Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0569 and Shew_0570 are separated by 334 nucleotides Shew_0570 and Shew_0571 overlap by 1 nucleotides Shew_0571 and Shew_0572 overlap by 1 nucleotides Shew_0572 and sspA are separated by 108 nucleotides
Shew_0569: Shew_0569 - HflC protein (RefSeq), at 644,958 to 645,836
_0569
Shew_0570: Shew_0570 - ubiquinol-cytochrome c reductase, iron-sulfur subunit (RefSeq), at 646,171 to 646,761
_0570
Shew_0571: Shew_0571 - cytochrome b/b6 domain-containing protein (RefSeq), at 646,761 to 647,975
_0571
Shew_0572: Shew_0572 - cytochrome c1 (RefSeq), at 647,975 to 648,676
_0572
Shew_0573: sspA - stringent starvation protein A (RefSeq), at 648,785 to 649,414
sspA
Position (kb) 646
647
648 Strain fitness (log2 ratio) -1
0
1 at 645.766 kb on + strand at 645.766 kb on + strand at 645.766 kb on + strand at 645.772 kb on + strand at 646.601 kb on - strand, within Shew_0570 at 646.783 kb on + strand at 647.008 kb on - strand, within Shew_0571 at 647.259 kb on - strand, within Shew_0571 at 648.665 kb on + strand at 648.673 kb on - strand at 648.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 645,766 + +0.5 645,766 + +0.3 645,766 + +0.5 645,772 + -0.0 646,601 - Shew_0570 0.73 -0.8 646,783 + -0.5 647,008 - Shew_0571 0.20 -1.0 647,259 - Shew_0571 0.41 -0.7 648,665 + -0.5 648,673 - +0.9 648,673 - -1.0
Or see this region's nucleotide sequence