Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0674 and DvMF_0675 are separated by 69 nucleotides DvMF_0675 and DvMF_0676 are separated by 10 nucleotides DvMF_0676 and DvMF_0677 overlap by 4 nucleotides
DvMF_0674: DvMF_0674 - hypothetical protein (RefSeq), at 813,869 to 814,453
_0674
DvMF_0675: DvMF_0675 - Acetyltransferase (isoleucine patch superfamily)-like protein (RefSeq), at 814,523 to 815,326
_0675
DvMF_0676: DvMF_0676 - glycosyl transferase group 1 (RefSeq), at 815,337 to 816,461
_0676
DvMF_0677: DvMF_0677 - glycosyl transferase group 1 (RefSeq), at 816,458 to 817,651
_0677
Position (kb) 815
816
817 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 814.337 kb on - strand, within DvMF_0674 at 814.393 kb on + strand, within DvMF_0674 at 814.544 kb on + strand at 814.679 kb on - strand, within DvMF_0675 at 815.541 kb on + strand, within DvMF_0676 at 815.568 kb on - strand, within DvMF_0676 at 815.575 kb on - strand, within DvMF_0676 at 815.662 kb on + strand, within DvMF_0676 at 816.000 kb on - strand, within DvMF_0676 at 816.273 kb on + strand, within DvMF_0676 at 816.281 kb on - strand, within DvMF_0676 at 816.419 kb on + strand at 816.456 kb on + strand at 816.727 kb on + strand, within DvMF_0677 at 816.727 kb on + strand, within DvMF_0677 at 817.009 kb on - strand, within DvMF_0677 at 817.207 kb on - strand, within DvMF_0677 at 817.259 kb on + strand, within DvMF_0677 at 817.266 kb on + strand, within DvMF_0677 at 817.374 kb on - strand, within DvMF_0677
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 814,337 - DvMF_0674 0.80 +0.1 814,393 + DvMF_0674 0.90 -0.7 814,544 + -2.0 814,679 - DvMF_0675 0.19 -2.5 815,541 + DvMF_0676 0.18 -0.0 815,568 - DvMF_0676 0.21 -1.9 815,575 - DvMF_0676 0.21 -0.6 815,662 + DvMF_0676 0.29 -2.2 816,000 - DvMF_0676 0.59 -4.2 816,273 + DvMF_0676 0.83 -1.5 816,281 - DvMF_0676 0.84 +0.1 816,419 + +0.2 816,456 + -1.3 816,727 + DvMF_0677 0.23 +0.4 816,727 + DvMF_0677 0.23 -0.3 817,009 - DvMF_0677 0.46 -0.5 817,207 - DvMF_0677 0.63 -1.3 817,259 + DvMF_0677 0.67 -2.4 817,266 + DvMF_0677 0.68 -3.6 817,374 - DvMF_0677 0.77 -2.1
Or see this region's nucleotide sequence