Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1171 and DvMF_1172 are separated by 124 nucleotides DvMF_1172 and DvMF_1173 are separated by 148 nucleotides DvMF_1173 and DvMF_1174 are separated by 340 nucleotides
DvMF_1171: DvMF_1171 - C_GCAxxG_C_C family protein (RefSeq), at 1,448,325 to 1,449,098
_1171
DvMF_1172: DvMF_1172 - Rhodanese domain protein (RefSeq), at 1,449,223 to 1,449,744
_1172
DvMF_1173: DvMF_1173 - hypothetical protein (RefSeq), at 1,449,893 to 1,450,369
_1173
DvMF_1174: DvMF_1174 - cobalamin (vitamin B12) biosynthesis CbiD protein (RefSeq), at 1,450,710 to 1,452,059
_1174
Position (kb)
1449
1450
1451 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1448.919 kb on - strand, within DvMF_1171 at 1448.971 kb on - strand, within DvMF_1171 at 1449.000 kb on + strand, within DvMF_1171 at 1449.244 kb on + strand at 1449.244 kb on + strand at 1449.252 kb on - strand at 1449.252 kb on - strand at 1449.299 kb on - strand, within DvMF_1172 at 1449.346 kb on - strand, within DvMF_1172 at 1449.649 kb on - strand, within DvMF_1172 at 1449.687 kb on + strand, within DvMF_1172 at 1449.695 kb on - strand at 1449.952 kb on + strand, within DvMF_1173 at 1449.960 kb on - strand, within DvMF_1173 at 1450.004 kb on + strand, within DvMF_1173 at 1450.200 kb on - strand, within DvMF_1173 at 1450.872 kb on - strand, within DvMF_1174 at 1451.043 kb on - strand, within DvMF_1174 at 1451.337 kb on - strand, within DvMF_1174
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 1,448,919 - DvMF_1171 0.77 -3.0 1,448,971 - DvMF_1171 0.83 -0.0 1,449,000 + DvMF_1171 0.87 +0.8 1,449,244 + -1.1 1,449,244 + -2.2 1,449,252 - -2.2 1,449,252 - -2.3 1,449,299 - DvMF_1172 0.15 -0.9 1,449,346 - DvMF_1172 0.24 -1.3 1,449,649 - DvMF_1172 0.82 -1.6 1,449,687 + DvMF_1172 0.89 +0.2 1,449,695 - -1.0 1,449,952 + DvMF_1173 0.12 -1.2 1,449,960 - DvMF_1173 0.14 -0.9 1,450,004 + DvMF_1173 0.23 -2.1 1,450,200 - DvMF_1173 0.64 +0.9 1,450,872 - DvMF_1174 0.12 -1.0 1,451,043 - DvMF_1174 0.25 -0.4 1,451,337 - DvMF_1174 0.46 -3.3
Or see this region's nucleotide sequence