Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS18700

Experiment: Recovery broth with Bicyclomycin 0.07 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS18690 and BBR_RS18695 are separated by 1 nucleotidesBBR_RS18695 and BBR_RS18700 are separated by 51 nucleotidesBBR_RS18700 and BBR_RS18705 are separated by 495 nucleotides BBR_RS18690: BBR_RS18690 - diacylglycerol kinase, at 1,981,209 to 1,982,390 _RS18690 BBR_RS18695: BBR_RS18695 - PRD domain-containing protein, at 1,982,392 to 1,983,285 _RS18695 BBR_RS18700: BBR_RS18700 - PTS beta-glucoside transporter subunit EIIBCA, at 1,983,337 to 1,985,427 _RS18700 BBR_RS18705: BBR_RS18705 - alpha-D-glucose phosphate-specific phosphoglucomutase, at 1,985,923 to 1,987,599 _RS18705 Position (kb) 1983 1984 1985 1986Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8 9at 1982.452 kb on + strandat 1982.589 kb on - strand, within BBR_RS18695at 1982.615 kb on + strand, within BBR_RS18695at 1982.623 kb on - strand, within BBR_RS18695at 1982.828 kb on + strand, within BBR_RS18695at 1982.838 kb on + strand, within BBR_RS18695at 1982.882 kb on + strand, within BBR_RS18695at 1982.911 kb on - strand, within BBR_RS18695at 1982.981 kb on - strand, within BBR_RS18695at 1983.030 kb on + strand, within BBR_RS18695at 1983.130 kb on + strand, within BBR_RS18695at 1983.131 kb on - strand, within BBR_RS18695at 1983.138 kb on - strand, within BBR_RS18695at 1983.143 kb on - strand, within BBR_RS18695at 1983.191 kb on + strand, within BBR_RS18695at 1983.327 kb on - strandat 1983.327 kb on - strandat 1983.335 kb on - strandat 1983.338 kb on - strandat 1983.357 kb on - strandat 1983.418 kb on + strandat 1983.479 kb on + strandat 1983.493 kb on - strandat 1983.523 kb on - strandat 1983.523 kb on - strandat 1983.660 kb on - strand, within BBR_RS18700at 1983.700 kb on - strand, within BBR_RS18700at 1983.710 kb on - strand, within BBR_RS18700at 1983.741 kb on + strand, within BBR_RS18700at 1983.741 kb on + strand, within BBR_RS18700at 1983.749 kb on - strand, within BBR_RS18700at 1983.749 kb on - strand, within BBR_RS18700at 1983.880 kb on - strand, within BBR_RS18700at 1983.906 kb on - strand, within BBR_RS18700at 1984.157 kb on - strand, within BBR_RS18700at 1984.194 kb on + strand, within BBR_RS18700at 1984.364 kb on + strand, within BBR_RS18700at 1984.402 kb on + strand, within BBR_RS18700at 1984.402 kb on + strand, within BBR_RS18700at 1984.407 kb on + strand, within BBR_RS18700at 1984.456 kb on + strand, within BBR_RS18700at 1984.496 kb on - strand, within BBR_RS18700at 1984.635 kb on - strand, within BBR_RS18700at 1984.679 kb on - strand, within BBR_RS18700at 1984.711 kb on + strand, within BBR_RS18700at 1984.730 kb on + strand, within BBR_RS18700at 1984.786 kb on - strand, within BBR_RS18700at 1984.792 kb on - strand, within BBR_RS18700at 1984.830 kb on - strand, within BBR_RS18700at 1984.861 kb on - strand, within BBR_RS18700at 1984.986 kb on + strand, within BBR_RS18700at 1984.993 kb on - strand, within BBR_RS18700at 1985.037 kb on + strand, within BBR_RS18700at 1985.122 kb on - strand, within BBR_RS18700at 1985.122 kb on - strand, within BBR_RS18700at 1985.122 kb on - strand, within BBR_RS18700at 1985.147 kb on - strand, within BBR_RS18700at 1985.205 kb on + strand, within BBR_RS18700at 1985.235 kb on + strandat 1985.249 kb on - strandat 1985.326 kb on - strandat 1985.382 kb on + strandat 1985.425 kb on - strandat 1985.451 kb on + strandat 1985.456 kb on + strandat 1985.456 kb on + strandat 1985.464 kb on - strandat 1985.464 kb on - strandat 1985.464 kb on - strandat 1985.469 kb on - strandat 1985.484 kb on + strandat 1985.484 kb on + strandat 1985.537 kb on + strandat 1985.550 kb on - strandat 1985.564 kb on - strandat 1985.573 kb on - strandat 1985.592 kb on - strandat 1985.618 kb on - strandat 1985.683 kb on - strandat 1985.726 kb on - strandat 1985.795 kb on + strandat 1985.808 kb on + strandat 1985.811 kb on - strandat 1985.839 kb on + strandat 1985.898 kb on + strandat 1985.921 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Bicyclomycin 0.07 mM
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1,982,452 + +8.8
1,982,589 - BBR_RS18695 0.22 +9.2
1,982,615 + BBR_RS18695 0.25 +8.9
1,982,623 - BBR_RS18695 0.26 +8.9
1,982,828 + BBR_RS18695 0.49 +9.0
1,982,838 + BBR_RS18695 0.50 +8.9
1,982,882 + BBR_RS18695 0.55 +8.4
1,982,911 - BBR_RS18695 0.58 +8.6
1,982,981 - BBR_RS18695 0.66 +9.1
1,983,030 + BBR_RS18695 0.71 +8.7
1,983,130 + BBR_RS18695 0.83 +8.6
1,983,131 - BBR_RS18695 0.83 +8.8
1,983,138 - BBR_RS18695 0.83 +8.8
1,983,143 - BBR_RS18695 0.84 +8.8
1,983,191 + BBR_RS18695 0.89 +9.0
1,983,327 - -1.1
1,983,327 - -0.3
1,983,335 - +2.9
1,983,338 - +0.4
1,983,357 - +5.0
1,983,418 + +7.7
1,983,479 + +8.2
1,983,493 - +7.9
1,983,523 - +8.0
1,983,523 - +7.8
1,983,660 - BBR_RS18700 0.15 +8.0
1,983,700 - BBR_RS18700 0.17 +7.4
1,983,710 - BBR_RS18700 0.18 +7.8
1,983,741 + BBR_RS18700 0.19 +7.9
1,983,741 + BBR_RS18700 0.19 +7.6
1,983,749 - BBR_RS18700 0.20 +7.8
1,983,749 - BBR_RS18700 0.20 +8.1
1,983,880 - BBR_RS18700 0.26 +8.0
1,983,906 - BBR_RS18700 0.27 +8.1
1,984,157 - BBR_RS18700 0.39 +7.6
1,984,194 + BBR_RS18700 0.41 +7.7
1,984,364 + BBR_RS18700 0.49 +7.4
1,984,402 + BBR_RS18700 0.51 +8.5
1,984,402 + BBR_RS18700 0.51 +8.3
1,984,407 + BBR_RS18700 0.51 +8.2
1,984,456 + BBR_RS18700 0.54 +7.9
1,984,496 - BBR_RS18700 0.55 +8.6
1,984,635 - BBR_RS18700 0.62 +8.8
1,984,679 - BBR_RS18700 0.64 +8.1
1,984,711 + BBR_RS18700 0.66 +8.0
1,984,730 + BBR_RS18700 0.67 +7.7
1,984,786 - BBR_RS18700 0.69 +8.0
1,984,792 - BBR_RS18700 0.70 +8.8
1,984,830 - BBR_RS18700 0.71 +8.5
1,984,861 - BBR_RS18700 0.73 +8.3
1,984,986 + BBR_RS18700 0.79 +8.9
1,984,993 - BBR_RS18700 0.79 +8.3
1,985,037 + BBR_RS18700 0.81 +8.5
1,985,122 - BBR_RS18700 0.85 +8.5
1,985,122 - BBR_RS18700 0.85 +8.4
1,985,122 - BBR_RS18700 0.85 +8.7
1,985,147 - BBR_RS18700 0.87 +8.7
1,985,205 + BBR_RS18700 0.89 +8.5
1,985,235 + +8.6
1,985,249 - +8.6
1,985,326 - +8.6
1,985,382 + +8.7
1,985,425 - +8.7
1,985,451 + +8.8
1,985,456 + +8.8
1,985,456 + +8.8
1,985,464 - +7.7
1,985,464 - +4.2
1,985,464 - +8.7
1,985,469 - +4.4
1,985,484 + +9.1
1,985,484 + +8.7
1,985,537 + +8.8
1,985,550 - +7.9
1,985,564 - +9.4
1,985,573 - +8.6
1,985,592 - +8.3
1,985,618 - +8.9
1,985,683 - +6.4
1,985,726 - +3.4
1,985,795 + -0.6
1,985,808 + +0.7
1,985,811 - +0.8
1,985,839 + +3.3
1,985,898 + +1.6
1,985,921 - +0.6

Or see this region's nucleotide sequence