Experiment: MoLS4 with L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1082 and phoB are separated by 173 nucleotides phoB and pstB-1 are separated by 27 nucleotides pstB-1 and phoU are separated by 8 nucleotides
DVU1082: DVU1082 - 3- 5 exonuclease domain protein (TIGR), at 1,186,345 to 1,186,953
DVU1082
DVU1083: phoB - phosphate regulon transcriptional regulator PhoB (TIGR), at 1,187,127 to 1,187,816
phoB
DVU1084: pstB-1 - phosphate ABC transporter, ATP-binding protein (TIGR), at 1,187,844 to 1,188,611
pstB-1
DVU1085: phoU - phosphate transport system protein PhoU (TIGR), at 1,188,620 to 1,189,285
phoU
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-1
0
1
2 at 1186.858 kb on - strand, within DVU1082 at 1187.052 kb on + strand at 1187.064 kb on + strand at 1187.118 kb on + strand at 1187.873 kb on + strand at 1187.923 kb on - strand, within pstB-1 at 1188.001 kb on + strand, within pstB-1 at 1188.071 kb on + strand, within pstB-1 at 1188.134 kb on + strand, within pstB-1 at 1188.183 kb on + strand, within pstB-1 at 1188.201 kb on - strand, within pstB-1 at 1188.228 kb on + strand, within pstB-1 at 1188.251 kb on + strand, within pstB-1 at 1188.257 kb on - strand, within pstB-1 at 1188.259 kb on - strand, within pstB-1 at 1188.276 kb on + strand, within pstB-1 at 1188.276 kb on + strand, within pstB-1 at 1188.284 kb on - strand, within pstB-1 at 1188.320 kb on - strand, within pstB-1 at 1188.370 kb on - strand, within pstB-1 at 1188.517 kb on - strand, within pstB-1 at 1188.548 kb on + strand at 1188.572 kb on + strand at 1188.595 kb on - strand at 1188.716 kb on + strand, within phoU at 1188.807 kb on + strand, within phoU at 1188.867 kb on + strand, within phoU at 1188.875 kb on - strand, within phoU at 1188.980 kb on - strand, within phoU at 1188.981 kb on + strand, within phoU at 1189.016 kb on + strand, within phoU at 1189.024 kb on - strand, within phoU at 1189.193 kb on - strand, within phoU at 1189.280 kb on + strand at 1189.336 kb on - strand at 1189.390 kb on - strand at 1189.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan (N) remove 1,186,858 - DVU1082 0.84 +0.1 1,187,052 + -0.0 1,187,064 + -0.5 1,187,118 + +0.5 1,187,873 + +0.3 1,187,923 - pstB-1 DVU1084 0.10 +2.0 1,188,001 + pstB-1 DVU1084 0.20 +0.2 1,188,071 + pstB-1 DVU1084 0.30 -0.4 1,188,134 + pstB-1 DVU1084 0.38 +0.1 1,188,183 + pstB-1 DVU1084 0.44 +0.1 1,188,201 - pstB-1 DVU1084 0.46 +0.9 1,188,228 + pstB-1 DVU1084 0.50 -0.1 1,188,251 + pstB-1 DVU1084 0.53 +0.1 1,188,257 - pstB-1 DVU1084 0.54 +0.4 1,188,259 - pstB-1 DVU1084 0.54 +0.5 1,188,276 + pstB-1 DVU1084 0.56 +0.0 1,188,276 + pstB-1 DVU1084 0.56 -0.2 1,188,284 - pstB-1 DVU1084 0.57 -0.5 1,188,320 - pstB-1 DVU1084 0.62 +0.5 1,188,370 - pstB-1 DVU1084 0.68 +0.2 1,188,517 - pstB-1 DVU1084 0.88 +0.6 1,188,548 + +0.1 1,188,572 + +0.1 1,188,595 - +0.2 1,188,716 + phoU DVU1085 0.14 +0.1 1,188,807 + phoU DVU1085 0.28 +0.1 1,188,867 + phoU DVU1085 0.37 -0.4 1,188,875 - phoU DVU1085 0.38 +0.4 1,188,980 - phoU DVU1085 0.54 -0.6 1,188,981 + phoU DVU1085 0.54 -1.4 1,189,016 + phoU DVU1085 0.59 +0.2 1,189,024 - phoU DVU1085 0.61 -0.1 1,189,193 - phoU DVU1085 0.86 -0.3 1,189,280 + +0.1 1,189,336 - -0.3 1,189,390 - -0.2 1,189,514 - -0.4
Or see this region's nucleotide sequence