Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2009 and DvMF_2010 are separated by 243 nucleotides DvMF_2010 and DvMF_2011 are separated by 30 nucleotides DvMF_2011 and DvMF_2012 overlap by 47 nucleotides DvMF_2012 and DvMF_2013 are separated by 104 nucleotides
DvMF_2009: DvMF_2009 - flagellar basal body-associated protein FliL (RefSeq), at 2,488,545 to 2,489,330
_2009
DvMF_2010: DvMF_2010 - response regulator receiver protein (RefSeq), at 2,489,574 to 2,489,954
_2010
DvMF_2011: DvMF_2011 - RNA polymerase, sigma 28 subunit, FliA/WhiG (RefSeq), at 2,489,985 to 2,490,782
_2011
DvMF_2012: DvMF_2012 - Cobyrinic acid ac-diamide synthase (RefSeq), at 2,490,736 to 2,491,551
_2012
DvMF_2013: DvMF_2013 - flagellar biosynthesis protein FlhF, putative (RefSeq), at 2,491,656 to 2,492,915
_2013
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-1
0
1 at 2489.098 kb on - strand, within DvMF_2009 at 2489.105 kb on - strand, within DvMF_2009 at 2489.317 kb on + strand at 2489.391 kb on - strand at 2489.624 kb on + strand, within DvMF_2010 at 2489.970 kb on - strand at 2490.560 kb on + strand, within DvMF_2011 at 2490.657 kb on + strand, within DvMF_2011 at 2491.654 kb on + strand at 2491.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 2,489,098 - DvMF_2009 0.70 -0.8 2,489,105 - DvMF_2009 0.71 -0.3 2,489,317 + +0.4 2,489,391 - -0.4 2,489,624 + DvMF_2010 0.13 -1.6 2,489,970 - -0.9 2,490,560 + DvMF_2011 0.72 +0.7 2,490,657 + DvMF_2011 0.84 +1.0 2,491,654 + -0.4 2,491,662 - -0.8
Or see this region's nucleotide sequence