Experiment: R2A with Calcium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04910 and LRK54_RS04915 overlap by 4 nucleotides LRK54_RS04915 and LRK54_RS04920 are separated by 139 nucleotides LRK54_RS04920 and LRK54_RS04925 are separated by 98 nucleotides LRK54_RS04925 and LRK54_RS04930 are separated by 40 nucleotides
LRK54_RS04910: LRK54_RS04910 - Holliday junction branch migration protein RuvA, at 1,041,100 to 1,041,696
_RS04910
LRK54_RS04915: LRK54_RS04915 - crossover junction endodeoxyribonuclease RuvC, at 1,041,693 to 1,042,211
_RS04915
LRK54_RS04920: LRK54_RS04920 - YebC/PmpR family DNA-binding transcriptional regulator, at 1,042,351 to 1,043,079
_RS04920
LRK54_RS04925: LRK54_RS04925 - alpha/beta hydrolase, at 1,043,178 to 1,043,813
_RS04925
LRK54_RS04930: LRK54_RS04930 - aspartate--tRNA ligase, at 1,043,854 to 1,045,626
_RS04930
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1041.591 kb on - strand, within LRK54_RS04910 at 1041.905 kb on - strand, within LRK54_RS04915 at 1041.905 kb on - strand, within LRK54_RS04915 at 1041.905 kb on - strand, within LRK54_RS04915 at 1041.905 kb on - strand, within LRK54_RS04915 at 1042.318 kb on + strand at 1042.318 kb on + strand at 1042.318 kb on + strand at 1042.319 kb on - strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.393 kb on + strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.394 kb on - strand at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.713 kb on + strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.714 kb on - strand, within LRK54_RS04920 at 1042.745 kb on + strand, within LRK54_RS04920 at 1042.745 kb on + strand, within LRK54_RS04920 at 1042.745 kb on + strand, within LRK54_RS04920 at 1042.746 kb on - strand, within LRK54_RS04920 at 1042.764 kb on + strand, within LRK54_RS04920 at 1042.765 kb on - strand, within LRK54_RS04920 at 1042.830 kb on - strand, within LRK54_RS04920 at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.073 kb on + strand at 1043.074 kb on - strand at 1043.704 kb on + strand, within LRK54_RS04925 at 1043.704 kb on + strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.705 kb on - strand, within LRK54_RS04925 at 1043.819 kb on - strand at 1043.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Calcium chloride 125 mM remove 1,041,591 - LRK54_RS04910 0.82 +2.1 1,041,905 - LRK54_RS04915 0.41 -0.1 1,041,905 - LRK54_RS04915 0.41 -0.8 1,041,905 - LRK54_RS04915 0.41 -0.8 1,041,905 - LRK54_RS04915 0.41 +0.9 1,042,318 + +0.5 1,042,318 + -0.3 1,042,318 + +0.9 1,042,319 - -0.1 1,042,393 + -0.8 1,042,393 + -3.2 1,042,393 + -3.3 1,042,393 + -1.7 1,042,393 + -2.9 1,042,393 + -0.5 1,042,393 + -3.2 1,042,393 + -1.7 1,042,393 + -3.5 1,042,393 + -1.7 1,042,393 + -2.2 1,042,393 + +0.5 1,042,393 + -0.8 1,042,393 + -1.8 1,042,393 + -2.4 1,042,394 - -2.2 1,042,394 - -2.3 1,042,394 - -0.2 1,042,394 - -1.8 1,042,394 - -1.1 1,042,394 - -0.4 1,042,394 - +1.1 1,042,394 - -0.9 1,042,394 - -1.8 1,042,394 - -1.3 1,042,394 - -0.1 1,042,713 + LRK54_RS04920 0.50 -3.3 1,042,713 + LRK54_RS04920 0.50 -1.5 1,042,713 + LRK54_RS04920 0.50 +0.2 1,042,713 + LRK54_RS04920 0.50 -0.8 1,042,713 + LRK54_RS04920 0.50 -0.5 1,042,713 + LRK54_RS04920 0.50 +1.5 1,042,713 + LRK54_RS04920 0.50 -4.2 1,042,713 + LRK54_RS04920 0.50 +0.5 1,042,713 + LRK54_RS04920 0.50 -0.1 1,042,713 + LRK54_RS04920 0.50 -0.5 1,042,713 + LRK54_RS04920 0.50 +0.9 1,042,713 + LRK54_RS04920 0.50 -1.9 1,042,713 + LRK54_RS04920 0.50 -1.8 1,042,713 + LRK54_RS04920 0.50 -2.6 1,042,713 + LRK54_RS04920 0.50 -1.8 1,042,714 - LRK54_RS04920 0.50 -2.9 1,042,714 - LRK54_RS04920 0.50 -1.9 1,042,714 - LRK54_RS04920 0.50 -2.7 1,042,714 - LRK54_RS04920 0.50 -1.5 1,042,714 - LRK54_RS04920 0.50 -2.1 1,042,714 - LRK54_RS04920 0.50 -1.7 1,042,714 - LRK54_RS04920 0.50 -1.7 1,042,714 - LRK54_RS04920 0.50 -3.2 1,042,714 - LRK54_RS04920 0.50 -1.2 1,042,714 - LRK54_RS04920 0.50 -0.8 1,042,714 - LRK54_RS04920 0.50 -3.1 1,042,714 - LRK54_RS04920 0.50 -0.1 1,042,714 - LRK54_RS04920 0.50 -2.7 1,042,714 - LRK54_RS04920 0.50 -3.5 1,042,714 - LRK54_RS04920 0.50 -2.9 1,042,714 - LRK54_RS04920 0.50 -0.4 1,042,714 - LRK54_RS04920 0.50 -2.5 1,042,714 - LRK54_RS04920 0.50 +0.5 1,042,714 - LRK54_RS04920 0.50 -0.5 1,042,714 - LRK54_RS04920 0.50 -4.9 1,042,745 + LRK54_RS04920 0.54 -0.8 1,042,745 + LRK54_RS04920 0.54 -1.7 1,042,745 + LRK54_RS04920 0.54 -2.3 1,042,746 - LRK54_RS04920 0.54 -2.3 1,042,764 + LRK54_RS04920 0.57 -2.9 1,042,765 - LRK54_RS04920 0.57 -1.8 1,042,830 - LRK54_RS04920 0.66 -1.7 1,043,073 + +0.5 1,043,073 + -0.5 1,043,073 + -2.8 1,043,073 + -2.3 1,043,073 + -1.2 1,043,073 + -3.0 1,043,073 + -3.1 1,043,074 - -3.2 1,043,704 + LRK54_RS04925 0.83 -0.4 1,043,704 + LRK54_RS04925 0.83 +0.4 1,043,705 - LRK54_RS04925 0.83 -0.1 1,043,705 - LRK54_RS04925 0.83 -0.3 1,043,705 - LRK54_RS04925 0.83 +0.4 1,043,705 - LRK54_RS04925 0.83 +0.5 1,043,705 - LRK54_RS04925 0.83 +0.3 1,043,705 - LRK54_RS04925 0.83 +1.0 1,043,705 - LRK54_RS04925 0.83 +2.3 1,043,819 - -0.6 1,043,819 - +0.4
Or see this region's nucleotide sequence