Experiment: Recovery broth with Bicyclomycin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14220 and BBR_RS14225 are separated by 71 nucleotides BBR_RS14225 and BBR_RS14230 are separated by 57 nucleotides
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
BBR_RS14225: BBR_RS14225 - ComF family protein, at 966,484 to 967,158
_RS14225
BBR_RS14230: BBR_RS14230 - HAMP domain-containing histidine kinase, at 967,216 to 968,289
_RS14230
Position (kb) 966
967
968 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 965.503 kb on + strand at 965.570 kb on - strand at 965.589 kb on - strand at 965.620 kb on + strand at 965.673 kb on + strand at 965.678 kb on + strand at 965.681 kb on - strand at 965.700 kb on + strand, within BBR_RS14220 at 965.700 kb on + strand, within BBR_RS14220 at 965.703 kb on - strand, within BBR_RS14220 at 965.708 kb on - strand, within BBR_RS14220 at 965.710 kb on + strand, within BBR_RS14220 at 965.786 kb on + strand, within BBR_RS14220 at 965.810 kb on - strand, within BBR_RS14220 at 965.834 kb on - strand, within BBR_RS14220 at 965.862 kb on - strand, within BBR_RS14220 at 965.865 kb on + strand, within BBR_RS14220 at 965.883 kb on + strand, within BBR_RS14220 at 965.891 kb on + strand, within BBR_RS14220 at 965.897 kb on - strand, within BBR_RS14220 at 965.919 kb on - strand, within BBR_RS14220 at 965.961 kb on + strand, within BBR_RS14220 at 965.984 kb on - strand, within BBR_RS14220 at 966.096 kb on - strand, within BBR_RS14220 at 966.113 kb on - strand, within BBR_RS14220 at 966.215 kb on + strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.234 kb on - strand, within BBR_RS14220 at 966.281 kb on + strand, within BBR_RS14220 at 966.299 kb on - strand, within BBR_RS14220 at 966.367 kb on + strand at 966.367 kb on + strand at 966.386 kb on - strand at 966.391 kb on + strand at 966.409 kb on - strand at 966.509 kb on - strand at 966.528 kb on - strand at 966.530 kb on + strand at 966.546 kb on - strand at 966.546 kb on - strand at 966.551 kb on - strand at 966.551 kb on - strand at 966.627 kb on + strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.732 kb on - strand, within BBR_RS14225 at 966.830 kb on + strand, within BBR_RS14225 at 966.833 kb on - strand, within BBR_RS14225 at 966.840 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.926 kb on - strand, within BBR_RS14225 at 967.025 kb on + strand, within BBR_RS14225 at 967.039 kb on - strand, within BBR_RS14225 at 967.053 kb on + strand, within BBR_RS14225 at 967.064 kb on + strand, within BBR_RS14225 at 967.074 kb on + strand, within BBR_RS14225 at 967.106 kb on + strand at 967.208 kb on + strand at 967.278 kb on + strand at 967.284 kb on + strand at 967.332 kb on + strand, within BBR_RS14230 at 967.340 kb on - strand, within BBR_RS14230 at 967.360 kb on + strand, within BBR_RS14230 at 967.365 kb on + strand, within BBR_RS14230 at 967.479 kb on - strand, within BBR_RS14230 at 967.503 kb on + strand, within BBR_RS14230 at 967.509 kb on - strand, within BBR_RS14230 at 967.541 kb on + strand, within BBR_RS14230 at 967.608 kb on - strand, within BBR_RS14230 at 967.609 kb on + strand, within BBR_RS14230 at 967.681 kb on - strand, within BBR_RS14230 at 967.698 kb on - strand, within BBR_RS14230 at 967.707 kb on - strand, within BBR_RS14230 at 967.715 kb on + strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.830 kb on - strand, within BBR_RS14230 at 967.863 kb on + strand, within BBR_RS14230 at 967.915 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 968.005 kb on - strand, within BBR_RS14230 at 968.014 kb on - strand, within BBR_RS14230 at 968.043 kb on - strand, within BBR_RS14230 at 968.102 kb on - strand, within BBR_RS14230 at 968.117 kb on + strand, within BBR_RS14230
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Bicyclomycin 0.07 mM remove 965,503 + +0.5 965,570 - -0.2 965,589 - -0.3 965,620 + +0.2 965,673 + +1.7 965,678 + +0.6 965,681 - +1.3 965,700 + BBR_RS14220 0.11 +0.9 965,700 + BBR_RS14220 0.11 -1.1 965,703 - BBR_RS14220 0.12 +0.1 965,708 - BBR_RS14220 0.12 +0.6 965,710 + BBR_RS14220 0.13 +0.5 965,786 + BBR_RS14220 0.22 +0.8 965,810 - BBR_RS14220 0.25 +0.7 965,834 - BBR_RS14220 0.28 +1.7 965,862 - BBR_RS14220 0.31 +0.2 965,865 + BBR_RS14220 0.32 +1.5 965,883 + BBR_RS14220 0.34 -0.1 965,891 + BBR_RS14220 0.35 +0.8 965,897 - BBR_RS14220 0.36 -0.2 965,919 - BBR_RS14220 0.39 -0.8 965,961 + BBR_RS14220 0.44 -0.3 965,984 - BBR_RS14220 0.47 +0.5 966,096 - BBR_RS14220 0.61 -0.3 966,113 - BBR_RS14220 0.63 +0.7 966,215 + BBR_RS14220 0.75 -0.8 966,222 - BBR_RS14220 0.76 +1.1 966,222 - BBR_RS14220 0.76 +1.2 966,222 - BBR_RS14220 0.76 -0.6 966,234 - BBR_RS14220 0.78 +1.6 966,281 + BBR_RS14220 0.84 +0.5 966,299 - BBR_RS14220 0.86 +0.4 966,367 + -0.8 966,367 + -0.9 966,386 - -0.7 966,391 + -0.3 966,409 - +2.4 966,509 - +0.9 966,528 - +0.7 966,530 + +1.1 966,546 - +0.4 966,546 - +1.7 966,551 - +0.6 966,551 - -1.4 966,627 + BBR_RS14225 0.21 +1.2 966,635 - BBR_RS14225 0.22 -1.2 966,635 - BBR_RS14225 0.22 -0.4 966,732 - BBR_RS14225 0.37 +0.6 966,830 + BBR_RS14225 0.51 -0.2 966,833 - BBR_RS14225 0.52 +1.9 966,840 - BBR_RS14225 0.53 -0.1 966,863 - BBR_RS14225 0.56 -1.0 966,863 - BBR_RS14225 0.56 +0.4 966,926 - BBR_RS14225 0.65 -0.6 967,025 + BBR_RS14225 0.80 -0.7 967,039 - BBR_RS14225 0.82 +1.4 967,053 + BBR_RS14225 0.84 -1.1 967,064 + BBR_RS14225 0.86 -0.2 967,074 + BBR_RS14225 0.87 -1.0 967,106 + +0.1 967,208 + +0.0 967,278 + +2.2 967,284 + +4.5 967,332 + BBR_RS14230 0.11 +0.8 967,340 - BBR_RS14230 0.12 +1.4 967,360 + BBR_RS14230 0.13 +3.9 967,365 + BBR_RS14230 0.14 +1.9 967,479 - BBR_RS14230 0.24 +4.5 967,503 + BBR_RS14230 0.27 +0.6 967,509 - BBR_RS14230 0.27 +1.1 967,541 + BBR_RS14230 0.30 +0.8 967,608 - BBR_RS14230 0.36 +2.2 967,609 + BBR_RS14230 0.37 +2.9 967,681 - BBR_RS14230 0.43 +1.7 967,698 - BBR_RS14230 0.45 +1.4 967,707 - BBR_RS14230 0.46 +3.5 967,715 + BBR_RS14230 0.46 +1.2 967,763 - BBR_RS14230 0.51 +2.9 967,763 - BBR_RS14230 0.51 +4.5 967,830 - BBR_RS14230 0.57 +3.5 967,863 + BBR_RS14230 0.60 +1.7 967,915 - BBR_RS14230 0.65 -1.6 967,919 - BBR_RS14230 0.65 +3.5 967,919 - BBR_RS14230 0.65 +3.5 967,920 - BBR_RS14230 0.66 +0.4 967,920 - BBR_RS14230 0.66 -0.3 968,005 - BBR_RS14230 0.73 -0.0 968,014 - BBR_RS14230 0.74 +0.8 968,043 - BBR_RS14230 0.77 +3.5 968,102 - BBR_RS14230 0.82 +3.5 968,117 + BBR_RS14230 0.84 +2.2
Or see this region's nucleotide sequence