Experiment: methylglyoxal 0.016 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02305 and AO356_02310 are separated by 106 nucleotides AO356_02310 and AO356_02315 are separated by 236 nucleotides AO356_02315 and AO356_02320 are separated by 115 nucleotides AO356_02320 and AO356_02325 are separated by 108 nucleotides
AO356_02305: AO356_02305 - hypothetical protein, at 498,139 to 498,372
_02305
AO356_02310: AO356_02310 - PasA protein, at 498,479 to 499,003
_02310
AO356_02315: AO356_02315 - DNA topoisomerase I, at 499,240 to 501,852
_02315
AO356_02320: AO356_02320 - TonB box-like protein, at 501,968 to 502,204
_02320
AO356_02325: AO356_02325 - 3-ketoacyl-CoA thiolase, at 502,313 to 503,488
_02325
Position (kb) 499
500
501
502 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 498.259 kb on + strand, within AO356_02305 at 498.259 kb on + strand, within AO356_02305 at 498.259 kb on + strand, within AO356_02305 at 498.260 kb on - strand, within AO356_02305 at 498.366 kb on + strand at 498.366 kb on + strand at 498.366 kb on + strand at 498.366 kb on + strand at 498.372 kb on + strand at 498.372 kb on + strand at 498.372 kb on + strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.460 kb on + strand at 498.461 kb on - strand at 498.680 kb on + strand, within AO356_02310 at 498.680 kb on + strand, within AO356_02310 at 498.681 kb on - strand, within AO356_02310 at 498.855 kb on + strand, within AO356_02310 at 498.856 kb on - strand, within AO356_02310 at 498.963 kb on + strand at 498.987 kb on + strand at 498.987 kb on + strand at 498.987 kb on + strand at 498.988 kb on - strand at 499.107 kb on + strand at 499.107 kb on + strand at 499.107 kb on + strand at 499.108 kb on - strand at 499.149 kb on + strand at 499.150 kb on - strand at 499.158 kb on + strand at 499.158 kb on + strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.281 kb on - strand at 499.281 kb on - strand at 499.281 kb on - strand at 499.325 kb on + strand at 499.410 kb on - strand at 499.532 kb on + strand, within AO356_02315 at 499.532 kb on + strand, within AO356_02315 at 499.532 kb on + strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 501.915 kb on - strand at 501.915 kb on - strand at 501.915 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.921 kb on - strand at 501.921 kb on - strand at 502.092 kb on + strand, within AO356_02320 at 502.093 kb on - strand, within AO356_02320 at 502.104 kb on + strand, within AO356_02320 at 502.104 kb on + strand, within AO356_02320 at 502.105 kb on - strand, within AO356_02320 at 502.151 kb on + strand, within AO356_02320 at 502.151 kb on + strand, within AO356_02320 at 502.152 kb on - strand, within AO356_02320 at 502.152 kb on - strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.196 kb on + strand at 502.252 kb on + strand at 502.253 kb on - strand at 502.255 kb on + strand at 502.314 kb on + strand at 502.424 kb on - strand at 502.481 kb on - strand, within AO356_02325 at 502.633 kb on - strand, within AO356_02325
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.016 vol% remove 498,259 + AO356_02305 0.51 +1.2 498,259 + AO356_02305 0.51 -1.3 498,259 + AO356_02305 0.51 +0.4 498,260 - AO356_02305 0.52 +1.1 498,366 + -0.1 498,366 + +1.2 498,366 + +0.2 498,366 + -0.4 498,372 + +0.2 498,372 + +0.6 498,372 + +0.5 498,373 - +0.7 498,373 - +1.4 498,373 - +1.4 498,373 - +2.1 498,373 - +1.4 498,373 - +1.3 498,373 - -0.0 498,403 + +1.6 498,403 + +0.6 498,403 + -0.3 498,403 + +1.7 498,404 - -1.7 498,404 - -0.6 498,404 - +0.8 498,404 - +0.4 498,405 + +1.0 498,405 + -0.5 498,405 + -0.3 498,405 + +1.0 498,405 + +0.6 498,405 + -1.3 498,405 + +0.6 498,405 + +1.2 498,405 + +1.5 498,406 - -1.2 498,406 - +0.4 498,406 - +1.0 498,406 - -0.9 498,406 - +0.0 498,406 - +0.9 498,406 - -1.7 498,406 - +0.8 498,406 - +0.2 498,406 - -1.5 498,406 - +0.9 498,406 - +1.0 498,406 - -2.6 498,407 + +1.0 498,407 + -0.2 498,407 + -2.8 498,407 + -0.7 498,408 - +0.2 498,408 - +0.7 498,408 - -0.5 498,408 - +0.8 498,408 - +0.3 498,460 + +1.7 498,461 - -1.4 498,680 + AO356_02310 0.38 +0.1 498,680 + AO356_02310 0.38 -1.0 498,681 - AO356_02310 0.38 -0.2 498,855 + AO356_02310 0.72 +0.5 498,856 - AO356_02310 0.72 +0.5 498,963 + +1.8 498,987 + +0.5 498,987 + +0.2 498,987 + -0.5 498,988 - +0.7 499,107 + +1.0 499,107 + +1.4 499,107 + -0.3 499,108 - -1.5 499,149 + -0.4 499,150 - +0.1 499,158 + -1.0 499,158 + +0.5 499,159 - -1.2 499,159 - +0.3 499,159 - +1.4 499,159 - +1.2 499,281 - -1.5 499,281 - -0.1 499,281 - -1.3 499,325 + +0.8 499,410 - -2.2 499,532 + AO356_02315 0.11 -1.1 499,532 + AO356_02315 0.11 -2.7 499,532 + AO356_02315 0.11 -1.5 499,533 - AO356_02315 0.11 -2.5 499,533 - AO356_02315 0.11 +0.5 499,533 - AO356_02315 0.11 -1.3 499,533 - AO356_02315 0.11 -2.0 499,533 - AO356_02315 0.11 -0.8 501,915 - +1.5 501,915 - +1.8 501,915 - -0.6 501,917 - +1.9 501,917 - +0.3 501,917 - +1.8 501,917 - +0.6 501,917 - +1.2 501,917 - +0.4 501,917 - -0.7 501,917 - -0.8 501,917 - +1.4 501,917 - -1.1 501,919 - -0.0 501,919 - +0.9 501,919 - +0.8 501,919 - +0.6 501,919 - +1.0 501,919 - -0.0 501,919 - +1.5 501,919 - +1.1 501,919 - -1.7 501,919 - +0.6 501,919 - +0.5 501,919 - +0.8 501,919 - +0.4 501,919 - +1.5 501,919 - +1.1 501,919 - +0.5 501,919 - +1.5 501,919 - -0.1 501,921 - +1.7 501,921 - +1.7 502,092 + AO356_02320 0.52 -1.4 502,093 - AO356_02320 0.53 +1.8 502,104 + AO356_02320 0.57 -0.2 502,104 + AO356_02320 0.57 -0.2 502,105 - AO356_02320 0.58 +1.8 502,151 + AO356_02320 0.77 -1.1 502,151 + AO356_02320 0.77 -2.0 502,152 - AO356_02320 0.78 +1.0 502,152 - AO356_02320 0.78 +2.0 502,173 + AO356_02320 0.86 -1.2 502,173 + AO356_02320 0.86 -0.5 502,173 + AO356_02320 0.86 -0.8 502,173 + AO356_02320 0.86 -0.4 502,173 + AO356_02320 0.86 -0.9 502,173 + AO356_02320 0.86 +0.4 502,173 + AO356_02320 0.86 -0.6 502,174 - AO356_02320 0.87 +1.1 502,174 - AO356_02320 0.87 +1.4 502,174 - AO356_02320 0.87 +1.4 502,196 + -0.4 502,252 + -1.9 502,253 - +1.3 502,255 + -0.5 502,314 + -0.8 502,424 - +1.0 502,481 - AO356_02325 0.14 +1.7 502,633 - AO356_02325 0.27 +1.1
Or see this region's nucleotide sequence