Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2084 and Shew_2085 are separated by 2 nucleotides Shew_2085 and Shew_2086 overlap by 38 nucleotides Shew_2086 and Shew_2087 overlap by 26 nucleotides
Shew_2084: Shew_2084 - gonadoliberin III-related protein (RefSeq), at 2,410,221 to 2,411,726
_2084
Shew_2085: Shew_2085 - alpha-L-glutamate ligase-like protein (RefSeq), at 2,411,729 to 2,412,685
_2085
Shew_2086: Shew_2086 - hypothetical protein (RefSeq), at 2,412,648 to 2,414,423
_2086
Shew_2087: Shew_2087 - hypothetical protein (RefSeq), at 2,414,398 to 2,415,549
_2087
Position (kb)
2412
2413
2414
2415 Strain fitness (log2 ratio)
-1
0
1 at 2412.259 kb on + strand, within Shew_2085 at 2412.420 kb on + strand, within Shew_2085 at 2414.038 kb on - strand, within Shew_2086 at 2414.162 kb on - strand, within Shew_2086 at 2414.281 kb on - strand at 2414.332 kb on - strand at 2415.041 kb on - strand, within Shew_2087 at 2415.351 kb on - strand, within Shew_2087 at 2415.385 kb on + strand, within Shew_2087
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,412,259 + Shew_2085 0.55 +0.1 2,412,420 + Shew_2085 0.72 +0.3 2,414,038 - Shew_2086 0.78 +1.4 2,414,162 - Shew_2086 0.85 +0.7 2,414,281 - +0.5 2,414,332 - +0.9 2,415,041 - Shew_2087 0.56 +0.8 2,415,351 - Shew_2087 0.83 +1.2 2,415,385 + Shew_2087 0.86 +0.8
Or see this region's nucleotide sequence