Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and Shew_2336 are separated by 39 nucleotides Shew_2336 and tgt are separated by 84 nucleotides tgt and Shew_2338 are separated by 168 nucleotides
Shew_2335: secD - preprotein translocase subunit SecD (RefSeq), at 2,728,326 to 2,730,176
secD
Shew_2336: Shew_2336 - preprotein translocase, YajC subunit (RefSeq), at 2,730,216 to 2,730,491
_2336
Shew_2337: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 2,730,576 to 2,731,700
tgt
Shew_2338: Shew_2338 - S-adenosylmethionine--tRNA-ribosyltransferase-isomerase (RefSeq), at 2,731,869 to 2,732,906
_2338
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-1
0
1
2 at 2730.792 kb on - strand, within tgt at 2731.167 kb on - strand, within tgt at 2731.179 kb on + strand, within tgt at 2731.192 kb on + strand, within tgt at 2731.212 kb on - strand, within tgt at 2731.297 kb on - strand, within tgt at 2731.363 kb on - strand, within tgt at 2731.497 kb on + strand, within tgt at 2731.830 kb on + strand at 2731.830 kb on + strand at 2731.838 kb on - strand at 2731.941 kb on - strand at 2732.617 kb on - strand, within Shew_2338
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,730,792 - tgt Shew_2337 0.19 +0.6 2,731,167 - tgt Shew_2337 0.53 +0.7 2,731,179 + tgt Shew_2337 0.54 +1.9 2,731,192 + tgt Shew_2337 0.55 +0.8 2,731,212 - tgt Shew_2337 0.57 +0.9 2,731,297 - tgt Shew_2337 0.64 +0.4 2,731,363 - tgt Shew_2337 0.70 +0.6 2,731,497 + tgt Shew_2337 0.82 +1.8 2,731,830 + +0.1 2,731,830 + +0.2 2,731,838 - +0.0 2,731,941 - -0.6 2,732,617 - Shew_2338 0.72 +0.9
Or see this region's nucleotide sequence