Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1818 and Shew_1819 are separated by 397 nucleotides Shew_1819 and prpB are separated by 12 nucleotides prpB and Shew_1821 are separated by 101 nucleotides
Shew_1818: Shew_1818 - ATP-dependent protease (RefSeq), at 2,090,202 to 2,091,911
_1818
Shew_1819: Shew_1819 - GntR family transcriptional regulator (RefSeq), at 2,092,309 to 2,092,977
_1819
Shew_1820: prpB - 2-methylisocitrate lyase (RefSeq), at 2,092,990 to 2,093,868
prpB
Shew_1821: Shew_1821 - 2-methylcitrate synthase/citrate synthase II (RefSeq), at 2,093,970 to 2,095,094
_1821
Position (kb)
2092
2093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2091.591 kb on + strand, within Shew_1818 at 2092.365 kb on - strand at 2092.365 kb on - strand at 2092.732 kb on + strand, within Shew_1819 at 2093.552 kb on - strand, within prpB at 2093.582 kb on + strand, within prpB at 2093.677 kb on - strand, within prpB at 2093.681 kb on + strand, within prpB at 2093.702 kb on - strand, within prpB at 2093.933 kb on + strand at 2093.933 kb on + strand at 2093.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,091,591 + Shew_1818 0.81 -2.8 2,092,365 - +0.6 2,092,365 - +0.2 2,092,732 + Shew_1819 0.63 +2.3 2,093,552 - prpB Shew_1820 0.64 +0.4 2,093,582 + prpB Shew_1820 0.67 +2.1 2,093,677 - prpB Shew_1820 0.78 -0.3 2,093,681 + prpB Shew_1820 0.79 +2.2 2,093,702 - prpB Shew_1820 0.81 +0.7 2,093,933 + +0.8 2,093,933 + -0.2 2,093,941 - -0.1
Or see this region's nucleotide sequence