Experiment: PYE with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01714 and CCNA_01715 are separated by 105 nucleotides CCNA_01715 and CCNA_01716 are separated by 36 nucleotides CCNA_01716 and CCNA_01717 are separated by 12 nucleotides CCNA_01717 and CCNA_01718 overlap by 1 nucleotides CCNA_01718 and CCNA_01719 overlap by 4 nucleotides
CCNA_01714: CCNA_01714 - Spx/MgsR-related protein, at 1,845,426 to 1,845,773
_01714
CCNA_01715: CCNA_01715 - GPH-family phosphatase, at 1,845,879 to 1,846,565
_01715
CCNA_01716: CCNA_01716 - hypothetical protein, at 1,846,602 to 1,846,787
_01716
CCNA_01717: CCNA_01717 - hypothetical protein, at 1,846,800 to 1,847,210
_01717
CCNA_01718: CCNA_01718 - RNA polymerase ECF sigma factor, at 1,847,210 to 1,847,725
_01718
CCNA_01719: CCNA_01719 - hypothetical protein, at 1,847,722 to 1,848,030
_01719
Position (kb)
1846
1847 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1845.623 kb on + strand, within CCNA_01714 at 1845.705 kb on + strand, within CCNA_01714 at 1845.705 kb on + strand, within CCNA_01714 at 1845.706 kb on + strand, within CCNA_01714 at 1845.706 kb on + strand, within CCNA_01714 at 1845.706 kb on + strand, within CCNA_01714 at 1845.706 kb on + strand, within CCNA_01714 at 1845.706 kb on + strand, within CCNA_01714 at 1845.713 kb on - strand, within CCNA_01714 at 1845.713 kb on - strand, within CCNA_01714 at 1845.714 kb on - strand, within CCNA_01714 at 1845.782 kb on + strand at 1845.841 kb on + strand at 1845.853 kb on + strand at 1845.874 kb on + strand at 1845.882 kb on - strand at 1845.900 kb on + strand at 1845.934 kb on + strand at 1845.977 kb on - strand, within CCNA_01715 at 1846.069 kb on + strand, within CCNA_01715 at 1846.077 kb on - strand, within CCNA_01715 at 1846.077 kb on - strand, within CCNA_01715 at 1846.141 kb on - strand, within CCNA_01715 at 1846.158 kb on + strand, within CCNA_01715 at 1846.166 kb on + strand, within CCNA_01715 at 1846.187 kb on + strand, within CCNA_01715 at 1846.267 kb on + strand, within CCNA_01715 at 1846.299 kb on - strand, within CCNA_01715 at 1846.300 kb on - strand, within CCNA_01715 at 1846.316 kb on + strand, within CCNA_01715 at 1846.343 kb on + strand, within CCNA_01715 at 1846.426 kb on + strand, within CCNA_01715 at 1846.715 kb on + strand, within CCNA_01716 at 1846.723 kb on - strand, within CCNA_01716 at 1846.775 kb on - strand at 1846.800 kb on + strand at 1846.817 kb on + strand at 1846.866 kb on + strand, within CCNA_01717 at 1846.866 kb on + strand, within CCNA_01717 at 1846.874 kb on - strand, within CCNA_01717 at 1846.902 kb on + strand, within CCNA_01717 at 1846.903 kb on + strand, within CCNA_01717 at 1846.903 kb on + strand, within CCNA_01717 at 1846.903 kb on + strand, within CCNA_01717 at 1846.903 kb on + strand, within CCNA_01717 at 1846.910 kb on - strand, within CCNA_01717 at 1846.911 kb on - strand, within CCNA_01717 at 1846.911 kb on - strand, within CCNA_01717 at 1846.913 kb on + strand, within CCNA_01717 at 1846.913 kb on + strand, within CCNA_01717 at 1846.913 kb on + strand, within CCNA_01717 at 1846.928 kb on - strand, within CCNA_01717 at 1846.956 kb on + strand, within CCNA_01717 at 1846.956 kb on + strand, within CCNA_01717 at 1846.956 kb on + strand, within CCNA_01717 at 1846.956 kb on + strand, within CCNA_01717 at 1846.956 kb on + strand, within CCNA_01717 at 1847.016 kb on + strand, within CCNA_01717 at 1847.046 kb on - strand, within CCNA_01717 at 1847.102 kb on + strand, within CCNA_01717 at 1847.115 kb on + strand, within CCNA_01717 at 1847.183 kb on + strand at 1847.224 kb on + strand at 1847.224 kb on + strand at 1847.232 kb on - strand at 1847.266 kb on + strand, within CCNA_01718 at 1847.349 kb on + strand, within CCNA_01718 at 1847.410 kb on + strand, within CCNA_01718 at 1847.410 kb on + strand, within CCNA_01718 at 1847.421 kb on - strand, within CCNA_01718 at 1847.434 kb on + strand, within CCNA_01718 at 1847.436 kb on + strand, within CCNA_01718 at 1847.476 kb on + strand, within CCNA_01718 at 1847.482 kb on + strand, within CCNA_01718 at 1847.511 kb on - strand, within CCNA_01718 at 1847.589 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.606 kb on + strand, within CCNA_01718 at 1847.612 kb on + strand, within CCNA_01718 at 1847.614 kb on - strand, within CCNA_01718 at 1847.614 kb on - strand, within CCNA_01718 at 1847.616 kb on + strand, within CCNA_01718 at 1847.629 kb on + strand, within CCNA_01718 at 1847.629 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.705 kb on + strand at 1847.713 kb on - strand at 1847.739 kb on + strand at 1847.787 kb on + strand, within CCNA_01719
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Doxycycline hyclate 0.0001 mg/ml remove 1,845,623 + CCNA_01714 0.57 -0.6 1,845,705 + CCNA_01714 0.80 +0.2 1,845,705 + CCNA_01714 0.80 +0.1 1,845,706 + CCNA_01714 0.80 -0.5 1,845,706 + CCNA_01714 0.80 -0.3 1,845,706 + CCNA_01714 0.80 +0.7 1,845,706 + CCNA_01714 0.80 +1.0 1,845,706 + CCNA_01714 0.80 +0.2 1,845,713 - CCNA_01714 0.82 +0.2 1,845,713 - CCNA_01714 0.82 -0.6 1,845,714 - CCNA_01714 0.83 -1.9 1,845,782 + +0.4 1,845,841 + -0.2 1,845,853 + +0.3 1,845,874 + +0.5 1,845,882 - +1.0 1,845,900 + -0.2 1,845,934 + +0.6 1,845,977 - CCNA_01715 0.14 +3.0 1,846,069 + CCNA_01715 0.28 -0.1 1,846,077 - CCNA_01715 0.29 -0.0 1,846,077 - CCNA_01715 0.29 -0.8 1,846,141 - CCNA_01715 0.38 +0.4 1,846,158 + CCNA_01715 0.41 -0.9 1,846,166 + CCNA_01715 0.42 +0.1 1,846,187 + CCNA_01715 0.45 +0.7 1,846,267 + CCNA_01715 0.56 -0.5 1,846,299 - CCNA_01715 0.61 -0.6 1,846,300 - CCNA_01715 0.61 -1.0 1,846,316 + CCNA_01715 0.64 +1.4 1,846,343 + CCNA_01715 0.68 -0.6 1,846,426 + CCNA_01715 0.80 +1.2 1,846,715 + CCNA_01716 0.61 +1.6 1,846,723 - CCNA_01716 0.65 -1.7 1,846,775 - -1.3 1,846,800 + +3.6 1,846,817 + +1.1 1,846,866 + CCNA_01717 0.16 +0.4 1,846,866 + CCNA_01717 0.16 +2.0 1,846,874 - CCNA_01717 0.18 -0.8 1,846,902 + CCNA_01717 0.25 +1.7 1,846,903 + CCNA_01717 0.25 -1.2 1,846,903 + CCNA_01717 0.25 -1.8 1,846,903 + CCNA_01717 0.25 -0.1 1,846,903 + CCNA_01717 0.25 -0.6 1,846,910 - CCNA_01717 0.27 -0.6 1,846,911 - CCNA_01717 0.27 -1.8 1,846,911 - CCNA_01717 0.27 -2.7 1,846,913 + CCNA_01717 0.27 +0.4 1,846,913 + CCNA_01717 0.27 +2.6 1,846,913 + CCNA_01717 0.27 -0.1 1,846,928 - CCNA_01717 0.31 +1.0 1,846,956 + CCNA_01717 0.38 +1.7 1,846,956 + CCNA_01717 0.38 +0.2 1,846,956 + CCNA_01717 0.38 +2.3 1,846,956 + CCNA_01717 0.38 +1.6 1,846,956 + CCNA_01717 0.38 -0.8 1,847,016 + CCNA_01717 0.53 +0.9 1,847,046 - CCNA_01717 0.60 -0.8 1,847,102 + CCNA_01717 0.73 -0.5 1,847,115 + CCNA_01717 0.77 +1.0 1,847,183 + +1.7 1,847,224 + +1.5 1,847,224 + -0.5 1,847,232 - +0.1 1,847,266 + CCNA_01718 0.11 -0.4 1,847,349 + CCNA_01718 0.27 +0.8 1,847,410 + CCNA_01718 0.39 -0.1 1,847,410 + CCNA_01718 0.39 +0.5 1,847,421 - CCNA_01718 0.41 -0.6 1,847,434 + CCNA_01718 0.43 -0.0 1,847,436 + CCNA_01718 0.44 -0.1 1,847,476 + CCNA_01718 0.52 -0.3 1,847,482 + CCNA_01718 0.53 -0.1 1,847,511 - CCNA_01718 0.58 -0.0 1,847,589 + CCNA_01718 0.73 -0.8 1,847,601 + +1.0 1,847,601 + CCNA_01718 0.76 -0.3 1,847,601 + CCNA_01718 0.76 +2.6 1,847,606 + CCNA_01718 0.77 -0.3 1,847,612 + CCNA_01718 0.78 -0.9 1,847,614 - CCNA_01718 0.78 -0.3 1,847,614 - CCNA_01718 0.78 +0.5 1,847,616 + CCNA_01718 0.79 -0.0 1,847,629 + CCNA_01718 0.81 +0.4 1,847,629 + CCNA_01718 0.81 -0.6 1,847,651 + CCNA_01718 0.85 -1.2 1,847,651 + CCNA_01718 0.85 -0.8 1,847,651 + CCNA_01718 0.85 -0.5 1,847,651 + CCNA_01718 0.85 +2.0 1,847,651 + CCNA_01718 0.85 -0.3 1,847,651 + CCNA_01718 0.85 -0.6 1,847,651 + CCNA_01718 0.85 +2.0 1,847,651 + CCNA_01718 0.85 +1.5 1,847,651 + CCNA_01718 0.85 +1.0 1,847,651 + CCNA_01718 0.85 -0.6 1,847,651 + CCNA_01718 0.85 -1.3 1,847,659 - CCNA_01718 0.87 -0.8 1,847,659 - CCNA_01718 0.87 +1.0 1,847,659 - CCNA_01718 0.87 -0.8 1,847,659 - CCNA_01718 0.87 -0.0 1,847,659 - CCNA_01718 0.87 -1.9 1,847,659 - CCNA_01718 0.87 +0.4 1,847,659 - CCNA_01718 0.87 +0.4 1,847,659 - CCNA_01718 0.87 +0.2 1,847,659 - CCNA_01718 0.87 -0.0 1,847,705 + -1.3 1,847,713 - -2.0 1,847,739 + -1.7 1,847,787 + CCNA_01719 0.21 +0.8
Or see this region's nucleotide sequence