Experiment: L-Malic Acid (C) with ammonium chloride; high light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0909 and Rru_A0910 are separated by 40 nucleotides Rru_A0910 and Rru_A0911 overlap by 4 nucleotides Rru_A0911 and Rru_A0912 are separated by 13 nucleotides Rru_A0912 and Rru_A0913 are separated by 10 nucleotides Rru_A0913 and Rru_A0914 overlap by 1 nucleotides
Rru_A0909: Rru_A0909 - Propanediol utilization protein (NCBI), at 1,084,367 to 1,085,095
_A0909
Rru_A0910: Rru_A0910 - ethanolamine utilization protein eutJ (NCBI), at 1,085,136 to 1,085,987
_A0910
Rru_A0911: Rru_A0911 - hypothetical protein (NCBI), at 1,085,984 to 1,086,826
_A0911
Rru_A0912: Rru_A0912 - Ethanolamine utilization protein EutN/carboxysome structural protein Ccml (NCBI), at 1,086,840 to 1,087,109
_A0912
Rru_A0913: Rru_A0913 - Protein of unknown function DUF336 (NCBI), at 1,087,120 to 1,087,641
_A0913
Rru_A0914: Rru_A0914 - Aldehyde dehydrogenase (NCBI), at 1,087,641 to 1,089,230
_A0914
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1085.015 kb on + strand, within Rru_A0909 at 1085.015 kb on + strand, within Rru_A0909 at 1085.016 kb on - strand, within Rru_A0909 at 1085.016 kb on - strand, within Rru_A0909 at 1085.026 kb on + strand at 1085.026 kb on + strand at 1085.027 kb on - strand at 1085.027 kb on - strand at 1085.256 kb on - strand, within Rru_A0910 at 1085.264 kb on + strand, within Rru_A0910 at 1085.264 kb on + strand, within Rru_A0910 at 1085.355 kb on + strand, within Rru_A0910 at 1085.355 kb on + strand, within Rru_A0910 at 1085.356 kb on - strand, within Rru_A0910 at 1085.610 kb on - strand, within Rru_A0910 at 1085.666 kb on + strand, within Rru_A0910 at 1085.667 kb on - strand, within Rru_A0910 at 1085.667 kb on - strand, within Rru_A0910 at 1085.817 kb on + strand, within Rru_A0910 at 1085.835 kb on + strand, within Rru_A0910 at 1085.835 kb on + strand, within Rru_A0910 at 1085.835 kb on + strand, within Rru_A0910 at 1085.836 kb on - strand, within Rru_A0910 at 1085.836 kb on - strand, within Rru_A0910 at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.955 kb on + strand at 1085.983 kb on + strand at 1086.119 kb on - strand, within Rru_A0911 at 1086.199 kb on + strand, within Rru_A0911 at 1086.476 kb on + strand, within Rru_A0911 at 1086.476 kb on + strand, within Rru_A0911 at 1086.479 kb on - strand, within Rru_A0911 at 1086.518 kb on + strand, within Rru_A0911 at 1086.613 kb on - strand, within Rru_A0911 at 1086.629 kb on - strand, within Rru_A0911 at 1086.824 kb on + strand at 1086.824 kb on + strand at 1086.824 kb on + strand at 1086.824 kb on + strand at 1086.825 kb on - strand at 1086.843 kb on + strand at 1086.844 kb on - strand at 1086.844 kb on - strand at 1086.844 kb on - strand at 1086.844 kb on - strand at 1086.844 kb on - strand at 1086.844 kb on - strand at 1087.107 kb on + strand at 1087.451 kb on - strand, within Rru_A0913 at 1087.689 kb on + strand at 1087.690 kb on - strand at 1087.690 kb on - strand at 1087.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; high light remove 1,085,015 + Rru_A0909 0.89 -1.6 1,085,015 + Rru_A0909 0.89 -0.8 1,085,016 - Rru_A0909 0.89 -0.8 1,085,016 - Rru_A0909 0.89 -0.2 1,085,026 + -0.9 1,085,026 + -0.4 1,085,027 - -1.0 1,085,027 - +0.4 1,085,256 - Rru_A0910 0.14 +0.4 1,085,264 + Rru_A0910 0.15 -0.1 1,085,264 + Rru_A0910 0.15 -0.4 1,085,355 + Rru_A0910 0.26 -0.5 1,085,355 + Rru_A0910 0.26 -0.0 1,085,356 - Rru_A0910 0.26 -0.3 1,085,610 - Rru_A0910 0.56 -0.2 1,085,666 + Rru_A0910 0.62 +0.1 1,085,667 - Rru_A0910 0.62 -0.5 1,085,667 - Rru_A0910 0.62 -0.4 1,085,817 + Rru_A0910 0.80 -0.4 1,085,835 + Rru_A0910 0.82 -0.5 1,085,835 + Rru_A0910 0.82 -0.9 1,085,835 + Rru_A0910 0.82 -0.7 1,085,836 - Rru_A0910 0.82 +0.1 1,085,836 - Rru_A0910 0.82 -0.1 1,085,955 + -0.8 1,085,955 + -0.9 1,085,955 + -1.7 1,085,955 + +0.6 1,085,955 + +0.1 1,085,955 + -1.5 1,085,955 + +0.4 1,085,983 + -0.1 1,086,119 - Rru_A0911 0.16 -0.6 1,086,199 + Rru_A0911 0.26 -0.3 1,086,476 + Rru_A0911 0.58 +0.4 1,086,476 + Rru_A0911 0.58 -0.3 1,086,479 - Rru_A0911 0.59 -0.5 1,086,518 + Rru_A0911 0.63 -0.9 1,086,613 - Rru_A0911 0.75 -0.1 1,086,629 - Rru_A0911 0.77 -1.0 1,086,824 + -2.9 1,086,824 + +0.7 1,086,824 + -1.2 1,086,824 + -1.6 1,086,825 - -0.2 1,086,843 + +0.4 1,086,844 - -0.6 1,086,844 - +1.0 1,086,844 - +0.3 1,086,844 - +0.2 1,086,844 - +0.2 1,086,844 - -0.1 1,087,107 + -1.3 1,087,451 - Rru_A0913 0.63 +0.0 1,087,689 + -1.4 1,087,690 - +0.6 1,087,690 - -0.2 1,087,690 - -0.3
Or see this region's nucleotide sequence