Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0232 and BT0233 are separated by 366 nucleotides BT0233 and BT0234 are separated by 128 nucleotides
BT0232: BT0232 - putative protein involved in transposition (NCBI ptt file), at 252,590 to 253,357
BT0232
BT0233: BT0233 - integrase (NCBI ptt file), at 253,724 to 254,845
BT0233
BT0234: BT0234 - putative transposase (NCBI ptt file), at 254,974 to 255,924
BT0234
Position (kb) 253
254
255 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 252.906 kb on - strand, within BT0232 at 252.947 kb on - strand, within BT0232 at 252.965 kb on + strand, within BT0232 at 252.966 kb on - strand, within BT0232 at 253.020 kb on - strand, within BT0232 at 253.265 kb on + strand, within BT0232 at 253.268 kb on - strand, within BT0232 at 253.268 kb on - strand, within BT0232 at 253.274 kb on - strand, within BT0232 at 253.327 kb on + strand at 253.353 kb on - strand at 253.359 kb on - strand at 253.464 kb on + strand at 253.645 kb on + strand at 253.712 kb on - strand at 253.990 kb on + strand, within BT0233 at 253.991 kb on - strand, within BT0233 at 254.033 kb on - strand, within BT0233 at 254.080 kb on - strand, within BT0233 at 254.101 kb on - strand, within BT0233 at 254.109 kb on - strand, within BT0233 at 254.156 kb on + strand, within BT0233 at 254.371 kb on + strand, within BT0233 at 254.389 kb on + strand, within BT0233 at 254.418 kb on + strand, within BT0233 at 254.465 kb on - strand, within BT0233 at 254.465 kb on - strand, within BT0233 at 254.497 kb on + strand, within BT0233 at 254.499 kb on + strand, within BT0233 at 254.500 kb on - strand, within BT0233 at 254.698 kb on - strand, within BT0233 at 254.704 kb on - strand, within BT0233 at 254.704 kb on - strand, within BT0233 at 254.766 kb on + strand at 254.778 kb on + strand at 254.778 kb on + strand at 254.822 kb on + strand at 254.822 kb on + strand at 254.907 kb on + strand at 254.985 kb on + strand at 255.052 kb on + strand at 255.058 kb on - strand at 255.081 kb on + strand, within BT0234 at 255.112 kb on + strand, within BT0234 at 255.113 kb on - strand, within BT0234 at 255.113 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.157 kb on - strand, within BT0234 at 255.201 kb on - strand, within BT0234 at 255.201 kb on - strand, within BT0234 at 255.204 kb on + strand, within BT0234 at 255.204 kb on + strand, within BT0234 at 255.234 kb on + strand, within BT0234 at 255.242 kb on + strand, within BT0234 at 255.277 kb on - strand, within BT0234 at 255.277 kb on - strand, within BT0234 at 255.290 kb on - strand, within BT0234 at 255.357 kb on + strand, within BT0234 at 255.428 kb on + strand, within BT0234 at 255.623 kb on - strand, within BT0234 at 255.703 kb on - strand, within BT0234 at 255.704 kb on + strand, within BT0234 at 255.705 kb on - strand, within BT0234 at 255.725 kb on - strand, within BT0234 at 255.747 kb on + strand, within BT0234 at 255.753 kb on - strand, within BT0234
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 252,906 - BT0232 0.41 -1.6 252,947 - BT0232 0.46 -0.3 252,965 + BT0232 0.49 -2.6 252,966 - BT0232 0.49 -2.6 253,020 - BT0232 0.56 -0.6 253,265 + BT0232 0.88 -1.0 253,268 - BT0232 0.88 -1.8 253,268 - BT0232 0.88 +0.4 253,274 - BT0232 0.89 -0.4 253,327 + -0.3 253,353 - +1.1 253,359 - +0.9 253,464 + -0.4 253,645 + -2.3 253,712 - -1.0 253,990 + BT0233 0.24 -0.2 253,991 - BT0233 0.24 -1.8 254,033 - BT0233 0.28 +0.4 254,080 - BT0233 0.32 +0.6 254,101 - BT0233 0.34 -2.5 254,109 - BT0233 0.34 -2.1 254,156 + BT0233 0.39 -2.9 254,371 + BT0233 0.58 -1.8 254,389 + BT0233 0.59 -3.2 254,418 + BT0233 0.62 +2.4 254,465 - BT0233 0.66 -2.1 254,465 - BT0233 0.66 +0.7 254,497 + BT0233 0.69 -0.8 254,499 + BT0233 0.69 +0.6 254,500 - BT0233 0.69 -2.3 254,698 - BT0233 0.87 -0.8 254,704 - BT0233 0.87 -2.3 254,704 - BT0233 0.87 -0.5 254,766 + +0.4 254,778 + -2.0 254,778 + +1.3 254,822 + +1.1 254,822 + -2.4 254,907 + -3.8 254,985 + +1.0 255,052 + -2.4 255,058 - +2.5 255,081 + BT0234 0.11 -2.0 255,112 + BT0234 0.15 -1.7 255,113 - BT0234 0.15 -1.2 255,113 - BT0234 0.15 -0.1 255,141 - BT0234 0.18 -0.5 255,141 - BT0234 0.18 -2.5 255,141 - BT0234 0.18 -0.5 255,157 - BT0234 0.19 -2.6 255,201 - BT0234 0.24 -1.8 255,201 - BT0234 0.24 +0.6 255,204 + BT0234 0.24 +0.2 255,204 + BT0234 0.24 +2.3 255,234 + BT0234 0.27 -1.0 255,242 + BT0234 0.28 +0.5 255,277 - BT0234 0.32 +1.3 255,277 - BT0234 0.32 -2.5 255,290 - BT0234 0.33 +0.4 255,357 + BT0234 0.40 -3.2 255,428 + BT0234 0.48 -2.7 255,623 - BT0234 0.68 -2.8 255,703 - BT0234 0.77 -1.5 255,704 + BT0234 0.77 -3.1 255,705 - BT0234 0.77 -3.6 255,725 - BT0234 0.79 +0.9 255,747 + BT0234 0.81 +2.5 255,753 - BT0234 0.82 +0.2
Or see this region's nucleotide sequence