Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1998 and Shew_1999 are separated by 62 nucleotides Shew_1999 and Shew_R0065 are separated by 395 nucleotides Shew_R0065 and Shew_R0066 are separated by 38 nucleotides Shew_R0066 and Shew_R0067 are separated by 35 nucleotides Shew_R0067 and Shew_2000 are separated by 233 nucleotides
Shew_1998: Shew_1998 - protein of unknown function UPF0052 and CofD (RefSeq), at 2,309,364 to 2,310,275
_1998
Shew_1999: Shew_1999 - aromatic amino acid aminotransferase (RefSeq), at 2,310,338 to 2,311,531
_1999
Shew_R0065: Shew_R0065 - tRNA-Leu (RefSeq), at 2,311,927 to 2,312,013
_R0065
Shew_R0066: Shew_R0066 - tRNA-Cys (RefSeq), at 2,312,052 to 2,312,125
_R0066
Shew_R0067: Shew_R0067 - tRNA-Gly (RefSeq), at 2,312,161 to 2,312,236
_R0067
Shew_2000: Shew_2000 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,312,470 to 2,313,906
_2000
Position (kb)
2310
2311
2312 Strain fitness (log2 ratio)
-1
0
1 at 2309.834 kb on - strand, within Shew_1998 at 2310.407 kb on - strand at 2310.849 kb on + strand, within Shew_1999 at 2311.513 kb on - strand at 2311.574 kb on + strand at 2311.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,309,834 - Shew_1998 0.52 +0.4 2,310,407 - -0.3 2,310,849 + Shew_1999 0.43 -0.4 2,311,513 - -0.1 2,311,574 + -0.2 2,311,574 + +0.1
Or see this region's nucleotide sequence