Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4652 and BT4653 are separated by 4 nucleotides BT4653 and BT4654 are separated by 55 nucleotides
BT4652: BT4652 - heparan sulfate cleaving enzyme (from data) , at 6,085,860 to 6,088,478
BT4652
BT4653: BT4653 - glucose/galactose transporter (NCBI ptt file), at 6,088,483 to 6,089,730
BT4653
BT4654: BT4654 - ROK family transcriptional repressor, with glucokinase domain (NCBI ptt file), at 6,089,786 to 6,090,739
BT4654
Position (kb)
6088
6089
6090 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6087.509 kb on - strand, within BT4652 at 6087.509 kb on - strand, within BT4652 at 6087.586 kb on + strand, within BT4652 at 6087.587 kb on - strand, within BT4652 at 6087.587 kb on - strand, within BT4652 at 6087.599 kb on - strand, within BT4652 at 6087.609 kb on - strand, within BT4652 at 6087.653 kb on - strand, within BT4652 at 6087.656 kb on + strand, within BT4652 at 6087.706 kb on + strand, within BT4652 at 6087.741 kb on + strand, within BT4652 at 6087.754 kb on - strand, within BT4652 at 6087.817 kb on - strand, within BT4652 at 6087.817 kb on - strand, within BT4652 at 6087.910 kb on + strand, within BT4652 at 6088.040 kb on + strand, within BT4652 at 6088.061 kb on - strand, within BT4652 at 6088.109 kb on - strand, within BT4652 at 6088.231 kb on + strand at 6088.232 kb on - strand at 6088.443 kb on + strand at 6088.560 kb on + strand at 6088.560 kb on + strand at 6088.560 kb on + strand at 6088.590 kb on + strand at 6088.590 kb on + strand at 6088.591 kb on - strand at 6088.624 kb on - strand, within BT4653 at 6088.810 kb on + strand, within BT4653 at 6088.831 kb on + strand, within BT4653 at 6088.847 kb on - strand, within BT4653 at 6088.968 kb on - strand, within BT4653 at 6088.995 kb on + strand, within BT4653 at 6089.022 kb on - strand, within BT4653 at 6089.051 kb on - strand, within BT4653 at 6089.054 kb on + strand, within BT4653 at 6089.054 kb on + strand, within BT4653 at 6089.055 kb on - strand, within BT4653 at 6089.055 kb on - strand, within BT4653 at 6089.170 kb on + strand, within BT4653 at 6089.259 kb on + strand, within BT4653 at 6089.259 kb on + strand, within BT4653 at 6089.292 kb on - strand, within BT4653 at 6089.295 kb on + strand, within BT4653 at 6089.334 kb on + strand, within BT4653 at 6089.453 kb on - strand, within BT4653 at 6089.469 kb on + strand, within BT4653 at 6089.470 kb on - strand, within BT4653 at 6089.475 kb on + strand, within BT4653 at 6089.476 kb on - strand, within BT4653 at 6089.578 kb on - strand, within BT4653 at 6089.787 kb on + strand at 6089.788 kb on - strand at 6089.987 kb on - strand, within BT4654 at 6090.069 kb on + strand, within BT4654 at 6090.096 kb on - strand, within BT4654 at 6090.192 kb on - strand, within BT4654 at 6090.220 kb on + strand, within BT4654 at 6090.224 kb on + strand, within BT4654 at 6090.225 kb on - strand, within BT4654 at 6090.317 kb on + strand, within BT4654 at 6090.318 kb on - strand, within BT4654 at 6090.360 kb on + strand, within BT4654 at 6090.503 kb on - strand, within BT4654 at 6090.548 kb on - strand, within BT4654 at 6090.548 kb on - strand, within BT4654 at 6090.596 kb on + strand, within BT4654 at 6090.720 kb on - strand at 6090.722 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 6,087,509 - BT4652 0.63 -3.5 6,087,509 - BT4652 0.63 -4.4 6,087,586 + BT4652 0.66 -1.3 6,087,587 - BT4652 0.66 -4.5 6,087,587 - BT4652 0.66 -3.1 6,087,599 - BT4652 0.66 -2.7 6,087,609 - BT4652 0.67 -2.1 6,087,653 - BT4652 0.68 -0.3 6,087,656 + BT4652 0.69 -2.1 6,087,706 + BT4652 0.70 -3.2 6,087,741 + BT4652 0.72 -4.1 6,087,754 - BT4652 0.72 -2.7 6,087,817 - BT4652 0.75 -2.2 6,087,817 - BT4652 0.75 -1.7 6,087,910 + BT4652 0.78 -2.0 6,088,040 + BT4652 0.83 -1.7 6,088,061 - BT4652 0.84 -1.3 6,088,109 - BT4652 0.86 -0.9 6,088,231 + -2.8 6,088,232 - -2.1 6,088,443 + -2.5 6,088,560 + -0.9 6,088,560 + -2.8 6,088,560 + -2.8 6,088,590 + -4.0 6,088,590 + -1.5 6,088,591 - -3.2 6,088,624 - BT4653 0.11 -1.9 6,088,810 + BT4653 0.26 -1.9 6,088,831 + BT4653 0.28 -2.8 6,088,847 - BT4653 0.29 -1.9 6,088,968 - BT4653 0.39 +0.0 6,088,995 + BT4653 0.41 -0.3 6,089,022 - BT4653 0.43 -1.9 6,089,051 - BT4653 0.46 -3.3 6,089,054 + BT4653 0.46 -0.7 6,089,054 + BT4653 0.46 -3.0 6,089,055 - BT4653 0.46 -0.3 6,089,055 - BT4653 0.46 +1.7 6,089,170 + BT4653 0.55 -4.5 6,089,259 + BT4653 0.62 -1.3 6,089,259 + BT4653 0.62 -2.5 6,089,292 - BT4653 0.65 -1.5 6,089,295 + BT4653 0.65 +0.7 6,089,334 + BT4653 0.68 -2.8 6,089,453 - BT4653 0.78 -0.3 6,089,469 + BT4653 0.79 -0.3 6,089,470 - BT4653 0.79 -2.0 6,089,475 + BT4653 0.79 -1.7 6,089,476 - BT4653 0.80 -1.5 6,089,578 - BT4653 0.88 -4.2 6,089,787 + -3.0 6,089,788 - -2.7 6,089,987 - BT4654 0.21 -2.2 6,090,069 + BT4654 0.30 -2.7 6,090,096 - BT4654 0.32 -1.3 6,090,192 - BT4654 0.43 -2.3 6,090,220 + BT4654 0.45 -2.5 6,090,224 + BT4654 0.46 +0.7 6,090,225 - BT4654 0.46 -2.3 6,090,317 + BT4654 0.56 -2.7 6,090,318 - BT4654 0.56 -0.9 6,090,360 + BT4654 0.60 -1.9 6,090,503 - BT4654 0.75 -2.5 6,090,548 - BT4654 0.80 +1.3 6,090,548 - BT4654 0.80 -1.7 6,090,596 + BT4654 0.85 -2.1 6,090,720 - -2.7 6,090,722 - -0.6
Or see this region's nucleotide sequence