,984,986 + BBR_RS18700 0.79 +5.7 1,984,993 - BBR_RS18700 0.79 +5.8 1,985,037 + BBR_RS18700 0.81 +5.9 1,985,122 - BBR_RS18700 0.85 +5.6 1,985,122 - BBR_RS18700 0.85 +5.4 1,985,122 - BBR_RS18700 0.85 +5.8 1,985,147 - BBR_RS18700 0.87 +5.5 1,985,205 + BBR_RS18700 0.89 +5.8 1,985,235 + +5.9 1,985,249 - +5.8 1,985,326 - +5.7 1,985,382 + +5.6 1,985,425 - +5.8 1,985,451 + +5.8 1,985,456 + +5.9 1,985,456 + +5.7 1,985,464 - +5.6 1,985,464 - +3.5 1,985,464 - +5.6 1,985,469 - +3.2 1,985,484 + +6.1 1,985,484 + +5.8 1,985,537 + +5.8 1,985,550 - +6.0 1,985,564 - +6.5 1,985,573 - +5.8 1,985,592 - +5.4 1,985,618 - +5.9 1,985,683 - +5.3 1,985,726 - +3.3 1,985,795 + +0.8 1,985,808 + +1.1 1,985,811 - +0.1 1,985,839 + +0.6 1,985,898 + +1.1 1,985,921 - +0.1 1,987,584 + +0.4 1,987,603 - +0.3 1,987,608 - -0.1 1,987,608 - +1.5 1,987,608 - +0.5 1,987,608 - -0.6 1,987,628 + +1.3 1,987,633 + -0.0 1,987,721 + +0.3 1,987,721 + -0.3 1,987,726 + -0.2 1,987,731 + -0.1 1,987,731 + -0.3 1,987,731 + +0.1 1,987,731 + +0.1 1,987,734 - +1.1 1,987,739 - +0.8 1,987,739 - -0.3 1,987,739 - +0.2 1,987,739 - -0.0 1,987,798 + -0.3 1,987,798 + +0.2 1,987,806 - +0.5 1,987,842 + +0.4 1,987,842 + -0.1 1,987,892 + +0.4 1,987,901 - +0.7 1,987,901 - -0.0 1,987,911 - +0.7 1,987,911 - -1.0 1,987,911 - +0.6 1,987,978 + +0.9 1,987,980 + +0.7 1,987,986 - +0.4 1,987,988 - +0.9 1,987,996 - +1.8 1,988,043 - +0.2 1,988,043 - -0.1 1,988,047 + +1.1 1,988,047 + +0.3 1,988,047 + -0.5 1,988,047 + +0.2 1,988,055 - +0.1 1,988,055 - -0.4 1,988,100 + -0.4 1,988,123 - +1.3 1,988,127 + +0.7 1,988,160 + +2.5 1,988,188 - -0.2 1,988,188 - +0.1 1,988,226 - +0.1 1,988,226 - +0.1 1,988,292 - BBR_RS18710 0.10 -0.1 1,988,322 + BBR_RS18710 0.13 +0.3 1,988,325 - BBR_RS18710 0.13 +1.9 1,988,349 + BBR_RS18710 0.16 -0.5 1,988,362 - BBR_RS18710 0.17 -0.9 1,988,389 + BBR_RS18710 0.19 +1.6 1,988,470 - BBR_RS18710 0.27 +1.4 1,988,474 + BBR_RS18710 0.27 +0.3 1,988,474 + BBR_RS18710 0.27 -0.2 1,988,474 + BBR_RS18710 0.27 +0.2 1,988,527 - BBR_RS18710 0.32 -1.5 1,988,528 + BBR_RS18710 0.32 -0.5 1,988,528 + BBR_RS18710 0.32 -0.4 1,988,569 - BBR_RS18710 0.36 +0.2 1,988,575 + BBR_RS18710 0.37 +0.7 1,988,583 - BBR_RS18710 0.37 +0.3 1,988,584 + BBR_RS18710 0.38 +1.8 1,988,585 + BBR_RS18710 0.38 +0.8 1,988,585 + BBR_RS18710 0.38 -0.1 1,988,585 + BBR_RS18710 0.38 +0.2 1,988,585 + BBR_RS18710 0.38 +0.4 1,988,585 + BBR_RS18710 0.38 -0.4 1,988,587 + BBR_RS18710 0.38 -0.6
Or see this region's nucleotide sequence