Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2065 and BT2066 are separated by 196 nucleotides BT2066 and BT2067 overlap by 4 nucleotides
BT2065: BT2065 - putative TonB-linked outer membrane receptor (NCBI ptt file), at 2,584,894 to 2,587,284
BT2065
BT2066: BT2066 - putative oxidoreductase (NCBI ptt file), at 2,587,481 to 2,588,332
BT2066
BT2067: BT2067 - putative NADH:flavin oxidoreductase (NCBI ptt file), at 2,588,329 to 2,589,552
BT2067
Position (kb)
2587
2588
2589 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2586.518 kb on + strand, within BT2065 at 2586.525 kb on + strand, within BT2065 at 2586.526 kb on - strand, within BT2065 at 2586.531 kb on + strand, within BT2065 at 2586.592 kb on + strand, within BT2065 at 2586.593 kb on - strand, within BT2065 at 2586.653 kb on + strand, within BT2065 at 2586.712 kb on + strand, within BT2065 at 2586.861 kb on + strand, within BT2065 at 2586.861 kb on + strand, within BT2065 at 2586.867 kb on + strand, within BT2065 at 2586.944 kb on - strand, within BT2065 at 2586.982 kb on + strand, within BT2065 at 2586.995 kb on - strand, within BT2065 at 2587.067 kb on + strand at 2587.067 kb on + strand at 2587.180 kb on - strand at 2587.276 kb on - strand at 2587.292 kb on + strand at 2587.297 kb on + strand at 2587.308 kb on + strand at 2587.309 kb on - strand at 2587.309 kb on - strand at 2587.315 kb on + strand at 2587.317 kb on + strand at 2587.318 kb on - strand at 2587.319 kb on + strand at 2587.320 kb on - strand at 2587.346 kb on + strand at 2587.432 kb on - strand at 2587.432 kb on - strand at 2587.462 kb on - strand at 2587.717 kb on + strand, within BT2066 at 2587.805 kb on - strand, within BT2066 at 2587.911 kb on + strand, within BT2066 at 2587.912 kb on - strand, within BT2066 at 2587.912 kb on - strand at 2587.912 kb on - strand, within BT2066 at 2587.931 kb on + strand, within BT2066 at 2587.996 kb on - strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.240 kb on - strand, within BT2066 at 2588.242 kb on + strand, within BT2066 at 2588.417 kb on + strand at 2588.417 kb on + strand at 2588.435 kb on + strand at 2588.455 kb on - strand, within BT2067 at 2588.552 kb on - strand, within BT2067 at 2588.649 kb on - strand, within BT2067 at 2588.706 kb on - strand, within BT2067 at 2588.772 kb on - strand, within BT2067 at 2589.020 kb on + strand, within BT2067 at 2589.021 kb on - strand, within BT2067 at 2589.048 kb on - strand, within BT2067 at 2589.064 kb on + strand, within BT2067 at 2589.064 kb on + strand, within BT2067 at 2589.192 kb on + strand, within BT2067 at 2589.237 kb on + strand, within BT2067 at 2589.238 kb on - strand, within BT2067 at 2589.238 kb on - strand, within BT2067
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 2,586,518 + BT2065 0.68 -2.7 2,586,525 + BT2065 0.68 -1.1 2,586,526 - BT2065 0.68 -3.0 2,586,531 + BT2065 0.68 -1.8 2,586,592 + BT2065 0.71 -0.3 2,586,593 - BT2065 0.71 +0.7 2,586,653 + BT2065 0.74 -0.8 2,586,712 + BT2065 0.76 -2.1 2,586,861 + BT2065 0.82 -0.6 2,586,861 + BT2065 0.82 -2.6 2,586,867 + BT2065 0.83 -2.6 2,586,944 - BT2065 0.86 -1.6 2,586,982 + BT2065 0.87 -0.6 2,586,995 - BT2065 0.88 -2.1 2,587,067 + -1.1 2,587,067 + -2.3 2,587,180 - -1.8 2,587,276 - -2.6 2,587,292 + -1.4 2,587,297 + -1.1 2,587,308 + -0.8 2,587,309 - +0.2 2,587,309 - +1.9 2,587,315 + +0.2 2,587,317 + +0.4 2,587,318 - +2.7 2,587,319 + -0.1 2,587,320 - -0.8 2,587,346 + +0.6 2,587,432 - +1.5 2,587,432 - -1.6 2,587,462 - -0.3 2,587,717 + BT2066 0.28 -0.7 2,587,805 - BT2066 0.38 +1.2 2,587,911 + BT2066 0.50 +1.9 2,587,912 - BT2066 0.51 -1.2 2,587,912 - +0.7 2,587,912 - BT2066 0.51 -0.8 2,587,931 + BT2066 0.53 -0.4 2,587,996 - BT2066 0.60 -2.3 2,588,239 + BT2066 0.89 +1.3 2,588,239 + BT2066 0.89 +0.4 2,588,239 + BT2066 0.89 +2.2 2,588,240 - BT2066 0.89 +1.4 2,588,242 + BT2066 0.89 +0.6 2,588,417 + -2.3 2,588,417 + -2.6 2,588,435 + +1.0 2,588,455 - BT2067 0.10 +2.1 2,588,552 - BT2067 0.18 -2.6 2,588,649 - BT2067 0.26 -0.3 2,588,706 - BT2067 0.31 +0.9 2,588,772 - BT2067 0.36 -1.3 2,589,020 + BT2067 0.56 +0.7 2,589,021 - BT2067 0.57 -1.5 2,589,048 - BT2067 0.59 -1.4 2,589,064 + BT2067 0.60 +1.7 2,589,064 + BT2067 0.60 -2.8 2,589,192 + BT2067 0.71 +1.0 2,589,237 + BT2067 0.74 +0.2 2,589,238 - BT2067 0.74 -2.0 2,589,238 - BT2067 0.74 +0.7
Or see this region's nucleotide sequence