Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2973 and DvMF_2974 are separated by 87 nucleotides DvMF_2974 and DvMF_2975 are separated by 288 nucleotides DvMF_2975 and DvMF_2976 are separated by 1 nucleotides DvMF_2976 and DvMF_2977 are separated by 113 nucleotides
DvMF_2973: DvMF_2973 - protein of unknown function DUF6 transmembrane (RefSeq), at 3,749,108 to 3,750,070
_2973
DvMF_2974: DvMF_2974 - transcriptional regulator, HxlR family (RefSeq), at 3,750,158 to 3,750,640
_2974
DvMF_2975: DvMF_2975 - nitroreductase (RefSeq), at 3,750,929 to 3,751,438
_2975
DvMF_2976: DvMF_2976 - 4-oxalocrotonate tautomerase (RefSeq), at 3,751,440 to 3,751,628
_2976
DvMF_2977: DvMF_2977 - NADH:flavin oxidoreductase/NADH oxidase (RefSeq), at 3,751,742 to 3,752,860
_2977
Position (kb)
3750
3751
3752 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3749.980 kb on - strand at 3749.989 kb on - strand at 3749.999 kb on - strand at 3750.179 kb on + strand at 3750.477 kb on - strand, within DvMF_2974 at 3750.521 kb on - strand, within DvMF_2974 at 3750.622 kb on - strand at 3750.993 kb on - strand, within DvMF_2975 at 3751.030 kb on + strand, within DvMF_2975 at 3751.058 kb on - strand, within DvMF_2975 at 3751.223 kb on + strand, within DvMF_2975 at 3751.264 kb on - strand, within DvMF_2975 at 3751.285 kb on - strand, within DvMF_2975 at 3752.360 kb on - strand, within DvMF_2977 at 3752.384 kb on - strand, within DvMF_2977
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 3,749,980 - -0.0 3,749,989 - -0.5 3,749,999 - -0.7 3,750,179 + -2.7 3,750,477 - DvMF_2974 0.66 -0.1 3,750,521 - DvMF_2974 0.75 -1.3 3,750,622 - -3.3 3,750,993 - DvMF_2975 0.13 +2.1 3,751,030 + DvMF_2975 0.20 -0.9 3,751,058 - DvMF_2975 0.25 +0.2 3,751,223 + DvMF_2975 0.58 -0.9 3,751,264 - DvMF_2975 0.66 +1.5 3,751,285 - DvMF_2975 0.70 +0.0 3,752,360 - DvMF_2977 0.55 -1.0 3,752,384 - DvMF_2977 0.57 -0.5
Or see this region's nucleotide sequence