Experiment: PYE with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00011 and CCNA_00012 are separated by 86 nucleotides CCNA_00012 and CCNA_00013 are separated by 20 nucleotides
CCNA_00011: CCNA_00011 - chaperone protein DnaJ, at 10,223 to 11,380
_00011
CCNA_00012: CCNA_00012 - DNA mismatch repair protein MutS, at 11,467 to 14,178
_00012
CCNA_00013: CCNA_00013 - protein-PII uridylyltransferase, at 14,199 to 17,021
_00013
Position (kb) 11
12
13
14
15 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 10.759 kb on - strand, within CCNA_00011 at 10.963 kb on + strand, within CCNA_00011 at 11.012 kb on + strand, within CCNA_00011 at 11.057 kb on + strand, within CCNA_00011 at 11.096 kb on + strand, within CCNA_00011 at 11.155 kb on + strand, within CCNA_00011 at 11.184 kb on - strand, within CCNA_00011 at 11.190 kb on - strand, within CCNA_00011 at 11.247 kb on + strand, within CCNA_00011 at 11.273 kb on + strand at 11.317 kb on + strand at 11.317 kb on - strand at 11.325 kb on - strand at 11.344 kb on + strand at 11.361 kb on - strand at 11.366 kb on + strand at 11.372 kb on - strand at 11.382 kb on - strand at 11.383 kb on - strand at 11.446 kb on + strand at 11.497 kb on - strand at 11.646 kb on - strand at 11.716 kb on + strand at 11.751 kb on + strand, within CCNA_00012 at 11.775 kb on + strand, within CCNA_00012 at 11.807 kb on + strand, within CCNA_00012 at 11.807 kb on + strand, within CCNA_00012 at 11.813 kb on + strand, within CCNA_00012 at 11.815 kb on - strand, within CCNA_00012 at 11.934 kb on - strand, within CCNA_00012 at 11.955 kb on + strand, within CCNA_00012 at 11.967 kb on + strand, within CCNA_00012 at 11.976 kb on - strand, within CCNA_00012 at 11.991 kb on + strand, within CCNA_00012 at 11.999 kb on - strand, within CCNA_00012 at 12.002 kb on + strand, within CCNA_00012 at 12.024 kb on - strand, within CCNA_00012 at 12.027 kb on + strand, within CCNA_00012 at 12.029 kb on + strand, within CCNA_00012 at 12.035 kb on - strand, within CCNA_00012 at 12.081 kb on - strand, within CCNA_00012 at 12.082 kb on + strand, within CCNA_00012 at 12.082 kb on + strand, within CCNA_00012 at 12.098 kb on + strand, within CCNA_00012 at 12.098 kb on + strand, within CCNA_00012 at 12.098 kb on + strand, within CCNA_00012 at 12.108 kb on + strand, within CCNA_00012 at 12.111 kb on - strand, within CCNA_00012 at 12.116 kb on - strand, within CCNA_00012 at 12.116 kb on - strand, within CCNA_00012 at 12.116 kb on - strand, within CCNA_00012 at 12.116 kb on - strand, within CCNA_00012 at 12.121 kb on + strand, within CCNA_00012 at 12.123 kb on - strand, within CCNA_00012 at 12.126 kb on + strand, within CCNA_00012 at 12.134 kb on - strand, within CCNA_00012 at 12.150 kb on - strand, within CCNA_00012 at 12.162 kb on + strand, within CCNA_00012 at 12.170 kb on - strand, within CCNA_00012 at 12.170 kb on - strand, within CCNA_00012 at 12.172 kb on + strand, within CCNA_00012 at 12.172 kb on + strand, within CCNA_00012 at 12.178 kb on + strand, within CCNA_00012 at 12.205 kb on - strand, within CCNA_00012 at 12.221 kb on + strand, within CCNA_00012 at 12.316 kb on - strand, within CCNA_00012 at 12.349 kb on - strand, within CCNA_00012 at 12.387 kb on - strand, within CCNA_00012 at 12.459 kb on + strand, within CCNA_00012 at 12.478 kb on + strand, within CCNA_00012 at 12.496 kb on + strand, within CCNA_00012 at 12.546 kb on + strand, within CCNA_00012 at 12.546 kb on + strand, within CCNA_00012 at 12.552 kb on - strand, within CCNA_00012 at 12.562 kb on - strand, within CCNA_00012 at 12.594 kb on + strand, within CCNA_00012 at 12.612 kb on - strand, within CCNA_00012 at 12.621 kb on - strand, within CCNA_00012 at 12.635 kb on + strand, within CCNA_00012 at 12.643 kb on - strand, within CCNA_00012 at 12.647 kb on + strand, within CCNA_00012 at 12.702 kb on + strand, within CCNA_00012 at 12.707 kb on + strand, within CCNA_00012 at 12.719 kb on + strand, within CCNA_00012 at 12.719 kb on + strand, within CCNA_00012 at 12.719 kb on + strand, within CCNA_00012 at 12.734 kb on + strand, within CCNA_00012 at 12.734 kb on + strand, within CCNA_00012 at 12.737 kb on - strand, within CCNA_00012 at 12.737 kb on - strand, within CCNA_00012 at 12.737 kb on - strand, within CCNA_00012 at 12.742 kb on - strand, within CCNA_00012 at 12.744 kb on + strand, within CCNA_00012 at 12.744 kb on + strand, within CCNA_00012 at 12.744 kb on + strand, within CCNA_00012 at 12.752 kb on - strand, within CCNA_00012 at 12.752 kb on - strand, within CCNA_00012 at 12.752 kb on - strand, within CCNA_00012 at 12.752 kb on - strand, within CCNA_00012 at 12.773 kb on + strand, within CCNA_00012 at 12.781 kb on - strand, within CCNA_00012 at 12.791 kb on + strand, within CCNA_00012 at 12.855 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.861 kb on + strand, within CCNA_00012 at 12.869 kb on - strand, within CCNA_00012 at 12.869 kb on - strand, within CCNA_00012 at 12.869 kb on - strand, within CCNA_00012 at 12.892 kb on - strand, within CCNA_00012 at 12.951 kb on + strand, within CCNA_00012 at 12.959 kb on - strand, within CCNA_00012 at 13.004 kb on - strand, within CCNA_00012 at 13.031 kb on + strand, within CCNA_00012 at 13.041 kb on + strand, within CCNA_00012 at 13.043 kb on + strand, within CCNA_00012 at 13.051 kb on - strand, within CCNA_00012 at 13.076 kb on + strand, within CCNA_00012 at 13.133 kb on + strand, within CCNA_00012 at 13.133 kb on + strand, within CCNA_00012 at 13.133 kb on + strand, within CCNA_00012 at 13.141 kb on - strand, within CCNA_00012 at 13.168 kb on - strand, within CCNA_00012 at 13.168 kb on - strand, within CCNA_00012 at 13.170 kb on + strand, within CCNA_00012 at 13.178 kb on - strand, within CCNA_00012 at 13.210 kb on + strand, within CCNA_00012 at 13.210 kb on + strand, within CCNA_00012 at 13.218 kb on - strand, within CCNA_00012 at 13.218 kb on - strand, within CCNA_00012 at 13.232 kb on + strand, within CCNA_00012 at 13.232 kb on + strand, within CCNA_00012 at 13.270 kb on + strand, within CCNA_00012 at 13.282 kb on + strand, within CCNA_00012 at 13.283 kb on - strand, within CCNA_00012 at 13.299 kb on - strand, within CCNA_00012 at 13.313 kb on - strand, within CCNA_00012 at 13.359 kb on + strand, within CCNA_00012 at 13.359 kb on + strand, within CCNA_00012 at 13.359 kb on + strand, within CCNA_00012 at 13.359 kb on + strand, within CCNA_00012 at 13.367 kb on - strand, within CCNA_00012 at 13.381 kb on + strand, within CCNA_00012 at 13.429 kb on + strand, within CCNA_00012 at 13.434 kb on + strand, within CCNA_00012 at 13.437 kb on - strand, within CCNA_00012 at 13.551 kb on - strand, within CCNA_00012 at 13.574 kb on + strand, within CCNA_00012 at 13.594 kb on + strand, within CCNA_00012 at 13.611 kb on + strand, within CCNA_00012 at 13.622 kb on + strand, within CCNA_00012 at 13.632 kb on + strand, within CCNA_00012 at 13.660 kb on - strand, within CCNA_00012 at 13.687 kb on + strand, within CCNA_00012 at 13.702 kb on - strand, within CCNA_00012 at 13.716 kb on + strand, within CCNA_00012 at 13.719 kb on - strand, within CCNA_00012 at 13.719 kb on - strand, within CCNA_00012 at 13.724 kb on - strand, within CCNA_00012 at 13.724 kb on - strand, within CCNA_00012 at 13.724 kb on - strand, within CCNA_00012 at 13.783 kb on - strand, within CCNA_00012 at 13.783 kb on - strand, within CCNA_00012 at 13.789 kb on + strand, within CCNA_00012 at 13.790 kb on + strand, within CCNA_00012 at 13.801 kb on + strand, within CCNA_00012 at 13.809 kb on - strand, within CCNA_00012 at 13.877 kb on + strand, within CCNA_00012 at 13.904 kb on + strand, within CCNA_00012 at 13.904 kb on + strand, within CCNA_00012 at 13.948 kb on - strand at 13.955 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.971 kb on - strand at 14.041 kb on - strand at 14.045 kb on - strand at 14.059 kb on - strand at 14.061 kb on + strand at 14.075 kb on - strand at 14.117 kb on + strand at 14.117 kb on + strand at 14.117 kb on + strand at 14.117 kb on + strand at 14.125 kb on - strand at 14.125 kb on - strand at 14.154 kb on + strand at 14.154 kb on + strand at 14.162 kb on - strand at 14.162 kb on - strand at 14.162 kb on - strand at 14.166 kb on + strand at 14.166 kb on + strand at 14.166 kb on + strand at 14.166 kb on + strand at 14.166 kb on + strand at 14.166 kb on + strand at 14.174 kb on - strand at 14.174 kb on - strand at 14.181 kb on - strand at 14.181 kb on - strand at 14.185 kb on + strand at 14.211 kb on - strand at 14.242 kb on - strand at 14.253 kb on - strand at 14.261 kb on - strand at 14.261 kb on - strand at 14.264 kb on + strand at 14.397 kb on + strand at 14.422 kb on - strand at 14.456 kb on - strand at 14.507 kb on + strand, within CCNA_00013 at 14.545 kb on + strand, within CCNA_00013 at 14.545 kb on + strand, within CCNA_00013 at 14.547 kb on - strand, within CCNA_00013 at 14.587 kb on - strand, within CCNA_00013 at 14.771 kb on + strand, within CCNA_00013 at 14.850 kb on - strand, within CCNA_00013 at 14.899 kb on + strand, within CCNA_00013 at 14.906 kb on + strand, within CCNA_00013 at 14.984 kb on + strand, within CCNA_00013 at 15.033 kb on + strand, within CCNA_00013 at 15.083 kb on - strand, within CCNA_00013 at 15.108 kb on + strand, within CCNA_00013 at 15.110 kb on - strand, within CCNA_00013 at 15.116 kb on - strand, within CCNA_00013 at 15.123 kb on - strand, within CCNA_00013
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Doxycycline hyclate 0.0001 mg/ml remove 10,759 - CCNA_00011 0.46 +4.7 10,963 + CCNA_00011 0.64 +6.5 11,012 + CCNA_00011 0.68 +5.7 11,057 + CCNA_00011 0.72 +6.6 11,096 + CCNA_00011 0.75 +6.3 11,155 + CCNA_00011 0.80 +6.7 11,184 - CCNA_00011 0.83 +4.5 11,190 - CCNA_00011 0.84 +6.7 11,247 + CCNA_00011 0.88 +6.4 11,273 + +6.9 11,317 + +3.6 11,317 - +3.7 11,325 - +4.6 11,344 + +4.2 11,361 - +1.8 11,366 + +3.6 11,372 - +1.2 11,382 - -0.4 11,383 - -0.4 11,446 + +2.4 11,497 - +1.2 11,646 - -0.6 11,716 + +0.9 11,751 + CCNA_00012 0.10 +0.8 11,775 + CCNA_00012 0.11 +1.7 11,807 + CCNA_00012 0.13 +1.6 11,807 + CCNA_00012 0.13 +1.5 11,813 + CCNA_00012 0.13 +2.0 11,815 - CCNA_00012 0.13 -0.7 11,934 - CCNA_00012 0.17 +0.2 11,955 + CCNA_00012 0.18 +0.7 11,967 + CCNA_00012 0.18 +0.5 11,976 - CCNA_00012 0.19 +0.3 11,991 + CCNA_00012 0.19 +0.9 11,999 - CCNA_00012 0.20 +0.1 12,002 + CCNA_00012 0.20 +1.4 12,024 - CCNA_00012 0.21 +1.6 12,027 + CCNA_00012 0.21 +1.1 12,029 + CCNA_00012 0.21 +0.4 12,035 - CCNA_00012 0.21 +2.8 12,081 - CCNA_00012 0.23 +0.9 12,082 + CCNA_00012 0.23 -0.5 12,082 + CCNA_00012 0.23 +1.8 12,098 + CCNA_00012 0.23 -0.3 12,098 + CCNA_00012 0.23 +0.1 12,098 + CCNA_00012 0.23 +0.9 12,108 + CCNA_00012 0.24 +1.9 12,111 - CCNA_00012 0.24 -0.3 12,116 - CCNA_00012 0.24 -0.9 12,116 - CCNA_00012 0.24 +0.6 12,116 - CCNA_00012 0.24 +1.6 12,116 - CCNA_00012 0.24 -0.2 12,121 + CCNA_00012 0.24 +0.3 12,123 - CCNA_00012 0.24 +2.3 12,126 + CCNA_00012 0.24 -0.0 12,134 - CCNA_00012 0.25 +0.0 12,150 - CCNA_00012 0.25 +1.6 12,162 + CCNA_00012 0.26 +1.4 12,170 - CCNA_00012 0.26 +0.4 12,170 - CCNA_00012 0.26 +0.6 12,172 + CCNA_00012 0.26 +1.7 12,172 + CCNA_00012 0.26 +1.1 12,178 + CCNA_00012 0.26 -1.7 12,205 - CCNA_00012 0.27 -2.3 12,221 + CCNA_00012 0.28 +0.4 12,316 - CCNA_00012 0.31 +0.3 12,349 - CCNA_00012 0.33 -0.2 12,387 - CCNA_00012 0.34 +0.6 12,459 + CCNA_00012 0.37 +0.7 12,478 + CCNA_00012 0.37 +1.1 12,496 + CCNA_00012 0.38 +0.5 12,546 + CCNA_00012 0.40 +0.6 12,546 + CCNA_00012 0.40 +0.7 12,552 - CCNA_00012 0.40 -2.3 12,562 - CCNA_00012 0.40 -0.1 12,594 + CCNA_00012 0.42 +1.6 12,612 - CCNA_00012 0.42 +1.3 12,621 - CCNA_00012 0.43 -0.7 12,635 + CCNA_00012 0.43 -0.5 12,643 - CCNA_00012 0.43 +3.2 12,647 + CCNA_00012 0.44 +1.3 12,702 + CCNA_00012 0.46 -0.7 12,707 + CCNA_00012 0.46 +0.1 12,719 + CCNA_00012 0.46 +1.5 12,719 + CCNA_00012 0.46 +1.5 12,719 + CCNA_00012 0.46 +0.7 12,734 + CCNA_00012 0.47 +1.6 12,734 + CCNA_00012 0.47 +1.5 12,737 - CCNA_00012 0.47 +0.5 12,737 - CCNA_00012 0.47 +0.6 12,737 - CCNA_00012 0.47 -0.8 12,742 - CCNA_00012 0.47 -0.4 12,744 + CCNA_00012 0.47 -0.1 12,744 + CCNA_00012 0.47 +0.3 12,744 + CCNA_00012 0.47 +0.1 12,752 - CCNA_00012 0.47 -2.4 12,752 - CCNA_00012 0.47 +0.5 12,752 - CCNA_00012 0.47 +1.4 12,752 - CCNA_00012 0.47 -0.5 12,773 + CCNA_00012 0.48 +1.1 12,781 - CCNA_00012 0.48 +0.8 12,791 + CCNA_00012 0.49 +0.3 12,855 + CCNA_00012 0.51 +1.2 12,861 + CCNA_00012 0.51 +1.3 12,861 + CCNA_00012 0.51 -0.4 12,861 + CCNA_00012 0.51 -0.1 12,861 + CCNA_00012 0.51 +0.5 12,861 + CCNA_00012 0.51 +1.9 12,861 + CCNA_00012 0.51 +2.6 12,869 - CCNA_00012 0.52 +1.6 12,869 - CCNA_00012 0.52 +1.8 12,869 - CCNA_00012 0.52 -1.6 12,892 - CCNA_00012 0.53 -0.2 12,951 + CCNA_00012 0.55 +0.1 12,959 - CCNA_00012 0.55 +1.6 13,004 - CCNA_00012 0.57 -0.7 13,031 + CCNA_00012 0.58 +0.9 13,041 + CCNA_00012 0.58 +2.7 13,043 + CCNA_00012 0.58 -0.5 13,051 - CCNA_00012 0.58 -1.0 13,076 + CCNA_00012 0.59 +0.9 13,133 + CCNA_00012 0.61 +0.0 13,133 + CCNA_00012 0.61 +1.3 13,133 + CCNA_00012 0.61 +1.6 13,141 - CCNA_00012 0.62 +1.1 13,168 - CCNA_00012 0.63 -0.3 13,168 - CCNA_00012 0.63 -0.1 13,170 + CCNA_00012 0.63 +0.5 13,178 - CCNA_00012 0.63 -2.2 13,210 + CCNA_00012 0.64 +0.8 13,210 + CCNA_00012 0.64 -0.7 13,218 - CCNA_00012 0.65 -0.2 13,218 - CCNA_00012 0.65 -1.9 13,232 + CCNA_00012 0.65 +1.0 13,232 + CCNA_00012 0.65 +0.8 13,270 + CCNA_00012 0.66 +1.0 13,282 + CCNA_00012 0.67 +0.9 13,283 - CCNA_00012 0.67 +1.3 13,299 - CCNA_00012 0.68 -0.8 13,313 - CCNA_00012 0.68 +1.1 13,359 + CCNA_00012 0.70 +0.8 13,359 + CCNA_00012 0.70 +0.3 13,359 + CCNA_00012 0.70 +1.7 13,359 + CCNA_00012 0.70 +0.5 13,367 - CCNA_00012 0.70 +1.0 13,381 + CCNA_00012 0.71 +1.1 13,429 + CCNA_00012 0.72 +1.4 13,434 + CCNA_00012 0.73 +1.3 13,437 - CCNA_00012 0.73 +0.1 13,551 - CCNA_00012 0.77 +1.0 13,574 + CCNA_00012 0.78 -0.2 13,594 + CCNA_00012 0.78 -0.0 13,611 + CCNA_00012 0.79 +0.4 13,622 + CCNA_00012 0.79 +1.3 13,632 + CCNA_00012 0.80 +0.9 13,660 - CCNA_00012 0.81 -0.9 13,687 + CCNA_00012 0.82 +1.3 13,702 - CCNA_00012 0.82 -0.1 13,716 + CCNA_00012 0.83 -0.0 13,719 - CCNA_00012 0.83 -0.1 13,719 - CCNA_00012 0.83 -0.8 13,724 - CCNA_00012 0.83 -3.8 13,724 - CCNA_00012 0.83 +1.3 13,724 - CCNA_00012 0.83 -0.9 13,783 - CCNA_00012 0.85 +0.8 13,783 - CCNA_00012 0.85 -0.4 13,789 + CCNA_00012 0.86 +1.2 13,790 + CCNA_00012 0.86 +0.2 13,801 + CCNA_00012 0.86 +0.7 13,809 - CCNA_00012 0.86 +0.0 13,877 + CCNA_00012 0.89 +0.8 13,904 + CCNA_00012 0.90 +1.1 13,904 + CCNA_00012 0.90 +0.4 13,948 - +3.0 13,955 + +0.5 13,963 + +1.9 13,963 + +0.5 13,963 + +0.6 13,963 + +0.0 13,963 + +1.0 13,971 - +0.1 14,041 - +0.1 14,045 - +2.9 14,059 - +3.2 14,061 + +0.5 14,075 - -1.4 14,117 + +0.7 14,117 + +2.2 14,117 + +0.9 14,117 + +1.0 14,125 - +0.8 14,125 - -1.2 14,154 + +0.5 14,154 + -0.4 14,162 - +1.7 14,162 - +0.2 14,162 - +0.5 14,166 + +0.2 14,166 + +0.2 14,166 + +0.2 14,166 + +0.8 14,166 + -0.4 14,166 + +3.7 14,174 - +0.3 14,174 - +1.0 14,181 - -0.6 14,181 - +1.3 14,185 + -0.0 14,211 - +1.6 14,242 - -2.4 14,253 - +1.7 14,261 - -1.7 14,261 - -0.4 14,264 + -0.9 14,397 + +2.6 14,422 - +2.1 14,456 - +1.9 14,507 + CCNA_00013 0.11 +1.5 14,545 + CCNA_00013 0.12 +2.3 14,545 + CCNA_00013 0.12 +3.0 14,547 - CCNA_00013 0.12 +2.6 14,587 - CCNA_00013 0.14 +2.0 14,771 + CCNA_00013 0.20 +2.9 14,850 - CCNA_00013 0.23 +1.1 14,899 + CCNA_00013 0.25 +2.2 14,906 + CCNA_00013 0.25 +1.0 14,984 + CCNA_00013 0.28 +2.4 15,033 + CCNA_00013 0.30 +2.0 15,083 - CCNA_00013 0.31 +0.3 15,108 + CCNA_00013 0.32 +1.6 15,110 - CCNA_00013 0.32 +0.6 15,116 - CCNA_00013 0.32 +1.9 15,123 - CCNA_00013 0.33 +1.5
Or see this region's nucleotide sequence