Experiment: Heparin from porcine intestinal mucosa (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0083 and BT0084 are separated by 103 nucleotides BT0084 and BT0085 are separated by 21 nucleotides BT0085 and BT0086 are separated by 2 nucleotides BT0086 and BT0087 are separated by 33 nucleotides
BT0083: BT0083 - conserved hypothetical protein (NCBI ptt file), at 78,109 to 78,975
BT0083
BT0084: BT0084 - conserved protein found in conjugate transposon (NCBI ptt file), at 79,079 to 79,522
BT0084
BT0085: BT0085 - conserved protein found in conjugate transposon (NCBI ptt file), at 79,544 to 80,122
BT0085
BT0086: BT0086 - conserved protein found in conjugate transposon (NCBI ptt file), at 80,125 to 81,033
BT0086
BT0087: BT0087 - conserved protein found in conjugate transposon (NCBI ptt file), at 81,067 to 82,452
BT0087
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 78.546 kb on - strand, within BT0083 at 78.556 kb on - strand, within BT0083 at 78.559 kb on - strand, within BT0083 at 78.575 kb on + strand, within BT0083 at 78.577 kb on + strand, within BT0083 at 78.578 kb on - strand, within BT0083 at 78.593 kb on - strand, within BT0083 at 78.613 kb on + strand, within BT0083 at 78.613 kb on + strand, within BT0083 at 78.622 kb on + strand, within BT0083 at 78.690 kb on - strand, within BT0083 at 78.692 kb on + strand, within BT0083 at 78.692 kb on + strand, within BT0083 at 78.733 kb on + strand, within BT0083 at 78.733 kb on + strand, within BT0083 at 78.733 kb on + strand, within BT0083 at 78.734 kb on - strand, within BT0083 at 78.763 kb on + strand, within BT0083 at 78.775 kb on + strand, within BT0083 at 78.776 kb on - strand, within BT0083 at 78.792 kb on + strand, within BT0083 at 78.792 kb on + strand, within BT0083 at 78.830 kb on + strand, within BT0083 at 78.831 kb on - strand, within BT0083 at 78.841 kb on - strand, within BT0083 at 79.099 kb on + strand at 79.120 kb on + strand at 79.164 kb on - strand, within BT0084 at 79.297 kb on - strand, within BT0084 at 79.430 kb on - strand, within BT0084 at 79.475 kb on + strand, within BT0084 at 79.503 kb on - strand at 79.503 kb on - strand at 79.517 kb on - strand at 79.519 kb on - strand at 79.523 kb on + strand at 79.526 kb on - strand at 79.537 kb on + strand at 79.559 kb on + strand at 79.656 kb on + strand, within BT0085 at 79.866 kb on - strand, within BT0085 at 79.881 kb on + strand, within BT0085 at 79.881 kb on + strand, within BT0085 at 79.902 kb on + strand, within BT0085 at 79.941 kb on + strand, within BT0085 at 79.945 kb on + strand, within BT0085 at 79.985 kb on - strand, within BT0085 at 80.101 kb on + strand at 80.111 kb on + strand at 80.234 kb on - strand, within BT0086 at 80.270 kb on + strand, within BT0086 at 80.271 kb on - strand, within BT0086 at 80.271 kb on - strand, within BT0086 at 80.317 kb on - strand, within BT0086 at 80.472 kb on - strand, within BT0086 at 80.570 kb on - strand, within BT0086 at 80.709 kb on + strand, within BT0086 at 80.779 kb on - strand, within BT0086 at 80.831 kb on + strand, within BT0086 at 80.877 kb on + strand, within BT0086 at 80.881 kb on + strand, within BT0086 at 80.906 kb on - strand, within BT0086 at 80.913 kb on - strand, within BT0086 at 81.068 kb on + strand at 81.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Heparin from porcine intestinal mucosa (C) remove 78,546 - BT0083 0.50 +0.3 78,556 - BT0083 0.52 +0.5 78,559 - BT0083 0.52 +0.7 78,575 + BT0083 0.54 +0.6 78,577 + BT0083 0.54 -0.4 78,578 - BT0083 0.54 -0.5 78,593 - BT0083 0.56 +0.1 78,613 + BT0083 0.58 -2.7 78,613 + BT0083 0.58 +0.7 78,622 + BT0083 0.59 +0.6 78,690 - BT0083 0.67 -3.1 78,692 + BT0083 0.67 -0.5 78,692 + BT0083 0.67 +1.1 78,733 + BT0083 0.72 +0.5 78,733 + BT0083 0.72 +3.1 78,733 + BT0083 0.72 -1.4 78,734 - BT0083 0.72 -3.5 78,763 + BT0083 0.75 -2.3 78,775 + BT0083 0.77 +0.7 78,776 - BT0083 0.77 -0.4 78,792 + BT0083 0.79 -1.8 78,792 + BT0083 0.79 +0.1 78,830 + BT0083 0.83 -4.8 78,831 - BT0083 0.83 -2.2 78,841 - BT0083 0.84 -0.5 79,099 + +1.7 79,120 + +1.1 79,164 - BT0084 0.19 +1.5 79,297 - BT0084 0.49 -3.3 79,430 - BT0084 0.79 -0.5 79,475 + BT0084 0.89 -0.5 79,503 - -2.1 79,503 - -1.4 79,517 - -1.1 79,519 - -0.3 79,523 + -2.3 79,526 - -0.7 79,537 + +0.5 79,559 + -3.4 79,656 + BT0085 0.19 -1.5 79,866 - BT0085 0.56 -2.5 79,881 + BT0085 0.58 -2.2 79,881 + BT0085 0.58 -0.9 79,902 + BT0085 0.62 -3.0 79,941 + BT0085 0.69 -1.1 79,945 + BT0085 0.69 -0.5 79,985 - BT0085 0.76 -3.0 80,101 + +1.6 80,111 + -1.1 80,234 - BT0086 0.12 -3.1 80,270 + BT0086 0.16 -2.3 80,271 - BT0086 0.16 -1.5 80,271 - BT0086 0.16 -3.1 80,317 - BT0086 0.21 +1.1 80,472 - BT0086 0.38 -2.3 80,570 - BT0086 0.49 -1.8 80,709 + BT0086 0.64 -1.5 80,779 - BT0086 0.72 -1.3 80,831 + BT0086 0.78 -1.3 80,877 + BT0086 0.83 -0.5 80,881 + BT0086 0.83 -0.0 80,906 - BT0086 0.86 -0.4 80,913 - BT0086 0.87 -2.3 81,068 + -2.3 81,121 + -2.2
Or see this region's nucleotide sequence