Experiment: methylglyoxal 0.016 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_09790 and AO356_09795 are separated by 63 nucleotides AO356_09795 and AO356_09800 are separated by 9 nucleotides AO356_09800 and AO356_09805 are separated by 42 nucleotides AO356_09805 and AO356_09810 overlap by 1 nucleotides AO356_09810 and AO356_09815 overlap by 1 nucleotides
AO356_09790: AO356_09790 - amino acid ABC transporter substrate-binding protein, at 2,220,001 to 2,220,747
_09790
AO356_09795: AO356_09795 - imidazole glycerol phosphate synthase subunit HisF, at 2,220,811 to 2,221,581
_09795
AO356_09800: AO356_09800 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 2,221,591 to 2,222,328
_09800
AO356_09805: AO356_09805 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 2,222,371 to 2,222,625
_09805
AO356_09810: AO356_09810 - imidazole glycerol phosphate synthase subunit HisH, at 2,222,625 to 2,223,263
_09810
AO356_09815: AO356_09815 - imidazoleglycerol-phosphate dehydratase, at 2,223,263 to 2,223,856
_09815
Position (kb)
2221
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2220.638 kb on + strand, within AO356_09790 at 2220.639 kb on - strand, within AO356_09790 at 2220.675 kb on + strand at 2220.675 kb on + strand at 2220.675 kb on + strand at 2220.676 kb on - strand at 2220.676 kb on - strand at 2220.842 kb on + strand at 2220.842 kb on + strand at 2220.842 kb on + strand at 2220.842 kb on + strand at 2220.842 kb on + strand at 2220.867 kb on - strand at 2221.386 kb on + strand, within AO356_09795 at 2221.387 kb on - strand, within AO356_09795 at 2221.602 kb on - strand at 2221.649 kb on + strand at 2221.649 kb on + strand at 2221.833 kb on - strand, within AO356_09800 at 2221.888 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.285 kb on - strand at 2222.345 kb on + strand at 2222.345 kb on + strand at 2222.346 kb on - strand at 2222.346 kb on - strand at 2222.346 kb on - strand at 2222.351 kb on + strand at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.626 kb on + strand at 2222.626 kb on + strand at 2222.627 kb on - strand at 2222.678 kb on + strand at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.816 kb on - strand, within AO356_09810 at 2222.816 kb on - strand, within AO356_09810 at 2223.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.016 vol% remove 2,220,638 + AO356_09790 0.85 +1.0 2,220,639 - AO356_09790 0.85 +1.2 2,220,675 + -0.4 2,220,675 + +0.9 2,220,675 + +0.9 2,220,676 - +0.3 2,220,676 - +1.2 2,220,842 + +2.3 2,220,842 + +0.2 2,220,842 + +1.9 2,220,842 + +1.6 2,220,842 + +2.7 2,220,867 - +0.8 2,221,386 + AO356_09795 0.75 +1.2 2,221,387 - AO356_09795 0.75 +1.2 2,221,602 - +0.5 2,221,649 + +1.2 2,221,649 + +3.7 2,221,833 - AO356_09800 0.33 -0.0 2,221,888 + AO356_09800 0.40 +1.2 2,222,030 + AO356_09800 0.59 -2.2 2,222,030 + AO356_09800 0.59 +1.2 2,222,030 + AO356_09800 0.59 +2.8 2,222,030 + AO356_09800 0.59 +1.1 2,222,030 + AO356_09800 0.59 +1.3 2,222,051 + AO356_09800 0.62 +0.9 2,222,051 + AO356_09800 0.62 +2.4 2,222,051 + AO356_09800 0.62 +1.4 2,222,051 + AO356_09800 0.62 +1.2 2,222,051 + AO356_09800 0.62 +2.8 2,222,052 - AO356_09800 0.62 +1.8 2,222,052 - AO356_09800 0.62 -0.1 2,222,052 - AO356_09800 0.62 +2.8 2,222,052 - AO356_09800 0.62 +1.5 2,222,284 + +1.4 2,222,284 + +1.3 2,222,284 + +1.3 2,222,284 + +0.6 2,222,285 - +0.1 2,222,345 + +2.9 2,222,345 + +1.4 2,222,346 - -0.0 2,222,346 - -1.1 2,222,346 - -1.2 2,222,351 + +1.9 2,222,460 - AO356_09805 0.35 +0.6 2,222,460 - AO356_09805 0.35 +0.7 2,222,460 - AO356_09805 0.35 -1.1 2,222,460 - AO356_09805 0.35 -0.4 2,222,460 - AO356_09805 0.35 +0.7 2,222,626 + +1.3 2,222,626 + +0.1 2,222,627 - -0.2 2,222,678 + +2.3 2,222,797 + AO356_09810 0.27 -0.1 2,222,797 + AO356_09810 0.27 +1.1 2,222,797 + AO356_09810 0.27 +0.4 2,222,797 + AO356_09810 0.27 -1.7 2,222,798 - AO356_09810 0.27 +2.5 2,222,798 - AO356_09810 0.27 -1.1 2,222,798 - AO356_09810 0.27 +2.5 2,222,798 - AO356_09810 0.27 +1.5 2,222,798 - AO356_09810 0.27 +2.5 2,222,800 + AO356_09810 0.27 -0.1 2,222,800 + AO356_09810 0.27 +1.4 2,222,800 + AO356_09810 0.27 +0.9 2,222,800 + AO356_09810 0.27 +2.6 2,222,800 + AO356_09810 0.27 +2.0 2,222,800 + AO356_09810 0.27 +2.6 2,222,800 + AO356_09810 0.27 +1.3 2,222,801 - AO356_09810 0.28 +2.6 2,222,801 - AO356_09810 0.28 +1.8 2,222,801 - AO356_09810 0.28 +1.0 2,222,801 - AO356_09810 0.28 -1.6 2,222,816 - AO356_09810 0.30 +1.3 2,222,816 - AO356_09810 0.30 +2.4 2,223,264 + +2.5
Or see this region's nucleotide sequence