Experiment: H2 deepwell acetate FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07725 and MMP_RS07730 are separated by 23 nucleotides MMP_RS07730 and MMP_RS07735 are separated by 16 nucleotides MMP_RS07735 and MMP_RS07740 are separated by 21 nucleotides MMP_RS07740 and MMP_RS07745 are separated by 14 nucleotides
MMP_RS07725: MMP_RS07725 - 4Fe-4S dicluster domain-containing protein, at 1,462,970 to 1,463,473
_RS07725
MMP_RS07730: MMP_RS07730 - pyruvate synthase subunit beta, at 1,463,497 to 1,464,393
_RS07730
MMP_RS07735: MMP_RS07735 - 2-ketoisovalerate ferredoxin oxidoreductase subunit alpha, at 1,464,410 to 1,465,573
_RS07735
MMP_RS07740: MMP_RS07740 - 4Fe-4S binding protein, at 1,465,595 to 1,465,852
_RS07740
MMP_RS07745: MMP_RS07745 - pyruvate ferredoxin oxidoreductase subunit gamma, at 1,465,867 to 1,466,397
_RS07745
Position (kb) 1464
1465
1466 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1463.740 kb on + strand, within MMP_RS07730 at 1463.848 kb on - strand, within MMP_RS07730 at 1463.892 kb on - strand, within MMP_RS07730 at 1463.893 kb on + strand, within MMP_RS07730 at 1464.006 kb on - strand, within MMP_RS07730 at 1464.393 kb on - strand at 1464.518 kb on - strand at 1464.773 kb on - strand, within MMP_RS07735 at 1464.993 kb on - strand, within MMP_RS07735 at 1465.827 kb on - strand at 1466.148 kb on - strand at 1466.189 kb on + strand at 1466.474 kb on + strand at 1466.534 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 deepwell acetate FREE remove 1,463,740 + MMP_RS07730 0.27 +0.9 1,463,848 - MMP_RS07730 0.39 -3.3 1,463,892 - MMP_RS07730 0.44 -0.9 1,463,893 + MMP_RS07730 0.44 -2.7 1,464,006 - MMP_RS07730 0.57 -2.0 1,464,393 - -0.1 1,464,518 - -2.6 1,464,773 - MMP_RS07735 0.31 -2.1 1,464,993 - MMP_RS07735 0.50 -1.8 1,465,827 - -2.1 1,466,148 - -2.2 1,466,189 + -0.9 1,466,474 + -1.7 1,466,534 + +2.4
Or see this region's nucleotide sequence