Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0442 and DvMF_0443 are separated by 96 nucleotides DvMF_0443 and DvMF_0444 are separated by 9 nucleotides DvMF_0444 and DvMF_0445 are separated by 180 nucleotides
DvMF_0442: DvMF_0442 - Phenylacetate--CoA ligase (RefSeq), at 518,327 to 519,631
_0442
DvMF_0443: DvMF_0443 - hypothetical protein (RefSeq), at 519,728 to 520,174
_0443
DvMF_0444: DvMF_0444 - hypothetical protein (RefSeq), at 520,184 to 520,546
_0444
DvMF_0445: DvMF_0445 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 520,727 to 521,233
_0445
Position (kb) 519
520
521 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 518.769 kb on - strand, within DvMF_0442 at 518.857 kb on + strand, within DvMF_0442 at 518.870 kb on - strand, within DvMF_0442 at 518.878 kb on - strand, within DvMF_0442 at 518.888 kb on - strand, within DvMF_0442 at 519.024 kb on + strand, within DvMF_0442 at 519.154 kb on - strand, within DvMF_0442 at 519.180 kb on + strand, within DvMF_0442 at 519.203 kb on - strand, within DvMF_0442 at 519.887 kb on - strand, within DvMF_0443 at 519.897 kb on - strand, within DvMF_0443 at 520.197 kb on - strand at 520.906 kb on + strand, within DvMF_0445 at 521.055 kb on - strand, within DvMF_0445 at 521.122 kb on - strand, within DvMF_0445
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 518,769 - DvMF_0442 0.34 -0.0 518,857 + DvMF_0442 0.41 -0.7 518,870 - DvMF_0442 0.42 -0.0 518,878 - DvMF_0442 0.42 -0.5 518,888 - DvMF_0442 0.43 -0.0 519,024 + DvMF_0442 0.53 -3.9 519,154 - DvMF_0442 0.63 -4.0 519,180 + DvMF_0442 0.65 -1.4 519,203 - DvMF_0442 0.67 -1.3 519,887 - DvMF_0443 0.36 -0.1 519,897 - DvMF_0443 0.38 +0.3 520,197 - -1.1 520,906 + DvMF_0445 0.35 -1.1 521,055 - DvMF_0445 0.65 +0.4 521,122 - DvMF_0445 0.78 +0.4
Or see this region's nucleotide sequence