Experiment: PYE with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00255 and CCNA_00256 are separated by 18 nucleotides CCNA_00256 and CCNA_00257 are separated by 118 nucleotides CCNA_00257 and CCNA_00259 are separated by 342 nucleotides
CCNA_00255: CCNA_00255 - esterase, at 268,063 to 269,187
_00255
CCNA_00256: CCNA_00256 - pyridoxine kinase, at 269,206 to 270,057
_00256
CCNA_00257: CCNA_00257 - adenosylhomocysteinase, at 270,176 to 271,567
_00257
CCNA_00259: CCNA_00259 - hypothetical protein, at 271,910 to 272,620
_00259
Position (kb) 270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 269.211 kb on - strand at 269.212 kb on - strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.312 kb on + strand, within CCNA_00256 at 269.320 kb on - strand, within CCNA_00256 at 269.356 kb on + strand, within CCNA_00256 at 269.388 kb on - strand, within CCNA_00256 at 269.429 kb on + strand, within CCNA_00256 at 269.433 kb on - strand, within CCNA_00256 at 269.437 kb on - strand, within CCNA_00256 at 269.437 kb on - strand, within CCNA_00256 at 269.446 kb on + strand, within CCNA_00256 at 269.454 kb on - strand, within CCNA_00256 at 269.467 kb on + strand, within CCNA_00256 at 269.467 kb on + strand, within CCNA_00256 at 269.475 kb on - strand, within CCNA_00256 at 269.475 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.499 kb on + strand, within CCNA_00256 at 269.507 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.527 kb on - strand, within CCNA_00256 at 269.537 kb on - strand, within CCNA_00256 at 269.573 kb on + strand, within CCNA_00256 at 269.594 kb on + strand, within CCNA_00256 at 269.600 kb on - strand, within CCNA_00256 at 269.602 kb on + strand, within CCNA_00256 at 269.608 kb on + strand, within CCNA_00256 at 269.642 kb on - strand, within CCNA_00256 at 269.666 kb on + strand, within CCNA_00256 at 269.671 kb on + strand, within CCNA_00256 at 269.671 kb on + strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.701 kb on - strand, within CCNA_00256 at 269.717 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.733 kb on - strand at 269.733 kb on - strand, within CCNA_00256 at 269.733 kb on - strand, within CCNA_00256 at 269.773 kb on - strand, within CCNA_00256 at 269.773 kb on - strand, within CCNA_00256 at 269.801 kb on - strand, within CCNA_00256 at 269.819 kb on + strand, within CCNA_00256 at 269.824 kb on + strand, within CCNA_00256 at 269.827 kb on - strand, within CCNA_00256 at 269.864 kb on - strand, within CCNA_00256 at 269.872 kb on - strand, within CCNA_00256 at 269.873 kb on - strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.878 kb on + strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.925 kb on - strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.979 kb on + strand at 269.979 kb on + strand at 269.987 kb on + strand at 269.987 kb on + strand at 269.992 kb on + strand at 269.992 kb on + strand at 269.995 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.010 kb on + strand at 270.064 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.141 kb on + strand at 270.248 kb on - strand at 270.328 kb on - strand, within CCNA_00257 at 270.374 kb on - strand, within CCNA_00257 at 270.411 kb on - strand, within CCNA_00257 at 270.694 kb on - strand, within CCNA_00257 at 270.702 kb on - strand at 270.702 kb on - strand, within CCNA_00257 at 270.818 kb on - strand at 270.872 kb on - strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.911 kb on - strand, within CCNA_00257 at 270.940 kb on - strand, within CCNA_00257 at 270.955 kb on - strand, within CCNA_00257 at 270.996 kb on - strand, within CCNA_00257 at 271.123 kb on - strand, within CCNA_00257 at 271.168 kb on - strand, within CCNA_00257 at 271.248 kb on - strand, within CCNA_00257 at 271.322 kb on - strand, within CCNA_00257 at 271.355 kb on - strand at 271.410 kb on - strand, within CCNA_00257 at 271.415 kb on - strand, within CCNA_00257 at 271.458 kb on - strand at 271.602 kb on + strand at 271.612 kb on + strand at 271.761 kb on + strand at 271.766 kb on + strand at 271.766 kb on + strand at 271.769 kb on - strand at 271.769 kb on - strand at 271.776 kb on + strand at 271.776 kb on + strand at 271.809 kb on + strand at 271.817 kb on - strand at 271.834 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.847 kb on - strand at 271.847 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.862 kb on - strand at 271.869 kb on - strand at 271.870 kb on + strand at 271.870 kb on + strand at 271.877 kb on - strand at 271.877 kb on - strand at 271.878 kb on - strand at 271.910 kb on - strand at 271.918 kb on + strand at 271.918 kb on - strand at 271.926 kb on - strand at 271.927 kb on - strand at 271.970 kb on - strand at 271.978 kb on - strand at 271.978 kb on - strand at 272.004 kb on - strand, within CCNA_00259 at 272.009 kb on - strand, within CCNA_00259 at 272.044 kb on + strand, within CCNA_00259 at 272.044 kb on + strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.057 kb on - strand, within CCNA_00259 at 272.057 kb on - strand at 272.098 kb on - strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.129 kb on + strand, within CCNA_00259 at 272.135 kb on + strand, within CCNA_00259 at 272.137 kb on - strand, within CCNA_00259 at 272.183 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.207 kb on + strand, within CCNA_00259 at 272.215 kb on - strand, within CCNA_00259 at 272.255 kb on + strand, within CCNA_00259 at 272.255 kb on + strand, within CCNA_00259 at 272.268 kb on - strand, within CCNA_00259 at 272.272 kb on - strand, within CCNA_00259 at 272.296 kb on - strand, within CCNA_00259 at 272.300 kb on - strand, within CCNA_00259 at 272.357 kb on + strand, within CCNA_00259 at 272.409 kb on + strand, within CCNA_00259 at 272.448 kb on - strand, within CCNA_00259 at 272.459 kb on - strand, within CCNA_00259 at 272.492 kb on + strand, within CCNA_00259 at 272.492 kb on + strand, within CCNA_00259 at 272.511 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand at 272.534 kb on + strand, within CCNA_00259 at 272.534 kb on + strand, within CCNA_00259 at 272.535 kb on + strand, within CCNA_00259 at 272.538 kb on - strand, within CCNA_00259 at 272.542 kb on - strand, within CCNA_00259 at 272.542 kb on - strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Doxycycline hyclate 0.0001 mg/ml remove 269,211 - +0.1 269,212 - +0.5 269,287 + +0.3 269,287 + +1.6 269,312 + CCNA_00256 0.12 -1.9 269,320 - CCNA_00256 0.13 -0.4 269,356 + CCNA_00256 0.18 +0.0 269,388 - CCNA_00256 0.21 +1.2 269,429 + CCNA_00256 0.26 -1.2 269,433 - CCNA_00256 0.27 -1.2 269,437 - CCNA_00256 0.27 +0.5 269,437 - CCNA_00256 0.27 +2.0 269,446 + CCNA_00256 0.28 +0.6 269,454 - CCNA_00256 0.29 +0.6 269,467 + CCNA_00256 0.31 +0.7 269,467 + CCNA_00256 0.31 -0.4 269,475 - CCNA_00256 0.32 +1.5 269,475 - CCNA_00256 0.32 +0.7 269,490 - CCNA_00256 0.33 -0.4 269,490 - CCNA_00256 0.33 +0.4 269,499 + CCNA_00256 0.34 +1.6 269,507 - CCNA_00256 0.35 +1.4 269,512 - CCNA_00256 0.36 +0.7 269,512 - CCNA_00256 0.36 +1.5 269,512 - CCNA_00256 0.36 +1.1 269,527 - CCNA_00256 0.38 +1.6 269,537 - CCNA_00256 0.39 +0.9 269,573 + CCNA_00256 0.43 -0.7 269,594 + CCNA_00256 0.46 -1.0 269,600 - CCNA_00256 0.46 -0.2 269,602 + CCNA_00256 0.46 -0.7 269,608 + CCNA_00256 0.47 -0.4 269,642 - CCNA_00256 0.51 +2.2 269,666 + CCNA_00256 0.54 +0.5 269,671 + CCNA_00256 0.55 +0.5 269,671 + CCNA_00256 0.55 -2.0 269,679 - CCNA_00256 0.56 +3.2 269,679 - CCNA_00256 0.56 +1.1 269,679 - CCNA_00256 0.56 +0.6 269,679 - CCNA_00256 0.56 +0.6 269,701 - CCNA_00256 0.58 +1.5 269,717 + CCNA_00256 0.60 -1.4 269,725 + CCNA_00256 0.61 +0.3 269,725 + CCNA_00256 0.61 +0.6 269,725 + CCNA_00256 0.61 -0.2 269,725 + CCNA_00256 0.61 -1.2 269,733 - +0.6 269,733 - CCNA_00256 0.62 +0.4 269,733 - CCNA_00256 0.62 +1.5 269,773 - CCNA_00256 0.67 +1.2 269,773 - CCNA_00256 0.67 +1.3 269,801 - CCNA_00256 0.70 +0.5 269,819 + CCNA_00256 0.72 -2.8 269,824 + CCNA_00256 0.73 +0.3 269,827 - CCNA_00256 0.73 +1.0 269,864 - CCNA_00256 0.77 +1.1 269,872 - CCNA_00256 0.78 +2.1 269,873 - CCNA_00256 0.78 -0.6 269,876 + CCNA_00256 0.79 -2.5 269,876 + CCNA_00256 0.79 -0.6 269,876 + CCNA_00256 0.79 +0.6 269,878 + CCNA_00256 0.79 -0.9 269,884 - CCNA_00256 0.80 +0.7 269,884 - CCNA_00256 0.80 +0.3 269,884 - CCNA_00256 0.80 +1.2 269,925 - CCNA_00256 0.84 +0.7 269,969 + CCNA_00256 0.90 -2.0 269,969 + CCNA_00256 0.90 -1.5 269,969 + CCNA_00256 0.90 -0.4 269,969 + CCNA_00256 0.90 +1.4 269,969 + CCNA_00256 0.90 -0.0 269,969 + CCNA_00256 0.90 +1.0 269,977 - -0.9 269,977 - +0.7 269,977 - -0.2 269,977 - +0.9 269,977 - +0.6 269,977 - -0.9 269,977 - +1.9 269,979 + +0.6 269,979 + +1.1 269,987 + +0.3 269,987 + -0.2 269,992 + +0.3 269,992 + +0.0 269,995 - -1.3 270,000 - +0.9 270,000 - +0.1 270,000 - -0.1 270,000 - -0.7 270,000 - +0.4 270,000 - +1.0 270,000 - +1.4 270,010 + -0.2 270,064 + +0.3 270,084 + +0.9 270,084 + +0.8 270,084 + -0.1 270,084 + -1.6 270,084 + +0.4 270,084 + -1.3 270,092 - +0.3 270,092 - -0.9 270,092 - +1.3 270,092 - -0.1 270,092 - -1.2 270,092 - -1.0 270,092 - -0.2 270,092 - +0.8 270,092 - -0.6 270,092 - -0.4 270,092 - -2.5 270,092 - +0.0 270,141 + -0.4 270,248 - +2.1 270,328 - CCNA_00257 0.11 +4.7 270,374 - CCNA_00257 0.14 +1.8 270,411 - CCNA_00257 0.17 +1.7 270,694 - CCNA_00257 0.37 +2.9 270,702 - +2.9 270,702 - CCNA_00257 0.38 +0.6 270,818 - +3.7 270,872 - CCNA_00257 0.50 +2.4 270,896 - CCNA_00257 0.52 +3.4 270,896 - CCNA_00257 0.52 +2.0 270,911 - CCNA_00257 0.53 +2.8 270,940 - CCNA_00257 0.55 +1.9 270,955 - CCNA_00257 0.56 +3.2 270,996 - CCNA_00257 0.59 +2.6 271,123 - CCNA_00257 0.68 +1.8 271,168 - CCNA_00257 0.71 +2.4 271,248 - CCNA_00257 0.77 +2.2 271,322 - CCNA_00257 0.82 +3.6 271,355 - +2.6 271,410 - CCNA_00257 0.89 +0.3 271,415 - CCNA_00257 0.89 +4.5 271,458 - +2.0 271,602 + +1.2 271,612 + -0.3 271,761 + -0.1 271,766 + +0.3 271,766 + -0.9 271,769 - +0.1 271,769 - -1.4 271,776 + -0.5 271,776 + -2.6 271,809 + -0.8 271,817 - +1.0 271,834 + -0.3 271,839 + -1.6 271,839 + +0.1 271,839 + -0.7 271,844 + -0.4 271,844 + +1.6 271,844 + +0.0 271,844 + -0.7 271,844 + -0.5 271,844 + -1.1 271,844 + +0.7 271,844 + -0.4 271,844 + -0.4 271,847 - -0.2 271,847 - +0.7 271,852 - +0.3 271,852 - +0.5 271,852 - +0.3 271,852 - -1.0 271,852 - -0.6 271,852 - -1.9 271,852 - -1.9 271,852 - +0.4 271,852 - -0.5 271,852 - -0.5 271,852 - -0.5 271,852 - +0.0 271,852 - +0.2 271,852 - -0.7 271,862 - -0.4 271,869 - -0.2 271,870 + +0.0 271,870 + +1.6 271,877 - -1.3 271,877 - +0.7 271,878 - -0.6 271,910 - +0.5 271,918 + -1.2 271,918 - +1.7 271,926 - +0.5 271,927 - +0.9 271,970 - -0.6 271,978 - -1.6 271,978 - -1.9 272,004 - CCNA_00259 0.13 -1.3 272,009 - CCNA_00259 0.14 -0.2 272,044 + CCNA_00259 0.19 -1.7 272,044 + CCNA_00259 0.19 +1.6 272,049 + CCNA_00259 0.20 -1.6 272,057 - CCNA_00259 0.21 -1.2 272,057 - +0.6 272,098 - CCNA_00259 0.26 -1.3 272,110 + CCNA_00259 0.28 +0.0 272,110 + CCNA_00259 0.28 +0.1 272,117 + CCNA_00259 0.29 +0.6 272,117 + CCNA_00259 0.29 +1.6 272,117 + CCNA_00259 0.29 -0.7 272,125 - CCNA_00259 0.30 +0.4 272,125 - CCNA_00259 0.30 +0.5 272,125 - CCNA_00259 0.30 -1.1 272,125 - CCNA_00259 0.30 -1.9 272,129 + CCNA_00259 0.31 -0.7 272,135 + CCNA_00259 0.32 -1.9 272,137 - CCNA_00259 0.32 -1.6 272,183 + CCNA_00259 0.38 +0.0 272,188 + CCNA_00259 0.39 -0.2 272,188 + CCNA_00259 0.39 -0.7 272,188 + CCNA_00259 0.39 -0.4 272,191 - CCNA_00259 0.40 -0.3 272,191 - CCNA_00259 0.40 -0.8 272,191 - CCNA_00259 0.40 +0.7 272,207 + CCNA_00259 0.42 +0.0 272,215 - CCNA_00259 0.43 -2.2 272,255 + CCNA_00259 0.49 +1.0 272,255 + CCNA_00259 0.49 +2.2 272,268 - CCNA_00259 0.50 +0.6 272,272 - CCNA_00259 0.51 -0.3 272,296 - CCNA_00259 0.54 -0.7 272,300 - CCNA_00259 0.55 -2.5 272,357 + CCNA_00259 0.63 -2.1 272,409 + CCNA_00259 0.70 -2.1 272,448 - CCNA_00259 0.76 +0.0 272,459 - CCNA_00259 0.77 +0.6 272,492 + CCNA_00259 0.82 -0.2 272,492 + CCNA_00259 0.82 -1.3 272,511 + CCNA_00259 0.85 +1.3 272,522 + CCNA_00259 0.86 -1.1 272,522 + CCNA_00259 0.86 -0.1 272,530 - CCNA_00259 0.87 +1.6 272,530 - CCNA_00259 0.87 -1.4 272,530 - CCNA_00259 0.87 -0.2 272,530 - +0.0 272,534 + CCNA_00259 0.88 -1.6 272,534 + CCNA_00259 0.88 -1.1 272,535 + CCNA_00259 0.88 -1.0 272,538 - CCNA_00259 0.88 -1.4 272,542 - CCNA_00259 0.89 -0.4 272,542 - CCNA_00259 0.89 -0.5 272,543 + CCNA_00259 0.89 -1.7 272,543 + CCNA_00259 0.89 +0.0 272,543 + CCNA_00259 0.89 +1.2 272,554 - -0.4
Or see this region's nucleotide sequence