Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1081 and Shew_1082 are separated by 358 nucleotides Shew_1082 and Shew_1083 overlap by 4 nucleotides Shew_1083 and Shew_1084 are separated by 120 nucleotides
Shew_1081: Shew_1081 - threonine synthase (RefSeq), at 1,245,127 to 1,246,410
_1081
Shew_1082: Shew_1082 - hypothetical protein (RefSeq), at 1,246,769 to 1,247,065
_1082
Shew_1083: Shew_1083 - apurinic endonuclease Apn1 (RefSeq), at 1,247,062 to 1,247,913
_1083
Shew_1084: Shew_1084 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 1,248,034 to 1,250,070
_1084
Position (kb)
1247
1248 Strain fitness (log2 ratio)
-1
0
1 at 1246.332 kb on + strand at 1246.340 kb on - strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.385 kb on - strand at 1246.950 kb on - strand, within Shew_1082 at 1247.053 kb on - strand at 1247.445 kb on + strand, within Shew_1083 at 1247.717 kb on + strand, within Shew_1083 at 1247.731 kb on - strand, within Shew_1083 at 1247.831 kb on + strand at 1248.046 kb on - strand at 1248.169 kb on - strand at 1248.278 kb on + strand, within Shew_1084
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,246,332 + +0.3 1,246,340 - +0.2 1,246,377 + +0.2 1,246,377 + +0.1 1,246,377 + -0.3 1,246,385 - +0.0 1,246,950 - Shew_1082 0.61 +0.1 1,247,053 - +0.1 1,247,445 + Shew_1083 0.45 -0.4 1,247,717 + Shew_1083 0.77 -0.2 1,247,731 - Shew_1083 0.79 -0.9 1,247,831 + +0.4 1,248,046 - +0.2 1,248,169 - +0.5 1,248,278 + Shew_1084 0.12 +0.2
Or see this region's nucleotide sequence