Experiment: MoYLS4 with methylglyoxal 0.031 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0017 and DvMF_0018 are separated by 3 nucleotides DvMF_0018 and DvMF_0019 are separated by 37 nucleotides DvMF_0019 and DvMF_0020 are separated by 136 nucleotides
DvMF_0017: DvMF_0017 - Methyltransferase type 11 (RefSeq), at 22,694 to 23,458
_0017
DvMF_0018: DvMF_0018 - adenylylsulfate kinase (RefSeq), at 23,462 to 24,049
_0018
DvMF_0019: DvMF_0019 - hypothetical protein (RefSeq), at 24,087 to 25,421
_0019
DvMF_0020: DvMF_0020 - hypothetical protein (RefSeq), at 25,558 to 26,634
_0020
Position (kb) 24
25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 23.304 kb on + strand, within DvMF_0017 at 23.334 kb on + strand, within DvMF_0017 at 23.460 kb on - strand at 23.723 kb on - strand, within DvMF_0018 at 24.058 kb on + strand at 24.058 kb on + strand at 24.066 kb on - strand at 24.066 kb on - strand at 24.109 kb on - strand at 24.463 kb on + strand, within DvMF_0019 at 24.764 kb on + strand, within DvMF_0019 at 24.894 kb on + strand, within DvMF_0019 at 24.979 kb on + strand, within DvMF_0019 at 24.980 kb on - strand, within DvMF_0019 at 25.033 kb on - strand, within DvMF_0019 at 25.049 kb on + strand, within DvMF_0019 at 25.101 kb on + strand, within DvMF_0019 at 25.119 kb on + strand, within DvMF_0019 at 25.127 kb on - strand, within DvMF_0019 at 25.313 kb on + strand at 25.363 kb on - strand at 25.575 kb on + strand at 25.700 kb on - strand, within DvMF_0020 at 25.917 kb on - strand, within DvMF_0020
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.031 % remove 23,304 + DvMF_0017 0.80 -0.6 23,334 + DvMF_0017 0.84 -1.2 23,460 - +0.3 23,723 - DvMF_0018 0.44 -3.8 24,058 + +1.2 24,058 + -1.0 24,066 - -0.5 24,066 - -1.0 24,109 - -3.4 24,463 + DvMF_0019 0.28 -0.7 24,764 + DvMF_0019 0.51 -0.6 24,894 + DvMF_0019 0.60 -1.9 24,979 + DvMF_0019 0.67 -0.4 24,980 - DvMF_0019 0.67 +0.8 25,033 - DvMF_0019 0.71 +0.5 25,049 + DvMF_0019 0.72 -0.9 25,101 + DvMF_0019 0.76 -0.6 25,119 + DvMF_0019 0.77 -2.7 25,127 - DvMF_0019 0.78 -0.3 25,313 + -3.7 25,363 - -0.1 25,575 + -0.8 25,700 - DvMF_0020 0.13 -1.3 25,917 - DvMF_0020 0.33 -2.3
Or see this region's nucleotide sequence