Experiment: L-Malic Acid (C) with ammonium chloride; high light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1793 and Rru_A1794 overlap by 4 nucleotides Rru_A1794 and Rru_A1795 are separated by 114 nucleotides Rru_A1795 and Rru_A1796 are separated by 12 nucleotides
Rru_A1793: Rru_A1793 - Secretion protein HlyD (NCBI), at 2,085,290 to 2,086,333
_A1793
Rru_A1794: Rru_A1794 - Transcriptional Regulator, TetR family (NCBI), at 2,086,330 to 2,087,019
_A1794
Rru_A1795: Rru_A1795 - HAD-superfamily hydrolase, subfamily IIA (NCBI), at 2,087,134 to 2,088,048
_A1795
Rru_A1796: Rru_A1796 - Valyl-tRNA synthetase, class Ia (NCBI), at 2,088,061 to 2,090,700
_A1796
Position (kb)
2087
2088
2089 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2086.210 kb on - strand, within Rru_A1793 at 2086.586 kb on + strand, within Rru_A1794 at 2086.663 kb on + strand, within Rru_A1794 at 2086.836 kb on - strand, within Rru_A1794 at 2086.859 kb on + strand, within Rru_A1794 at 2086.859 kb on + strand, within Rru_A1794 at 2086.860 kb on - strand, within Rru_A1794 at 2086.860 kb on - strand, within Rru_A1794 at 2087.269 kb on - strand, within Rru_A1795 at 2087.269 kb on - strand, within Rru_A1795 at 2087.339 kb on + strand, within Rru_A1795 at 2087.339 kb on + strand, within Rru_A1795 at 2087.339 kb on + strand, within Rru_A1795 at 2087.339 kb on + strand, within Rru_A1795 at 2087.340 kb on - strand, within Rru_A1795 at 2087.340 kb on - strand, within Rru_A1795 at 2087.340 kb on - strand, within Rru_A1795 at 2087.352 kb on - strand, within Rru_A1795 at 2087.894 kb on + strand, within Rru_A1795 at 2087.895 kb on - strand, within Rru_A1795 at 2088.011 kb on + strand at 2088.011 kb on + strand at 2088.011 kb on + strand at 2088.012 kb on - strand at 2088.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; high light remove 2,086,210 - Rru_A1793 0.88 -0.3 2,086,586 + Rru_A1794 0.37 +0.3 2,086,663 + Rru_A1794 0.48 +0.4 2,086,836 - Rru_A1794 0.73 +0.1 2,086,859 + Rru_A1794 0.77 +1.1 2,086,859 + Rru_A1794 0.77 -0.4 2,086,860 - Rru_A1794 0.77 +0.5 2,086,860 - Rru_A1794 0.77 -0.1 2,087,269 - Rru_A1795 0.15 -0.1 2,087,269 - Rru_A1795 0.15 +0.8 2,087,339 + Rru_A1795 0.22 -0.2 2,087,339 + Rru_A1795 0.22 +0.4 2,087,339 + Rru_A1795 0.22 +0.4 2,087,339 + Rru_A1795 0.22 -0.6 2,087,340 - Rru_A1795 0.23 -1.6 2,087,340 - Rru_A1795 0.23 +0.4 2,087,340 - Rru_A1795 0.23 +0.9 2,087,352 - Rru_A1795 0.24 +0.5 2,087,894 + Rru_A1795 0.83 -0.1 2,087,895 - Rru_A1795 0.83 +0.4 2,088,011 + -3.3 2,088,011 + +1.7 2,088,011 + -0.1 2,088,012 - -0.2 2,088,012 - +0.4
Or see this region's nucleotide sequence