Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0217 and BT0218 are separated by 111 nucleotides BT0218 and BT0219 are separated by 14 nucleotides BT0219 and BT0220 are separated by 285 nucleotides BT0220 and BT0221 are separated by 50 nucleotides
BT0217: BT0217 - conserved hypothetical protein (NCBI ptt file), at 239,643 to 240,245
BT0217
BT0218: BT0218 - thioredoxin (TRX) (NCBI ptt file), at 240,357 to 240,821
BT0218
BT0219: BT0219 - thioredoxin C-2 (NCBI ptt file), at 240,836 to 241,135
BT0219
BT0220: BT0220 - hypothetical protein (NCBI ptt file), at 241,421 to 241,606
BT0220
BT0221: BT0221 - conserved hypothetical protein (NCBI ptt file), at 241,657 to 242,916
BT0221
Position (kb) 240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 239.876 kb on - strand, within BT0217 at 239.881 kb on + strand, within BT0217 at 239.881 kb on + strand, within BT0217 at 239.883 kb on + strand, within BT0217 at 239.884 kb on - strand, within BT0217 at 239.908 kb on - strand, within BT0217 at 239.908 kb on - strand, within BT0217 at 239.908 kb on - strand, within BT0217 at 240.107 kb on + strand, within BT0217 at 240.184 kb on + strand, within BT0217 at 240.200 kb on + strand at 240.304 kb on + strand at 240.310 kb on - strand at 240.311 kb on + strand at 240.311 kb on + strand at 240.342 kb on + strand at 240.344 kb on + strand at 240.358 kb on + strand at 240.508 kb on - strand, within BT0218 at 240.542 kb on - strand, within BT0218 at 240.605 kb on - strand, within BT0218 at 240.605 kb on - strand, within BT0218 at 240.958 kb on + strand, within BT0219 at 240.959 kb on - strand, within BT0219 at 240.989 kb on + strand, within BT0219 at 240.990 kb on - strand, within BT0219 at 241.016 kb on + strand, within BT0219 at 241.161 kb on - strand at 241.267 kb on + strand at 241.336 kb on - strand at 241.370 kb on - strand at 241.404 kb on - strand at 241.424 kb on + strand at 241.473 kb on - strand, within BT0220 at 241.484 kb on - strand, within BT0220 at 241.493 kb on - strand, within BT0220 at 241.654 kb on - strand at 241.728 kb on - strand at 241.779 kb on + strand at 241.826 kb on + strand, within BT0221 at 241.869 kb on + strand, within BT0221 at 241.870 kb on - strand, within BT0221 at 241.887 kb on - strand, within BT0221 at 241.921 kb on + strand, within BT0221 at 241.921 kb on + strand, within BT0221 at 241.922 kb on - strand, within BT0221 at 241.923 kb on + strand, within BT0221 at 241.923 kb on + strand, within BT0221 at 241.997 kb on + strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 242.078 kb on + strand, within BT0221 at 242.119 kb on - strand, within BT0221
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 239,876 - BT0217 0.39 +1.1 239,881 + BT0217 0.39 -0.9 239,881 + BT0217 0.39 -1.1 239,883 + BT0217 0.40 +0.1 239,884 - BT0217 0.40 -2.9 239,908 - BT0217 0.44 +1.6 239,908 - BT0217 0.44 +1.5 239,908 - BT0217 0.44 +1.4 240,107 + BT0217 0.77 -2.1 240,184 + BT0217 0.90 -2.8 240,200 + +0.3 240,304 + +0.9 240,310 - -1.1 240,311 + +1.3 240,311 + -1.3 240,342 + -2.1 240,344 + +0.6 240,358 + -1.5 240,508 - BT0218 0.32 -2.3 240,542 - BT0218 0.40 -0.6 240,605 - BT0218 0.53 -2.5 240,605 - BT0218 0.53 -2.2 240,958 + BT0219 0.41 +0.2 240,959 - BT0219 0.41 -0.4 240,989 + BT0219 0.51 -2.2 240,990 - BT0219 0.51 -2.5 241,016 + BT0219 0.60 -1.7 241,161 - -2.8 241,267 + +1.0 241,336 - -3.3 241,370 - -2.8 241,404 - -2.5 241,424 + +2.5 241,473 - BT0220 0.28 -1.1 241,484 - BT0220 0.34 -0.5 241,493 - BT0220 0.39 -1.9 241,654 - -3.2 241,728 - -1.9 241,779 + +0.7 241,826 + BT0221 0.13 -1.8 241,869 + BT0221 0.17 -1.5 241,870 - BT0221 0.17 -2.3 241,887 - BT0221 0.18 +0.8 241,921 + BT0221 0.21 +0.7 241,921 + BT0221 0.21 -3.0 241,922 - BT0221 0.21 -1.8 241,923 + BT0221 0.21 -2.1 241,923 + BT0221 0.21 -1.8 241,997 + BT0221 0.27 -1.6 241,998 - BT0221 0.27 -0.7 241,998 - BT0221 0.27 -0.8 241,998 - BT0221 0.27 +3.8 241,998 - BT0221 0.27 -0.7 242,078 + BT0221 0.33 -1.7 242,119 - BT0221 0.37 +1.1
Or see this region's nucleotide sequence