Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3138 and BT3139 are separated by 275 nucleotides BT3139 and BT3140 are separated by 82 nucleotides BT3140 and BT3141 are separated by 397 nucleotides
BT3138: BT3138 - hypothetical protein (NCBI ptt file), at 4,005,188 to 4,005,547
BT3138
BT3139: BT3139 - conserved hypothetical protein (NCBI ptt file), at 4,005,823 to 4,006,131
BT3139
BT3140: BT3140 - conserved hypothetical protein (NCBI ptt file), at 4,006,214 to 4,006,525
BT3140
BT3141: BT3141 - hypothetical protein (NCBI ptt file), at 4,006,923 to 4,007,405
BT3141
Position (kb)
4006
4007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4005.226 kb on + strand, within BT3138 at 4005.226 kb on + strand, within BT3138 at 4005.226 kb on + strand, within BT3138 at 4005.251 kb on - strand, within BT3138 at 4005.251 kb on - strand, within BT3138 at 4005.253 kb on - strand, within BT3138 at 4005.253 kb on - strand, within BT3138 at 4005.253 kb on - strand, within BT3138 at 4005.257 kb on + strand, within BT3138 at 4005.257 kb on + strand, within BT3138 at 4005.257 kb on + strand, within BT3138 at 4005.257 kb on + strand, within BT3138 at 4005.258 kb on - strand, within BT3138 at 4005.258 kb on - strand, within BT3138 at 4005.264 kb on + strand, within BT3138 at 4005.264 kb on + strand, within BT3138 at 4005.269 kb on + strand, within BT3138 at 4005.269 kb on + strand, within BT3138 at 4005.270 kb on - strand at 4005.273 kb on + strand, within BT3138 at 4005.312 kb on + strand, within BT3138 at 4005.328 kb on - strand, within BT3138 at 4005.348 kb on - strand, within BT3138 at 4005.348 kb on - strand, within BT3138 at 4005.348 kb on - strand, within BT3138 at 4005.371 kb on + strand, within BT3138 at 4005.372 kb on - strand, within BT3138 at 4005.375 kb on - strand, within BT3138 at 4005.375 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.385 kb on - strand, within BT3138 at 4005.395 kb on - strand, within BT3138 at 4005.395 kb on - strand, within BT3138 at 4005.400 kb on - strand, within BT3138 at 4005.403 kb on + strand, within BT3138 at 4005.403 kb on + strand, within BT3138 at 4005.404 kb on - strand, within BT3138 at 4005.425 kb on + strand, within BT3138 at 4005.425 kb on + strand, within BT3138 at 4005.481 kb on + strand, within BT3138 at 4005.495 kb on - strand, within BT3138 at 4005.495 kb on - strand, within BT3138 at 4005.495 kb on - strand at 4005.496 kb on + strand, within BT3138 at 4005.496 kb on + strand, within BT3138 at 4005.497 kb on - strand, within BT3138 at 4005.497 kb on - strand, within BT3138 at 4005.497 kb on - strand, within BT3138 at 4005.497 kb on - strand, within BT3138 at 4005.498 kb on + strand, within BT3138 at 4005.499 kb on - strand, within BT3138 at 4005.513 kb on + strand at 4005.514 kb on - strand at 4005.519 kb on + strand at 4005.538 kb on + strand at 4005.541 kb on + strand at 4005.541 kb on + strand at 4005.541 kb on + strand at 4005.541 kb on + strand at 4005.542 kb on - strand at 4005.542 kb on - strand at 4005.542 kb on - strand at 4005.542 kb on - strand at 4005.545 kb on + strand at 4005.576 kb on - strand at 4005.576 kb on - strand at 4005.582 kb on - strand at 4005.584 kb on - strand at 4005.674 kb on + strand at 4005.676 kb on + strand at 4005.682 kb on + strand at 4005.694 kb on + strand at 4005.729 kb on - strand at 4005.747 kb on - strand at 4005.747 kb on - strand at 4005.749 kb on - strand at 4005.757 kb on + strand at 4005.769 kb on + strand at 4005.771 kb on + strand at 4005.771 kb on + strand at 4005.771 kb on + strand at 4005.772 kb on - strand at 4005.772 kb on - strand at 4005.772 kb on - strand at 4005.815 kb on + strand at 4005.815 kb on + strand at 4005.815 kb on + strand at 4005.815 kb on + strand at 4005.816 kb on - strand at 4005.816 kb on - strand at 4006.032 kb on - strand, within BT3139 at 4006.117 kb on + strand at 4006.131 kb on + strand at 4006.131 kb on + strand at 4006.131 kb on + strand at 4006.132 kb on - strand at 4006.155 kb on - strand at 4006.155 kb on - strand at 4006.205 kb on - strand at 4006.216 kb on - strand at 4006.273 kb on + strand, within BT3140 at 4006.297 kb on - strand, within BT3140 at 4006.297 kb on - strand, within BT3140 at 4006.321 kb on + strand, within BT3140 at 4006.322 kb on - strand, within BT3140 at 4006.488 kb on + strand, within BT3140 at 4006.548 kb on + strand at 4006.551 kb on + strand at 4006.552 kb on - strand at 4006.556 kb on + strand at 4006.557 kb on - strand at 4006.557 kb on - strand at 4006.557 kb on - strand at 4006.580 kb on - strand at 4006.658 kb on - strand at 4006.658 kb on - strand at 4006.725 kb on - strand at 4006.771 kb on - strand at 4006.802 kb on - strand at 4007.007 kb on - strand, within BT3141 at 4007.007 kb on - strand, within BT3141 at 4007.034 kb on - strand, within BT3141 at 4007.040 kb on - strand, within BT3141 at 4007.040 kb on - strand at 4007.059 kb on + strand, within BT3141 at 4007.114 kb on + strand, within BT3141 at 4007.197 kb on + strand, within BT3141 at 4007.449 kb on + strand at 4007.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 4,005,226 + BT3138 0.11 +2.0 4,005,226 + BT3138 0.11 +2.5 4,005,226 + BT3138 0.11 +0.2 4,005,251 - BT3138 0.17 +1.3 4,005,251 - BT3138 0.17 +2.3 4,005,253 - BT3138 0.18 +2.3 4,005,253 - BT3138 0.18 -1.4 4,005,253 - BT3138 0.18 -1.5 4,005,257 + BT3138 0.19 -3.4 4,005,257 + BT3138 0.19 -2.1 4,005,257 + BT3138 0.19 -2.0 4,005,257 + BT3138 0.19 -3.1 4,005,258 - BT3138 0.19 -1.3 4,005,258 - BT3138 0.19 -1.4 4,005,264 + BT3138 0.21 -1.6 4,005,264 + BT3138 0.21 +1.2 4,005,269 + BT3138 0.23 +1.3 4,005,269 + BT3138 0.23 -1.8 4,005,270 - +3.0 4,005,273 + BT3138 0.24 -4.0 4,005,312 + BT3138 0.34 -1.4 4,005,328 - BT3138 0.39 +0.1 4,005,348 - BT3138 0.44 +1.9 4,005,348 - BT3138 0.44 -0.1 4,005,348 - BT3138 0.44 -3.6 4,005,371 + BT3138 0.51 -1.4 4,005,372 - BT3138 0.51 +2.0 4,005,375 - BT3138 0.52 -2.0 4,005,375 - BT3138 0.52 +1.7 4,005,385 - BT3138 0.55 +1.8 4,005,385 - BT3138 0.55 +1.3 4,005,385 - BT3138 0.55 -0.4 4,005,385 - BT3138 0.55 -1.0 4,005,385 - BT3138 0.55 -1.5 4,005,385 - BT3138 0.55 -1.4 4,005,395 - BT3138 0.57 -3.1 4,005,395 - BT3138 0.57 -2.8 4,005,400 - BT3138 0.59 +0.5 4,005,403 + BT3138 0.60 -1.9 4,005,403 + BT3138 0.60 +1.0 4,005,404 - BT3138 0.60 -1.7 4,005,425 + BT3138 0.66 -2.4 4,005,425 + BT3138 0.66 -2.3 4,005,481 + BT3138 0.81 -1.2 4,005,495 - BT3138 0.85 +2.6 4,005,495 - BT3138 0.85 +2.4 4,005,495 - +0.0 4,005,496 + BT3138 0.86 +0.5 4,005,496 + BT3138 0.86 +2.4 4,005,497 - BT3138 0.86 -2.4 4,005,497 - BT3138 0.86 +1.0 4,005,497 - BT3138 0.86 -1.4 4,005,497 - BT3138 0.86 -4.0 4,005,498 + BT3138 0.86 -0.6 4,005,499 - BT3138 0.86 -1.6 4,005,513 + -3.7 4,005,514 - -2.4 4,005,519 + -0.2 4,005,538 + +0.0 4,005,541 + -1.8 4,005,541 + -2.6 4,005,541 + -0.6 4,005,541 + -2.6 4,005,542 - -2.2 4,005,542 - +0.7 4,005,542 - +1.7 4,005,542 - +1.0 4,005,545 + -1.6 4,005,576 - +0.6 4,005,576 - +0.8 4,005,582 - +0.5 4,005,584 - -0.4 4,005,674 + +1.0 4,005,676 + +1.7 4,005,682 + +0.0 4,005,694 + -0.2 4,005,729 - +1.5 4,005,747 - +0.0 4,005,747 - -0.8 4,005,749 - +1.9 4,005,757 + -0.6 4,005,769 + +1.0 4,005,771 + -1.9 4,005,771 + -0.4 4,005,771 + +2.3 4,005,772 - -3.3 4,005,772 - -2.0 4,005,772 - -2.5 4,005,815 + +0.0 4,005,815 + +1.0 4,005,815 + +2.0 4,005,815 + +0.8 4,005,816 - +2.3 4,005,816 - +1.2 4,006,032 - BT3139 0.68 +0.7 4,006,117 + -1.7 4,006,131 + -0.4 4,006,131 + -1.8 4,006,131 + -2.4 4,006,132 - +0.6 4,006,155 - +1.0 4,006,155 - +0.4 4,006,205 - +0.0 4,006,216 - -2.2 4,006,273 + BT3140 0.19 -1.7 4,006,297 - BT3140 0.27 -1.5 4,006,297 - BT3140 0.27 -3.2 4,006,321 + BT3140 0.34 -3.0 4,006,322 - BT3140 0.35 +0.0 4,006,488 + BT3140 0.88 +0.0 4,006,548 + -2.1 4,006,551 + +0.9 4,006,552 - -3.9 4,006,556 + +0.0 4,006,557 - +1.3 4,006,557 - -1.0 4,006,557 - -1.3 4,006,580 - +1.4 4,006,658 - -1.3 4,006,658 - +1.2 4,006,725 - +0.8 4,006,771 - -2.9 4,006,802 - -1.6 4,007,007 - BT3141 0.17 +1.9 4,007,007 - BT3141 0.17 -2.0 4,007,034 - BT3141 0.23 +0.0 4,007,040 - BT3141 0.24 -3.4 4,007,040 - +3.0 4,007,059 + BT3141 0.28 -2.0 4,007,114 + BT3141 0.40 -2.3 4,007,197 + BT3141 0.57 +2.1 4,007,449 + +0.0 4,007,486 - +1.0
Or see this region's nucleotide sequence