Experiment: Recovery broth with Bicyclomycin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13245 and BBR_RS13250 overlap by 20 nucleotides BBR_RS13250 and BBR_RS13255 are separated by 234 nucleotides BBR_RS13255 and BBR_RS13260 are separated by 102 nucleotides
BBR_RS13245: BBR_RS13245 - LacI family transcriptional regulator, at 745,242 to 746,486
_RS13245
BBR_RS13250: BBR_RS13250 - alpha-glucosidase, at 746,467 to 748,281
_RS13250
BBR_RS13255: BBR_RS13255 - hypothetical protein, at 748,516 to 748,692
_RS13255
BBR_RS13260: BBR_RS13260 - ABC transporter substrate-binding protein, at 748,795 to 750,414
_RS13260
Position (kb) 746
747
748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 745.488 kb on - strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.493 kb on + strand, within BBR_RS13245 at 745.501 kb on - strand, within BBR_RS13245 at 745.525 kb on + strand, within BBR_RS13245 at 745.527 kb on + strand, within BBR_RS13245 at 745.562 kb on - strand, within BBR_RS13245 at 745.584 kb on + strand, within BBR_RS13245 at 745.592 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.602 kb on - strand, within BBR_RS13245 at 745.607 kb on - strand, within BBR_RS13245 at 745.620 kb on + strand, within BBR_RS13245 at 745.628 kb on - strand, within BBR_RS13245 at 745.674 kb on + strand, within BBR_RS13245 at 745.725 kb on - strand, within BBR_RS13245 at 745.759 kb on + strand, within BBR_RS13245 at 745.769 kb on + strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.829 kb on + strand, within BBR_RS13245 at 745.854 kb on + strand, within BBR_RS13245 at 745.862 kb on - strand, within BBR_RS13245 at 745.879 kb on + strand, within BBR_RS13245 at 745.893 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.953 kb on + strand, within BBR_RS13245 at 745.960 kb on - strand, within BBR_RS13245 at 745.972 kb on - strand, within BBR_RS13245 at 745.976 kb on + strand, within BBR_RS13245 at 745.994 kb on + strand, within BBR_RS13245 at 746.021 kb on - strand, within BBR_RS13245 at 746.049 kb on + strand, within BBR_RS13245 at 746.077 kb on + strand, within BBR_RS13245 at 746.128 kb on + strand, within BBR_RS13245 at 746.133 kb on + strand, within BBR_RS13245 at 746.148 kb on + strand, within BBR_RS13245 at 746.157 kb on - strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.223 kb on + strand, within BBR_RS13245 at 746.238 kb on - strand, within BBR_RS13245 at 746.259 kb on + strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.308 kb on + strand, within BBR_RS13245 at 746.364 kb on + strand at 746.398 kb on + strand at 746.458 kb on - strand at 746.466 kb on + strand at 746.466 kb on + strand at 746.481 kb on - strand at 746.488 kb on + strand at 746.488 kb on + strand at 746.496 kb on - strand at 746.530 kb on - strand at 746.553 kb on + strand at 746.564 kb on + strand at 746.575 kb on + strand at 746.608 kb on - strand at 746.656 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.669 kb on - strand, within BBR_RS13250 at 746.674 kb on - strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.712 kb on - strand, within BBR_RS13250 at 746.758 kb on - strand, within BBR_RS13250 at 746.770 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.832 kb on - strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.873 kb on - strand, within BBR_RS13250 at 746.887 kb on + strand, within BBR_RS13250 at 746.895 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.939 kb on + strand, within BBR_RS13250 at 746.959 kb on + strand, within BBR_RS13250 at 746.985 kb on + strand, within BBR_RS13250 at 746.993 kb on + strand, within BBR_RS13250 at 746.993 kb on - strand, within BBR_RS13250 at 747.015 kb on - strand, within BBR_RS13250 at 747.022 kb on - strand, within BBR_RS13250 at 747.043 kb on + strand, within BBR_RS13250 at 747.051 kb on - strand, within BBR_RS13250 at 747.072 kb on - strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.087 kb on - strand, within BBR_RS13250 at 747.131 kb on - strand, within BBR_RS13250 at 747.142 kb on + strand, within BBR_RS13250 at 747.145 kb on - strand, within BBR_RS13250 at 747.184 kb on + strand, within BBR_RS13250 at 747.194 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.231 kb on - strand, within BBR_RS13250 at 747.233 kb on + strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.237 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.257 kb on - strand, within BBR_RS13250 at 747.263 kb on + strand, within BBR_RS13250 at 747.322 kb on - strand, within BBR_RS13250 at 747.408 kb on + strand, within BBR_RS13250 at 747.418 kb on + strand, within BBR_RS13250 at 747.432 kb on - strand, within BBR_RS13250 at 747.518 kb on + strand, within BBR_RS13250 at 747.531 kb on + strand, within BBR_RS13250 at 747.536 kb on + strand, within BBR_RS13250 at 747.541 kb on + strand, within BBR_RS13250 at 747.544 kb on - strand, within BBR_RS13250 at 747.606 kb on + strand, within BBR_RS13250 at 747.623 kb on + strand, within BBR_RS13250 at 747.631 kb on - strand, within BBR_RS13250 at 747.649 kb on + strand, within BBR_RS13250 at 747.657 kb on + strand, within BBR_RS13250 at 747.665 kb on - strand, within BBR_RS13250 at 747.737 kb on - strand, within BBR_RS13250 at 747.752 kb on + strand, within BBR_RS13250 at 747.825 kb on + strand, within BBR_RS13250 at 747.828 kb on - strand, within BBR_RS13250 at 747.845 kb on + strand, within BBR_RS13250 at 747.877 kb on + strand, within BBR_RS13250 at 747.880 kb on - strand, within BBR_RS13250 at 747.885 kb on - strand, within BBR_RS13250 at 747.896 kb on - strand, within BBR_RS13250 at 747.947 kb on - strand, within BBR_RS13250 at 747.966 kb on + strand, within BBR_RS13250 at 747.974 kb on + strand, within BBR_RS13250 at 747.974 kb on - strand, within BBR_RS13250 at 747.982 kb on - strand, within BBR_RS13250 at 747.996 kb on + strand, within BBR_RS13250 at 748.008 kb on + strand, within BBR_RS13250 at 748.016 kb on - strand, within BBR_RS13250 at 748.022 kb on - strand, within BBR_RS13250 at 748.058 kb on + strand, within BBR_RS13250 at 748.083 kb on + strand, within BBR_RS13250 at 748.086 kb on - strand, within BBR_RS13250 at 748.086 kb on - strand, within BBR_RS13250 at 748.091 kb on - strand, within BBR_RS13250 at 748.148 kb on - strand at 748.157 kb on - strand at 748.213 kb on - strand at 748.218 kb on + strand at 748.236 kb on + strand at 748.239 kb on - strand at 748.243 kb on - strand at 748.244 kb on + strand at 748.244 kb on - strand at 748.308 kb on + strand at 748.308 kb on + strand at 748.414 kb on + strand at 748.415 kb on + strand at 748.428 kb on - strand at 748.428 kb on - strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.491 kb on + strand at 748.499 kb on - strand at 748.499 kb on - strand at 748.552 kb on - strand, within BBR_RS13255 at 748.573 kb on + strand, within BBR_RS13255 at 748.588 kb on - strand, within BBR_RS13255 at 748.618 kb on + strand, within BBR_RS13255 at 748.618 kb on + strand, within BBR_RS13255 at 748.618 kb on - strand, within BBR_RS13255 at 748.642 kb on - strand, within BBR_RS13255 at 748.654 kb on + strand, within BBR_RS13255 at 748.657 kb on - strand, within BBR_RS13255 at 748.662 kb on - strand, within BBR_RS13255 at 748.671 kb on - strand, within BBR_RS13255 at 748.834 kb on + strand at 748.842 kb on + strand at 748.897 kb on + strand at 748.946 kb on - strand at 748.953 kb on + strand at 748.981 kb on + strand, within BBR_RS13260 at 748.996 kb on + strand, within BBR_RS13260 at 749.013 kb on - strand, within BBR_RS13260 at 749.023 kb on - strand, within BBR_RS13260 at 749.023 kb on - strand, within BBR_RS13260 at 749.058 kb on - strand, within BBR_RS13260 at 749.078 kb on + strand, within BBR_RS13260 at 749.097 kb on + strand, within BBR_RS13260 at 749.115 kb on + strand, within BBR_RS13260 at 749.146 kb on - strand, within BBR_RS13260 at 749.146 kb on - strand, within BBR_RS13260 at 749.158 kb on + strand, within BBR_RS13260 at 749.158 kb on + strand, within BBR_RS13260 at 749.165 kb on - strand, within BBR_RS13260 at 749.181 kb on + strand, within BBR_RS13260 at 749.201 kb on - strand, within BBR_RS13260 at 749.244 kb on - strand, within BBR_RS13260 at 749.244 kb on - strand, within BBR_RS13260 at 749.276 kb on - strand, within BBR_RS13260 at 749.276 kb on - strand, within BBR_RS13260
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Bicyclomycin 0.07 mM remove 745,488 - BBR_RS13245 0.20 -0.6 745,492 + BBR_RS13245 0.20 +0.2 745,492 + BBR_RS13245 0.20 +1.8 745,492 + BBR_RS13245 0.20 +1.0 745,493 + BBR_RS13245 0.20 +0.2 745,501 - BBR_RS13245 0.21 +1.1 745,525 + BBR_RS13245 0.23 +0.1 745,527 + BBR_RS13245 0.23 +0.8 745,562 - BBR_RS13245 0.26 -0.6 745,584 + BBR_RS13245 0.27 +0.5 745,592 - BBR_RS13245 0.28 -0.3 745,594 - BBR_RS13245 0.28 +2.7 745,594 - BBR_RS13245 0.28 -0.2 745,602 - BBR_RS13245 0.29 -0.5 745,607 - BBR_RS13245 0.29 +0.9 745,620 + BBR_RS13245 0.30 -1.1 745,628 - BBR_RS13245 0.31 +1.9 745,674 + BBR_RS13245 0.35 -1.4 745,725 - BBR_RS13245 0.39 +1.0 745,759 + BBR_RS13245 0.42 +1.3 745,769 + BBR_RS13245 0.42 +0.4 745,777 - BBR_RS13245 0.43 +1.2 745,777 - BBR_RS13245 0.43 -0.2 745,779 + BBR_RS13245 0.43 -0.3 745,779 + BBR_RS13245 0.43 -0.5 745,829 + BBR_RS13245 0.47 -0.1 745,854 + BBR_RS13245 0.49 +0.1 745,862 - BBR_RS13245 0.50 +0.5 745,879 + BBR_RS13245 0.51 -1.9 745,893 + BBR_RS13245 0.52 -0.5 745,952 + BBR_RS13245 0.57 -1.2 745,952 + BBR_RS13245 0.57 -1.0 745,953 + BBR_RS13245 0.57 -0.7 745,960 - BBR_RS13245 0.58 -0.5 745,972 - BBR_RS13245 0.59 +1.2 745,976 + BBR_RS13245 0.59 -0.7 745,994 + BBR_RS13245 0.60 +0.8 746,021 - BBR_RS13245 0.63 +0.5 746,049 + BBR_RS13245 0.65 -0.4 746,077 + BBR_RS13245 0.67 -0.4 746,128 + BBR_RS13245 0.71 -0.5 746,133 + BBR_RS13245 0.72 -0.7 746,148 + BBR_RS13245 0.73 +0.8 746,157 - BBR_RS13245 0.73 -0.4 746,210 + BBR_RS13245 0.78 -0.3 746,210 + BBR_RS13245 0.78 +0.0 746,223 + BBR_RS13245 0.79 -0.8 746,238 - BBR_RS13245 0.80 -0.1 746,259 + BBR_RS13245 0.82 -1.2 746,290 - BBR_RS13245 0.84 -0.7 746,290 - BBR_RS13245 0.84 -1.3 746,290 - BBR_RS13245 0.84 -0.9 746,308 + BBR_RS13245 0.86 +0.1 746,364 + +0.1 746,398 + -2.9 746,458 - -0.7 746,466 + -0.9 746,466 + -1.5 746,481 - -0.1 746,488 + +1.3 746,488 + +0.7 746,496 - -0.8 746,530 - +0.5 746,553 + +0.5 746,564 + -0.1 746,575 + -1.9 746,608 - -0.2 746,656 + BBR_RS13250 0.10 -0.7 746,661 + BBR_RS13250 0.11 +1.0 746,661 + BBR_RS13250 0.11 +2.2 746,669 - BBR_RS13250 0.11 -0.4 746,674 - BBR_RS13250 0.11 -1.8 746,677 + BBR_RS13250 0.12 +0.5 746,677 + BBR_RS13250 0.12 +0.8 746,677 + BBR_RS13250 0.12 -0.6 746,679 + BBR_RS13250 0.12 -1.3 746,679 + BBR_RS13250 0.12 +1.6 746,685 - BBR_RS13250 0.12 +0.4 746,685 - BBR_RS13250 0.12 -0.1 746,685 - BBR_RS13250 0.12 -0.7 746,712 - BBR_RS13250 0.13 +0.0 746,758 - BBR_RS13250 0.16 +0.3 746,770 - BBR_RS13250 0.17 -0.9 746,792 - BBR_RS13250 0.18 -0.6 746,792 - BBR_RS13250 0.18 -0.9 746,832 - BBR_RS13250 0.20 -0.4 746,865 + BBR_RS13250 0.22 -0.8 746,865 + BBR_RS13250 0.22 -0.3 746,873 - BBR_RS13250 0.22 -0.8 746,887 + BBR_RS13250 0.23 +0.5 746,895 - BBR_RS13250 0.24 +1.1 746,899 - BBR_RS13250 0.24 +1.4 746,899 - BBR_RS13250 0.24 -1.2 746,939 + BBR_RS13250 0.26 +0.4 746,959 + BBR_RS13250 0.27 -1.4 746,985 + BBR_RS13250 0.29 -1.2 746,993 + BBR_RS13250 0.29 -0.2 746,993 - BBR_RS13250 0.29 -0.4 747,015 - BBR_RS13250 0.30 -0.1 747,022 - BBR_RS13250 0.31 -0.5 747,043 + BBR_RS13250 0.32 -0.6 747,051 - BBR_RS13250 0.32 +2.9 747,072 - BBR_RS13250 0.33 +0.0 747,079 + BBR_RS13250 0.34 +1.0 747,079 + BBR_RS13250 0.34 +1.3 747,079 + BBR_RS13250 0.34 -1.1 747,079 + BBR_RS13250 0.34 -0.4 747,079 + BBR_RS13250 0.34 +0.3 747,079 + BBR_RS13250 0.34 +0.2 747,079 + BBR_RS13250 0.34 +1.8 747,087 - BBR_RS13250 0.34 -0.2 747,131 - BBR_RS13250 0.37 +0.6 747,142 + BBR_RS13250 0.37 -1.0 747,145 - BBR_RS13250 0.37 +0.2 747,184 + BBR_RS13250 0.40 +1.3 747,194 + BBR_RS13250 0.40 +0.6 747,228 + BBR_RS13250 0.42 -0.6 747,228 + BBR_RS13250 0.42 -1.4 747,228 + BBR_RS13250 0.42 -1.6 747,231 - BBR_RS13250 0.42 -0.8 747,233 + BBR_RS13250 0.42 -0.7 747,236 - BBR_RS13250 0.42 +0.8 747,236 - BBR_RS13250 0.42 +0.5 747,236 - BBR_RS13250 0.42 +2.7 747,237 - BBR_RS13250 0.42 -0.4 747,252 - BBR_RS13250 0.43 -1.0 747,252 - BBR_RS13250 0.43 -1.8 747,257 - BBR_RS13250 0.44 -0.8 747,263 + BBR_RS13250 0.44 +0.1 747,322 - BBR_RS13250 0.47 +0.1 747,408 + BBR_RS13250 0.52 +0.9 747,418 + BBR_RS13250 0.52 +0.2 747,432 - BBR_RS13250 0.53 +1.2 747,518 + BBR_RS13250 0.58 +0.7 747,531 + BBR_RS13250 0.59 +1.7 747,536 + BBR_RS13250 0.59 +0.1 747,541 + BBR_RS13250 0.59 -0.1 747,544 - BBR_RS13250 0.59 +2.2 747,606 + BBR_RS13250 0.63 +0.6 747,623 + BBR_RS13250 0.64 +0.5 747,631 - BBR_RS13250 0.64 -0.2 747,649 + BBR_RS13250 0.65 -0.9 747,657 + BBR_RS13250 0.66 -2.0 747,665 - BBR_RS13250 0.66 -1.3 747,737 - BBR_RS13250 0.70 +0.5 747,752 + BBR_RS13250 0.71 -0.1 747,825 + BBR_RS13250 0.75 -0.4 747,828 - BBR_RS13250 0.75 +0.6 747,845 + BBR_RS13250 0.76 -0.5 747,877 + BBR_RS13250 0.78 -0.7 747,880 - BBR_RS13250 0.78 +0.7 747,885 - BBR_RS13250 0.78 -0.4 747,896 - BBR_RS13250 0.79 +0.2 747,947 - BBR_RS13250 0.82 +1.0 747,966 + BBR_RS13250 0.83 +1.8 747,974 + BBR_RS13250 0.83 -1.8 747,974 - BBR_RS13250 0.83 -0.8 747,982 - BBR_RS13250 0.83 -0.7 747,996 + BBR_RS13250 0.84 -1.8 748,008 + BBR_RS13250 0.85 -1.0 748,016 - BBR_RS13250 0.85 +0.5 748,022 - BBR_RS13250 0.86 +2.5 748,058 + BBR_RS13250 0.88 +1.1 748,083 + BBR_RS13250 0.89 -0.3 748,086 - BBR_RS13250 0.89 -1.1 748,086 - BBR_RS13250 0.89 +0.3 748,091 - BBR_RS13250 0.89 -1.5 748,148 - +0.1 748,157 - -0.4 748,213 - -0.3 748,218 + +0.5 748,236 + +0.8 748,239 - +0.1 748,243 - -0.6 748,244 + -1.0 748,244 - -1.0 748,308 + -0.0 748,308 + -2.1 748,414 + +1.1 748,415 + +2.1 748,428 - -1.0 748,428 - -1.2 748,471 + +1.2 748,471 + -0.8 748,471 + -0.7 748,471 + +1.2 748,479 - -0.5 748,479 - +0.1 748,479 - +0.3 748,479 - -0.5 748,479 - -0.5 748,491 + +0.5 748,499 - +0.7 748,499 - +0.4 748,552 - BBR_RS13255 0.20 -0.3 748,573 + BBR_RS13255 0.32 +1.0 748,588 - BBR_RS13255 0.41 -0.6 748,618 + BBR_RS13255 0.58 +0.7 748,618 + BBR_RS13255 0.58 -0.9 748,618 - BBR_RS13255 0.58 +1.0 748,642 - BBR_RS13255 0.71 +0.2 748,654 + BBR_RS13255 0.78 -0.7 748,657 - BBR_RS13255 0.80 -0.7 748,662 - BBR_RS13255 0.82 +0.1 748,671 - BBR_RS13255 0.88 +0.0 748,834 + -0.2 748,842 + +1.5 748,897 + -1.3 748,946 - -1.6 748,953 + +0.6 748,981 + BBR_RS13260 0.11 +0.2 748,996 + BBR_RS13260 0.12 +0.5 749,013 - BBR_RS13260 0.13 -0.2 749,023 - BBR_RS13260 0.14 +0.4 749,023 - BBR_RS13260 0.14 +1.0 749,058 - BBR_RS13260 0.16 +0.8 749,078 + BBR_RS13260 0.17 -0.5 749,097 + BBR_RS13260 0.19 +1.0 749,115 + BBR_RS13260 0.20 +0.1 749,146 - BBR_RS13260 0.22 +1.2 749,146 - BBR_RS13260 0.22 +1.5 749,158 + BBR_RS13260 0.22 -1.0 749,158 + BBR_RS13260 0.22 -1.8 749,165 - BBR_RS13260 0.23 -0.5 749,181 + BBR_RS13260 0.24 -1.3 749,201 - BBR_RS13260 0.25 +0.8 749,244 - BBR_RS13260 0.28 +0.2 749,244 - BBR_RS13260 0.28 +2.1 749,276 - BBR_RS13260 0.30 +0.4 749,276 - BBR_RS13260 0.30 -0.5
Or see this region's nucleotide sequence