Experiment: Recovery broth with Bicyclomycin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13265 and BBR_RS20750 are separated by 137 nucleotides BBR_RS20750 and BBR_RS20755 are separated by 1 nucleotides BBR_RS20755 and BBR_RS13270 are separated by 131 nucleotides BBR_RS13270 and BBR_RS13275 are separated by 11 nucleotides
BBR_RS13265: BBR_RS13265 - peptide methionine sulfoxide reductase msrA/msrB, at 750,416 to 750,907
_RS13265
BBR_RS20750: BBR_RS20750 - GntR family transcriptional regulator, at 751,045 to 751,170
_RS20750
BBR_RS20755: BBR_RS20755 - IS3 family transposase, at 751,172 to 751,470
_RS20755
BBR_RS13270: BBR_RS13270 - ABC transporter ATP-binding protein, at 751,602 to 752,414
_RS13270
BBR_RS13275: BBR_RS13275 - hypothetical protein, at 752,426 to 753,721
_RS13275
Position (kb)
751
752
753 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 750.619 kb on - strand, within BBR_RS13265 at 750.652 kb on + strand, within BBR_RS13265 at 750.678 kb on + strand, within BBR_RS13265 at 750.678 kb on + strand, within BBR_RS13265 at 750.705 kb on - strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.715 kb on + strand, within BBR_RS13265 at 750.716 kb on - strand, within BBR_RS13265 at 750.721 kb on - strand, within BBR_RS13265 at 750.786 kb on + strand, within BBR_RS13265 at 750.794 kb on - strand, within BBR_RS13265 at 750.814 kb on + strand, within BBR_RS13265 at 750.821 kb on + strand, within BBR_RS13265 at 750.837 kb on - strand, within BBR_RS13265 at 750.840 kb on - strand, within BBR_RS13265 at 750.847 kb on - strand, within BBR_RS13265 at 750.847 kb on - strand, within BBR_RS13265 at 750.854 kb on + strand, within BBR_RS13265 at 750.854 kb on + strand, within BBR_RS13265 at 750.902 kb on + strand at 750.902 kb on + strand at 750.910 kb on - strand at 750.910 kb on - strand at 750.921 kb on - strand at 750.931 kb on - strand at 750.973 kb on + strand at 750.981 kb on - strand at 750.981 kb on - strand at 750.981 kb on - strand at 750.982 kb on - strand at 751.088 kb on + strand, within BBR_RS20750 at 751.108 kb on - strand, within BBR_RS20750 at 751.108 kb on - strand, within BBR_RS20750 at 751.160 kb on + strand at 751.160 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.170 kb on - strand at 751.212 kb on - strand, within BBR_RS20755 at 751.217 kb on + strand, within BBR_RS20755 at 751.217 kb on + strand, within BBR_RS20755 at 751.225 kb on - strand, within BBR_RS20755 at 751.225 kb on - strand, within BBR_RS20755 at 751.230 kb on + strand, within BBR_RS20755 at 751.230 kb on + strand, within BBR_RS20755 at 751.238 kb on - strand, within BBR_RS20755 at 751.293 kb on - strand, within BBR_RS20755 at 751.345 kb on - strand, within BBR_RS20755 at 751.365 kb on + strand, within BBR_RS20755 at 751.373 kb on - strand, within BBR_RS20755 at 751.413 kb on + strand, within BBR_RS20755 at 751.413 kb on - strand, within BBR_RS20755 at 751.413 kb on - strand, within BBR_RS20755 at 751.490 kb on + strand at 751.490 kb on + strand at 751.490 kb on + strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.519 kb on + strand at 751.596 kb on - strand at 751.597 kb on + strand at 751.624 kb on + strand at 751.671 kb on + strand at 751.679 kb on - strand at 751.689 kb on - strand, within BBR_RS13270 at 751.714 kb on + strand, within BBR_RS13270 at 751.742 kb on - strand, within BBR_RS13270 at 751.744 kb on + strand, within BBR_RS13270 at 751.752 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.761 kb on + strand, within BBR_RS13270 at 751.768 kb on + strand, within BBR_RS13270 at 751.771 kb on + strand, within BBR_RS13270 at 751.775 kb on + strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.843 kb on - strand, within BBR_RS13270 at 751.881 kb on + strand, within BBR_RS13270 at 751.901 kb on - strand, within BBR_RS13270 at 751.951 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 752.055 kb on - strand, within BBR_RS13270 at 752.055 kb on - strand, within BBR_RS13270 at 752.097 kb on - strand, within BBR_RS13270 at 752.107 kb on + strand, within BBR_RS13270 at 752.138 kb on + strand, within BBR_RS13270 at 752.162 kb on + strand, within BBR_RS13270 at 752.170 kb on - strand, within BBR_RS13270 at 752.190 kb on - strand, within BBR_RS13270 at 752.209 kb on + strand, within BBR_RS13270 at 752.217 kb on - strand, within BBR_RS13270 at 752.217 kb on - strand, within BBR_RS13270 at 752.271 kb on - strand, within BBR_RS13270 at 752.301 kb on + strand, within BBR_RS13270 at 752.307 kb on - strand, within BBR_RS13270 at 752.361 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.388 kb on + strand at 752.389 kb on - strand at 752.408 kb on + strand at 752.408 kb on + strand at 752.468 kb on - strand at 752.492 kb on - strand at 752.517 kb on - strand at 752.556 kb on + strand, within BBR_RS13275 at 752.564 kb on - strand, within BBR_RS13275 at 752.605 kb on - strand, within BBR_RS13275 at 752.626 kb on - strand, within BBR_RS13275 at 752.663 kb on + strand, within BBR_RS13275 at 752.669 kb on + strand, within BBR_RS13275 at 752.783 kb on + strand, within BBR_RS13275 at 752.790 kb on - strand, within BBR_RS13275 at 752.811 kb on - strand, within BBR_RS13275 at 752.813 kb on + strand, within BBR_RS13275 at 752.813 kb on - strand, within BBR_RS13275 at 752.821 kb on - strand, within BBR_RS13275 at 752.844 kb on - strand, within BBR_RS13275 at 752.850 kb on - strand, within BBR_RS13275 at 752.852 kb on + strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.984 kb on + strand, within BBR_RS13275 at 752.999 kb on + strand, within BBR_RS13275 at 753.022 kb on + strand, within BBR_RS13275 at 753.037 kb on + strand, within BBR_RS13275 at 753.045 kb on - strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.097 kb on + strand, within BBR_RS13275 at 753.105 kb on + strand, within BBR_RS13275 at 753.109 kb on + strand, within BBR_RS13275 at 753.157 kb on - strand, within BBR_RS13275 at 753.194 kb on + strand, within BBR_RS13275 at 753.200 kb on - strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.212 kb on - strand, within BBR_RS13275 at 753.216 kb on + strand, within BBR_RS13275 at 753.226 kb on + strand, within BBR_RS13275 at 753.249 kb on - strand, within BBR_RS13275 at 753.292 kb on - strand, within BBR_RS13275 at 753.294 kb on - strand, within BBR_RS13275 at 753.350 kb on - strand, within BBR_RS13275 at 753.375 kb on + strand, within BBR_RS13275
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Bicyclomycin 0.07 mM remove 750,619 - BBR_RS13265 0.41 +0.8 750,652 + BBR_RS13265 0.48 +0.1 750,678 + BBR_RS13265 0.53 +1.5 750,678 + BBR_RS13265 0.53 -1.0 750,705 - BBR_RS13265 0.59 +0.6 750,713 + BBR_RS13265 0.60 -0.7 750,713 + BBR_RS13265 0.60 +0.4 750,713 + BBR_RS13265 0.60 -0.6 750,713 + BBR_RS13265 0.60 -1.0 750,715 + BBR_RS13265 0.61 +1.4 750,716 - BBR_RS13265 0.61 -0.4 750,721 - BBR_RS13265 0.62 -1.4 750,786 + BBR_RS13265 0.75 +1.0 750,794 - BBR_RS13265 0.77 -2.6 750,814 + BBR_RS13265 0.81 +0.9 750,821 + BBR_RS13265 0.82 +0.5 750,837 - BBR_RS13265 0.86 +0.1 750,840 - BBR_RS13265 0.86 -0.0 750,847 - BBR_RS13265 0.88 +0.7 750,847 - BBR_RS13265 0.88 +0.2 750,854 + BBR_RS13265 0.89 -0.9 750,854 + BBR_RS13265 0.89 -0.5 750,902 + -1.0 750,902 + +1.0 750,910 - +0.7 750,910 - +0.3 750,921 - -1.0 750,931 - +0.6 750,973 + +0.7 750,981 - -1.0 750,981 - -0.0 750,981 - +1.5 750,982 - +0.3 751,088 + BBR_RS20750 0.34 -1.2 751,108 - BBR_RS20750 0.50 +0.7 751,108 - BBR_RS20750 0.50 -0.2 751,160 + +0.6 751,160 + +0.8 751,162 + +1.4 751,162 + -0.3 751,162 + -0.1 751,162 + -1.1 751,170 - +0.7 751,212 - BBR_RS20755 0.13 -0.2 751,217 + BBR_RS20755 0.15 -0.8 751,217 + BBR_RS20755 0.15 -0.4 751,225 - BBR_RS20755 0.18 +0.1 751,225 - BBR_RS20755 0.18 +0.2 751,230 + BBR_RS20755 0.19 +0.1 751,230 + BBR_RS20755 0.19 +0.1 751,238 - BBR_RS20755 0.22 -0.2 751,293 - BBR_RS20755 0.40 +1.7 751,345 - BBR_RS20755 0.58 -0.6 751,365 + BBR_RS20755 0.65 -1.0 751,373 - BBR_RS20755 0.67 -0.2 751,413 + BBR_RS20755 0.81 -0.1 751,413 - BBR_RS20755 0.81 -0.6 751,413 - BBR_RS20755 0.81 +2.4 751,490 + -0.8 751,490 + +0.7 751,490 + -0.5 751,498 - -0.4 751,498 - +1.0 751,498 - +0.2 751,498 - +1.2 751,498 - +0.3 751,498 - +0.4 751,519 + +0.1 751,596 - +0.7 751,597 + +1.8 751,624 + -0.7 751,671 + +0.5 751,679 - +0.0 751,689 - BBR_RS13270 0.11 -1.2 751,714 + BBR_RS13270 0.14 -1.0 751,742 - BBR_RS13270 0.17 +1.0 751,744 + BBR_RS13270 0.17 +2.3 751,752 + BBR_RS13270 0.18 +1.6 751,754 + BBR_RS13270 0.19 -0.1 751,754 + BBR_RS13270 0.19 -1.2 751,754 + BBR_RS13270 0.19 -1.6 751,761 + BBR_RS13270 0.20 +0.3 751,768 + BBR_RS13270 0.20 +0.9 751,771 + BBR_RS13270 0.21 -1.4 751,775 + BBR_RS13270 0.21 -0.6 751,833 - BBR_RS13270 0.28 -0.5 751,833 - BBR_RS13270 0.28 +0.4 751,833 - BBR_RS13270 0.28 +0.4 751,843 - BBR_RS13270 0.30 +3.5 751,881 + BBR_RS13270 0.34 +0.3 751,901 - BBR_RS13270 0.37 +2.6 751,951 + BBR_RS13270 0.43 +0.4 751,988 + BBR_RS13270 0.47 +0.9 751,988 + BBR_RS13270 0.47 +0.5 751,988 + BBR_RS13270 0.47 -0.2 752,055 - BBR_RS13270 0.56 +0.0 752,055 - BBR_RS13270 0.56 +0.9 752,097 - BBR_RS13270 0.61 -1.1 752,107 + BBR_RS13270 0.62 -0.5 752,138 + BBR_RS13270 0.66 +0.4 752,162 + BBR_RS13270 0.69 +2.3 752,170 - BBR_RS13270 0.70 +0.2 752,190 - BBR_RS13270 0.72 +0.6 752,209 + BBR_RS13270 0.75 -1.9 752,217 - BBR_RS13270 0.76 -0.5 752,217 - BBR_RS13270 0.76 +0.2 752,271 - BBR_RS13270 0.82 +4.2 752,301 + BBR_RS13270 0.86 +1.6 752,307 - BBR_RS13270 0.87 -0.7 752,361 + +0.8 752,366 + +0.6 752,366 + -1.1 752,366 + +0.1 752,366 + -0.6 752,388 + -1.0 752,389 - -0.2 752,408 + -0.2 752,408 + -0.1 752,468 - +2.2 752,492 - +1.3 752,517 - -0.7 752,556 + BBR_RS13275 0.10 -0.4 752,564 - BBR_RS13275 0.11 -0.1 752,605 - BBR_RS13275 0.14 -0.7 752,626 - BBR_RS13275 0.15 -0.1 752,663 + BBR_RS13275 0.18 +0.7 752,669 + BBR_RS13275 0.19 -0.6 752,783 + BBR_RS13275 0.28 -0.1 752,790 - BBR_RS13275 0.28 +0.3 752,811 - BBR_RS13275 0.30 -0.1 752,813 + BBR_RS13275 0.30 -0.6 752,813 - BBR_RS13275 0.30 -0.4 752,821 - BBR_RS13275 0.30 +1.4 752,844 - BBR_RS13275 0.32 +0.8 752,850 - BBR_RS13275 0.33 +0.8 752,852 + BBR_RS13275 0.33 +0.5 752,912 - BBR_RS13275 0.38 -1.8 752,912 - BBR_RS13275 0.38 +0.8 752,984 + BBR_RS13275 0.43 +1.1 752,999 + BBR_RS13275 0.44 -1.7 753,022 + BBR_RS13275 0.46 +0.6 753,037 + BBR_RS13275 0.47 +0.4 753,045 - BBR_RS13275 0.48 -0.7 753,082 + BBR_RS13275 0.51 -0.0 753,082 + BBR_RS13275 0.51 -1.3 753,097 + BBR_RS13275 0.52 -2.2 753,105 + BBR_RS13275 0.52 +0.6 753,109 + BBR_RS13275 0.53 +1.4 753,157 - BBR_RS13275 0.56 -0.7 753,194 + BBR_RS13275 0.59 +0.1 753,200 - BBR_RS13275 0.60 -2.1 753,204 + BBR_RS13275 0.60 +0.7 753,204 + BBR_RS13275 0.60 -1.6 753,212 - BBR_RS13275 0.61 +1.3 753,216 + BBR_RS13275 0.61 -2.5 753,226 + BBR_RS13275 0.62 +0.4 753,249 - BBR_RS13275 0.64 -0.9 753,292 - BBR_RS13275 0.67 +0.8 753,294 - BBR_RS13275 0.67 -0.5 753,350 - BBR_RS13275 0.71 +0.0 753,375 + BBR_RS13275 0.73 -0.2
Or see this region's nucleotide sequence