Experiment: R2A with Calcium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS16155 and LRK54_RS16160 overlap by 2 nucleotides LRK54_RS16160 and LRK54_RS16165 are separated by 87 nucleotides LRK54_RS16165 and LRK54_RS16170 overlap by 4 nucleotides LRK54_RS16170 and LRK54_RS16175 are separated by 4 nucleotides
LRK54_RS16155: LRK54_RS16155 - folate-binding protein, at 3,555,590 to 3,556,525
_RS16155
LRK54_RS16160: LRK54_RS16160 - DUF1674 domain-containing protein, at 3,556,524 to 3,556,691
_RS16160
LRK54_RS16165: LRK54_RS16165 - succinate dehydrogenase, cytochrome b556 subunit, at 3,556,779 to 3,557,174
_RS16165
LRK54_RS16170: LRK54_RS16170 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 3,557,171 to 3,557,584
_RS16170
LRK54_RS16175: LRK54_RS16175 - succinate dehydrogenase flavoprotein subunit, at 3,557,589 to 3,559,376
_RS16175
Position (kb)
3556
3557
3558 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3556.206 kb on + strand, within LRK54_RS16155 at 3556.207 kb on - strand, within LRK54_RS16155 at 3556.207 kb on - strand, within LRK54_RS16155 at 3556.356 kb on + strand, within LRK54_RS16155 at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.453 kb on + strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.454 kb on - strand at 3556.478 kb on + strand at 3556.478 kb on + strand at 3556.478 kb on + strand at 3556.478 kb on + strand at 3556.478 kb on + strand at 3556.478 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.495 kb on + strand at 3556.498 kb on + strand at 3556.498 kb on + strand at 3556.498 kb on + strand at 3556.498 kb on + strand at 3556.532 kb on + strand at 3556.532 kb on + strand at 3556.532 kb on + strand at 3556.532 kb on + strand at 3556.532 kb on + strand at 3556.615 kb on + strand, within LRK54_RS16160 at 3556.679 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Calcium chloride 125 mM remove 3,556,206 + LRK54_RS16155 0.66 -1.6 3,556,207 - LRK54_RS16155 0.66 -1.2 3,556,207 - LRK54_RS16155 0.66 -1.7 3,556,356 + LRK54_RS16155 0.82 -1.1 3,556,453 + -0.9 3,556,453 + -0.8 3,556,453 + -1.0 3,556,453 + -0.0 3,556,453 + +1.4 3,556,453 + -3.1 3,556,453 + +0.6 3,556,454 - -2.2 3,556,454 - +1.6 3,556,454 - -2.7 3,556,454 - -2.4 3,556,454 - -0.4 3,556,454 - -1.6 3,556,454 - -2.2 3,556,454 - +0.6 3,556,478 + -0.6 3,556,478 + -0.2 3,556,478 + -0.4 3,556,478 + -0.9 3,556,478 + -1.4 3,556,478 + -1.9 3,556,495 + +0.6 3,556,495 + +0.6 3,556,495 + -0.4 3,556,495 + -1.2 3,556,495 + -1.0 3,556,495 + -1.0 3,556,495 + +0.3 3,556,495 + -0.7 3,556,495 + -1.4 3,556,495 + -1.7 3,556,498 + +0.6 3,556,498 + -1.6 3,556,498 + -1.3 3,556,498 + -1.1 3,556,532 + -0.8 3,556,532 + +0.5 3,556,532 + -0.8 3,556,532 + -0.7 3,556,532 + -2.6 3,556,615 + LRK54_RS16160 0.54 -0.2 3,556,679 + +0.1
Or see this region's nucleotide sequence