Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1449 and BT1450 are separated by 55 nucleotides BT1450 and BT1451 are separated by 245 nucleotides BT1451 and BT1452 are separated by 24 nucleotides
BT1449: BT1449 - biotin carboxylase (NCBI ptt file), at 1,784,196 to 1,785,707
BT1449
BT1450: BT1450 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 1,785,763 to 1,787,307
BT1450
BT1451: BT1451 - hypothetical protein (NCBI ptt file), at 1,787,553 to 1,788,140
BT1451
BT1452: BT1452 - conserved hypothetical protein (NCBI ptt file), at 1,788,165 to 1,789,619
BT1452
Position (kb)
1785
1786
1787
1788 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1784.802 kb on - strand, within BT1449 at 1784.827 kb on - strand, within BT1449 at 1784.827 kb on - strand, within BT1449 at 1784.905 kb on + strand, within BT1449 at 1785.058 kb on + strand, within BT1449 at 1785.058 kb on + strand, within BT1449 at 1785.106 kb on + strand, within BT1449 at 1785.107 kb on - strand, within BT1449 at 1785.116 kb on + strand, within BT1449 at 1785.117 kb on - strand, within BT1449 at 1785.248 kb on + strand at 1785.248 kb on + strand, within BT1449 at 1785.256 kb on - strand, within BT1449 at 1785.258 kb on + strand, within BT1449 at 1785.537 kb on - strand, within BT1449 at 1785.666 kb on - strand at 1785.669 kb on + strand at 1785.762 kb on - strand at 1785.881 kb on + strand at 1785.991 kb on + strand, within BT1450 at 1785.997 kb on - strand, within BT1450 at 1786.092 kb on - strand, within BT1450 at 1786.104 kb on - strand, within BT1450 at 1786.110 kb on + strand, within BT1450 at 1786.176 kb on + strand, within BT1450 at 1786.249 kb on + strand, within BT1450 at 1786.252 kb on - strand, within BT1450 at 1786.374 kb on + strand, within BT1450 at 1786.447 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.561 kb on - strand, within BT1450 at 1786.561 kb on - strand, within BT1450 at 1786.585 kb on - strand, within BT1450 at 1786.598 kb on + strand, within BT1450 at 1786.599 kb on - strand, within BT1450 at 1786.599 kb on - strand, within BT1450 at 1786.605 kb on + strand, within BT1450 at 1786.944 kb on + strand, within BT1450 at 1787.023 kb on + strand, within BT1450 at 1787.131 kb on - strand, within BT1450 at 1787.152 kb on - strand, within BT1450 at 1787.307 kb on + strand at 1787.308 kb on - strand at 1787.318 kb on + strand at 1787.398 kb on - strand at 1787.430 kb on - strand at 1787.501 kb on + strand at 1787.515 kb on + strand at 1787.565 kb on - strand at 1787.641 kb on - strand, within BT1451 at 1787.755 kb on - strand, within BT1451 at 1787.804 kb on - strand, within BT1451 at 1787.965 kb on - strand, within BT1451 at 1787.975 kb on + strand, within BT1451 at 1788.038 kb on + strand, within BT1451 at 1788.038 kb on + strand, within BT1451 at 1788.126 kb on + strand at 1788.180 kb on - strand at 1788.191 kb on + strand at 1788.192 kb on - strand at 1788.193 kb on + strand at 1788.211 kb on + strand at 1788.212 kb on - strand at 1788.265 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 1,784,802 - BT1449 0.40 +0.8 1,784,827 - BT1449 0.42 +0.4 1,784,827 - BT1449 0.42 -2.1 1,784,905 + BT1449 0.47 +0.2 1,785,058 + BT1449 0.57 -1.5 1,785,058 + BT1449 0.57 +0.5 1,785,106 + BT1449 0.60 -3.0 1,785,107 - BT1449 0.60 +0.5 1,785,116 + BT1449 0.61 +0.8 1,785,117 - BT1449 0.61 -2.1 1,785,248 + -1.1 1,785,248 + BT1449 0.70 -1.8 1,785,256 - BT1449 0.70 -1.8 1,785,258 + BT1449 0.70 +0.6 1,785,537 - BT1449 0.89 -0.8 1,785,666 - -0.5 1,785,669 + -1.1 1,785,762 - -2.8 1,785,881 + -1.7 1,785,991 + BT1450 0.15 -1.5 1,785,997 - BT1450 0.15 -3.4 1,786,092 - BT1450 0.21 -2.3 1,786,104 - BT1450 0.22 -2.8 1,786,110 + BT1450 0.22 +2.1 1,786,176 + BT1450 0.27 -2.1 1,786,249 + BT1450 0.31 -0.5 1,786,252 - BT1450 0.32 -2.6 1,786,374 + BT1450 0.40 -1.9 1,786,447 + BT1450 0.44 +1.2 1,786,461 + BT1450 0.45 -1.5 1,786,461 + BT1450 0.45 -1.1 1,786,461 + BT1450 0.45 -1.4 1,786,561 - BT1450 0.52 -2.5 1,786,561 - BT1450 0.52 -1.1 1,786,585 - BT1450 0.53 -0.6 1,786,598 + BT1450 0.54 -2.5 1,786,599 - BT1450 0.54 +0.4 1,786,599 - BT1450 0.54 -0.3 1,786,605 + BT1450 0.54 +0.5 1,786,944 + BT1450 0.76 +0.1 1,787,023 + BT1450 0.82 -1.7 1,787,131 - BT1450 0.89 -2.3 1,787,152 - BT1450 0.90 +0.5 1,787,307 + +0.6 1,787,308 - -2.6 1,787,318 + +1.2 1,787,398 - -1.8 1,787,430 - -3.6 1,787,501 + +0.3 1,787,515 + +0.1 1,787,565 - +0.2 1,787,641 - BT1451 0.15 +2.0 1,787,755 - BT1451 0.34 -0.5 1,787,804 - BT1451 0.43 -2.1 1,787,965 - BT1451 0.70 -3.1 1,787,975 + BT1451 0.72 +0.7 1,788,038 + BT1451 0.82 -0.2 1,788,038 + BT1451 0.82 -3.2 1,788,126 + +0.3 1,788,180 - -1.1 1,788,191 + +1.9 1,788,192 - -2.7 1,788,193 + -1.8 1,788,211 + -1.8 1,788,212 - -3.5 1,788,265 + +0.2
Or see this region's nucleotide sequence