Experiment: MoLS4 with L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF35977 and tRNA-Arg are separated by 114 nucleotides tRNA-Arg and tRNA-Arg are separated by 19 nucleotides tRNA-Arg and tRNA-Arg are separated by 32 nucleotides tRNA-Arg and DORF11999 are separated by 47 nucleotides DORF11999 and cysD are separated by 76 nucleotides cysD and DVU1567 are separated by 9 nucleotides DVU1567 and ftn are separated by 163 nucleotides ftn and porA are separated by 72 nucleotides
DORF35977: DORF35977 - no description, at 1,647,759 to 1,648,106
DORF35977
DVU_tRNA-Arg-1: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,221 to 1,648,297
tRNA-Arg
DVU_tRNA-Arg-2: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,317 to 1,648,393
tRNA-Arg
DVU_tRNA-Arg-3: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,426 to 1,648,502
tRNA-Arg
DORF11999: DORF11999 - SEED: FIG00606676: hypothetical protein, at 1,648,550 to 1,648,987
DORF11999
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
DVU1567: DVU1567 - hypothetical protein (TIGR), at 1,649,748 to 1,649,960
DVU1567
DVU1568: ftn - ferritin (TIGR), at 1,650,124 to 1,650,633
ftn
DVU1569: porA - pyruvate ferredoxin oxidoreductase, alpha subunit (TIGR), at 1,650,706 to 1,652,442
porA
Position (kb) 1649
1650 Strain fitness (log2 ratio) -2
-1
0
1 at 1648.258 kb on - strand, within tRNA-Arg at 1648.258 kb on - strand, within tRNA-Arg at 1648.758 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.855 kb on + strand, within DORF11999 at 1648.871 kb on + strand, within DORF11999 at 1648.893 kb on + strand, within DORF11999 at 1649.111 kb on - strand at 1649.150 kb on + strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.298 kb on + strand, within cysD at 1649.306 kb on - strand, within cysD at 1649.344 kb on + strand, within cysD at 1649.356 kb on - strand, within cysD at 1649.361 kb on - strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.615 kb on + strand, within cysD at 1649.632 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.640 kb on + strand, within cysD at 1649.650 kb on + strand, within cysD at 1649.745 kb on + strand at 1649.745 kb on + strand at 1649.747 kb on + strand at 1649.753 kb on - strand at 1649.769 kb on - strand at 1649.883 kb on + strand, within DVU1567 at 1649.978 kb on - strand at 1649.978 kb on - strand at 1649.994 kb on + strand at 1650.002 kb on - strand at 1650.026 kb on - strand at 1650.060 kb on + strand at 1650.060 kb on + strand at 1650.068 kb on - strand at 1650.170 kb on - strand at 1650.328 kb on + strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.383 kb on - strand, within ftn at 1650.386 kb on + strand, within ftn at 1650.408 kb on + strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.517 kb on - strand, within ftn at 1650.578 kb on - strand, within ftn at 1650.595 kb on - strand at 1650.685 kb on + strand at 1650.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan (N) remove 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 -1.1 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 +0.0 1,648,758 - DORF11999 0.47 -0.0 1,648,853 - DORF11999 0.69 -0.2 1,648,853 - DORF11999 0.69 -0.7 1,648,855 + DORF11999 0.70 -0.1 1,648,871 + DORF11999 0.73 +0.4 1,648,893 + DORF11999 0.78 -2.1 1,649,111 - +0.7 1,649,150 + cysD DVU1566 0.13 -0.2 1,649,196 - cysD DVU1566 0.20 -0.1 1,649,196 - cysD DVU1566 0.20 +0.1 1,649,298 + cysD DVU1566 0.35 +0.3 1,649,306 - cysD DVU1566 0.36 +0.1 1,649,344 + cysD DVU1566 0.41 +0.6 1,649,356 - cysD DVU1566 0.43 +0.2 1,649,361 - cysD DVU1566 0.44 +0.2 1,649,471 + cysD DVU1566 0.60 -0.2 1,649,471 + cysD DVU1566 0.60 +1.3 1,649,615 + cysD DVU1566 0.82 -0.1 1,649,632 + cysD DVU1566 0.84 -0.0 1,649,639 + cysD DVU1566 0.85 +0.5 1,649,639 + cysD DVU1566 0.85 -1.0 1,649,640 + cysD DVU1566 0.85 +0.7 1,649,650 + cysD DVU1566 0.87 +1.0 1,649,745 + -0.2 1,649,745 + -0.2 1,649,747 + +0.0 1,649,753 - -0.0 1,649,769 - -0.2 1,649,883 + DVU1567 0.63 +0.0 1,649,978 - +0.0 1,649,978 - -1.5 1,649,994 + -1.7 1,650,002 - +0.2 1,650,026 - +0.8 1,650,060 + -0.4 1,650,060 + +0.8 1,650,068 - -0.3 1,650,170 - +0.2 1,650,328 + ftn DVU1568 0.40 -0.5 1,650,338 - ftn DVU1568 0.42 -0.4 1,650,338 - ftn DVU1568 0.42 +0.0 1,650,383 - ftn DVU1568 0.51 +0.1 1,650,386 + ftn DVU1568 0.51 +0.4 1,650,408 + ftn DVU1568 0.56 -0.3 1,650,512 - ftn DVU1568 0.76 +0.5 1,650,512 - ftn DVU1568 0.76 -0.0 1,650,517 - ftn DVU1568 0.77 -0.2 1,650,578 - ftn DVU1568 0.89 -0.1 1,650,595 - +0.5 1,650,685 + -0.5 1,650,685 + -0.5
Or see this region's nucleotide sequence