Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1885 and BT1886 are separated by 94 nucleotides BT1886 and BT1887 are separated by 339 nucleotides BT1887 and BT1888 are separated by 319 nucleotides
BT1885: BT1885 - putative ATP-dependent RNA helicase (NCBI ptt file), at 2,372,322 to 2,373,446
BT1885
BT1886: BT1886 - conserved hypothetical protein (NCBI ptt file), at 2,373,541 to 2,374,638
BT1886
BT1887: BT1887 - putative RNA-binding protein rbpA (NCBI ptt file), at 2,374,978 to 2,375,280
BT1887
BT1888: BT1888 - transcriptional regulator, LuxR family (NCBI ptt file), at 2,375,600 to 2,376,376
BT1888
Position (kb)
2373
2374
2375 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2372.627 kb on + strand, within BT1885 at 2372.681 kb on + strand, within BT1885 at 2372.702 kb on + strand, within BT1885 at 2372.707 kb on - strand, within BT1885 at 2372.792 kb on + strand, within BT1885 at 2372.792 kb on + strand, within BT1885 at 2372.800 kb on - strand, within BT1885 at 2372.819 kb on + strand, within BT1885 at 2372.820 kb on - strand, within BT1885 at 2372.939 kb on + strand, within BT1885 at 2372.952 kb on - strand, within BT1885 at 2372.952 kb on - strand, within BT1885 at 2373.015 kb on + strand, within BT1885 at 2373.017 kb on + strand, within BT1885 at 2373.146 kb on + strand, within BT1885 at 2373.149 kb on - strand, within BT1885 at 2373.267 kb on + strand, within BT1885 at 2373.273 kb on - strand, within BT1885 at 2373.321 kb on + strand, within BT1885 at 2373.346 kb on - strand at 2373.378 kb on + strand at 2373.425 kb on + strand at 2373.430 kb on - strand at 2373.447 kb on - strand at 2373.489 kb on + strand at 2373.489 kb on + strand at 2373.490 kb on - strand at 2373.494 kb on + strand at 2373.681 kb on + strand, within BT1886 at 2373.789 kb on + strand, within BT1886 at 2373.840 kb on + strand, within BT1886 at 2373.867 kb on + strand, within BT1886 at 2373.869 kb on + strand, within BT1886 at 2373.870 kb on - strand, within BT1886 at 2373.969 kb on + strand, within BT1886 at 2374.076 kb on + strand, within BT1886 at 2374.160 kb on + strand, within BT1886 at 2374.164 kb on + strand, within BT1886 at 2374.165 kb on - strand, within BT1886 at 2374.216 kb on + strand, within BT1886 at 2374.248 kb on + strand, within BT1886 at 2374.256 kb on + strand, within BT1886 at 2374.332 kb on + strand, within BT1886 at 2374.374 kb on + strand, within BT1886 at 2374.442 kb on + strand, within BT1886 at 2374.442 kb on + strand, within BT1886 at 2374.489 kb on + strand at 2374.489 kb on + strand, within BT1886 at 2374.490 kb on - strand, within BT1886 at 2374.568 kb on - strand at 2374.582 kb on - strand at 2374.590 kb on + strand at 2374.621 kb on + strand at 2374.663 kb on + strand at 2374.668 kb on + strand at 2374.668 kb on + strand at 2374.780 kb on + strand at 2374.781 kb on - strand at 2374.812 kb on - strand at 2374.813 kb on + strand at 2374.814 kb on - strand at 2374.815 kb on + strand at 2374.926 kb on + strand at 2374.926 kb on + strand at 2374.968 kb on + strand at 2375.067 kb on + strand, within BT1887 at 2375.082 kb on - strand, within BT1887 at 2375.257 kb on + strand at 2375.347 kb on - strand at 2375.349 kb on + strand at 2375.349 kb on + strand at 2375.357 kb on + strand at 2375.358 kb on - strand at 2375.362 kb on + strand at 2375.431 kb on + strand at 2375.543 kb on - strand at 2375.545 kb on + strand at 2375.545 kb on + strand at 2375.555 kb on + strand at 2375.555 kb on + strand at 2375.572 kb on + strand at 2375.598 kb on - strand at 2375.598 kb on - strand at 2375.616 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 2,372,627 + BT1885 0.27 +0.1 2,372,681 + BT1885 0.32 +1.3 2,372,702 + BT1885 0.34 +1.0 2,372,707 - BT1885 0.34 +0.3 2,372,792 + BT1885 0.42 +0.2 2,372,792 + BT1885 0.42 +1.5 2,372,800 - BT1885 0.42 -1.2 2,372,819 + BT1885 0.44 +0.5 2,372,820 - BT1885 0.44 -0.3 2,372,939 + BT1885 0.55 +1.7 2,372,952 - BT1885 0.56 -0.8 2,372,952 - BT1885 0.56 +2.5 2,373,015 + BT1885 0.62 +1.4 2,373,017 + BT1885 0.62 -1.2 2,373,146 + BT1885 0.73 -1.7 2,373,149 - BT1885 0.74 +0.1 2,373,267 + BT1885 0.84 +1.9 2,373,273 - BT1885 0.85 -2.2 2,373,321 + BT1885 0.89 -1.4 2,373,346 - -2.1 2,373,378 + +2.0 2,373,425 + +1.1 2,373,430 - +0.4 2,373,447 - -0.8 2,373,489 + -1.2 2,373,489 + -3.6 2,373,490 - -2.6 2,373,494 + +0.0 2,373,681 + BT1886 0.13 -2.7 2,373,789 + BT1886 0.23 -2.2 2,373,840 + BT1886 0.27 -2.4 2,373,867 + BT1886 0.30 +0.7 2,373,869 + BT1886 0.30 -2.1 2,373,870 - BT1886 0.30 -0.2 2,373,969 + BT1886 0.39 -1.6 2,374,076 + BT1886 0.49 -1.1 2,374,160 + BT1886 0.56 -1.6 2,374,164 + BT1886 0.57 -0.0 2,374,165 - BT1886 0.57 +0.8 2,374,216 + BT1886 0.61 -1.2 2,374,248 + BT1886 0.64 -0.8 2,374,256 + BT1886 0.65 -1.6 2,374,332 + BT1886 0.72 -0.6 2,374,374 + BT1886 0.76 -1.7 2,374,442 + BT1886 0.82 -2.6 2,374,442 + BT1886 0.82 -0.0 2,374,489 + +0.8 2,374,489 + BT1886 0.86 -2.1 2,374,490 - BT1886 0.86 +0.2 2,374,568 - +0.4 2,374,582 - +0.8 2,374,590 + +2.1 2,374,621 + -2.0 2,374,663 + +0.8 2,374,668 + -0.2 2,374,668 + -4.7 2,374,780 + -1.4 2,374,781 - -1.9 2,374,812 - +1.8 2,374,813 + +0.3 2,374,814 - -0.7 2,374,815 + +0.8 2,374,926 + -1.2 2,374,926 + +0.9 2,374,968 + -1.1 2,375,067 + BT1887 0.29 -0.6 2,375,082 - BT1887 0.34 +0.3 2,375,257 + -1.7 2,375,347 - -1.6 2,375,349 + -1.0 2,375,349 + -0.3 2,375,357 + -2.4 2,375,358 - +0.0 2,375,362 + +0.7 2,375,431 + -0.4 2,375,543 - +1.0 2,375,545 + -2.6 2,375,545 + +0.8 2,375,555 + -1.6 2,375,555 + -1.0 2,375,572 + -1.1 2,375,598 - +3.2 2,375,598 - +1.5 2,375,616 + -1.5
Or see this region's nucleotide sequence